Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3351 and EX28DRAFT_3352 are separated by 29 nucleotides EX28DRAFT_3352 and EX28DRAFT_3353 are separated by 10 nucleotides EX28DRAFT_3353 and EX28DRAFT_3354 are separated by 10 nucleotides EX28DRAFT_3354 and EX28DRAFT_3355 overlap by 8 nucleotides
EX28DRAFT_3351: EX28DRAFT_3351 - Transcriptional regulator, at 17,933 to 18,808
_3351
EX28DRAFT_3352: EX28DRAFT_3352 - succinate dehydrogenase subunit D (EC 1.3.5.1), at 18,838 to 19,197
_3352
EX28DRAFT_3353: EX28DRAFT_3353 - Fumarate reductase subunit C, at 19,208 to 19,603
_3353
EX28DRAFT_3354: EX28DRAFT_3354 - succinate dehydrogenase and fumarate reductase iron-sulfur protein, at 19,614 to 20,348
_3354
EX28DRAFT_3355: EX28DRAFT_3355 - succinate dehydrogenase subunit A (EC 1.3.5.1), at 20,341 to 22,131
_3355
Position (kb) 19
20 Strain fitness (log2 ratio) -1
0
1
2 at 18.220 kb on + strand, within EX28DRAFT_3351 at 18.291 kb on - strand, within EX28DRAFT_3351 at 18.298 kb on + strand, within EX28DRAFT_3351 at 18.298 kb on + strand, within EX28DRAFT_3351 at 18.322 kb on + strand, within EX28DRAFT_3351 at 18.323 kb on - strand, within EX28DRAFT_3351 at 18.359 kb on + strand, within EX28DRAFT_3351 at 18.360 kb on - strand, within EX28DRAFT_3351 at 18.439 kb on + strand, within EX28DRAFT_3351 at 18.450 kb on + strand, within EX28DRAFT_3351 at 18.490 kb on + strand, within EX28DRAFT_3351 at 18.507 kb on - strand, within EX28DRAFT_3351 at 18.507 kb on - strand, within EX28DRAFT_3351 at 18.667 kb on - strand, within EX28DRAFT_3351 at 18.807 kb on - strand at 18.893 kb on + strand, within EX28DRAFT_3352 at 18.893 kb on + strand, within EX28DRAFT_3352 at 18.893 kb on + strand, within EX28DRAFT_3352 at 18.894 kb on - strand, within EX28DRAFT_3352 at 19.083 kb on + strand, within EX28DRAFT_3352 at 19.274 kb on - strand, within EX28DRAFT_3353 at 19.471 kb on - strand, within EX28DRAFT_3353 at 19.528 kb on - strand, within EX28DRAFT_3353 at 19.581 kb on + strand at 19.581 kb on + strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.582 kb on - strand at 19.615 kb on + strand at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.772 kb on + strand, within EX28DRAFT_3354 at 19.773 kb on - strand, within EX28DRAFT_3354 at 19.990 kb on + strand, within EX28DRAFT_3354 at 19.991 kb on - strand, within EX28DRAFT_3354 at 19.998 kb on - strand, within EX28DRAFT_3354 at 20.082 kb on - strand, within EX28DRAFT_3354 at 20.095 kb on + strand, within EX28DRAFT_3354 at 20.114 kb on + strand, within EX28DRAFT_3354 at 20.115 kb on - strand, within EX28DRAFT_3354 at 20.189 kb on - strand, within EX28DRAFT_3354 at 20.269 kb on + strand, within EX28DRAFT_3354 at 20.270 kb on - strand, within EX28DRAFT_3354 at 20.309 kb on - strand at 20.401 kb on - strand at 20.401 kb on - strand at 20.436 kb on - strand at 20.436 kb on - strand at 20.577 kb on + strand, within EX28DRAFT_3355 at 20.577 kb on + strand, within EX28DRAFT_3355 at 20.578 kb on - strand, within EX28DRAFT_3355 at 20.578 kb on - strand, within EX28DRAFT_3355
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 18,220 + EX28DRAFT_3351 0.33 +0.6 18,291 - EX28DRAFT_3351 0.41 +0.2 18,298 + EX28DRAFT_3351 0.42 -0.1 18,298 + EX28DRAFT_3351 0.42 +2.0 18,322 + EX28DRAFT_3351 0.44 -0.4 18,323 - EX28DRAFT_3351 0.45 +0.4 18,359 + EX28DRAFT_3351 0.49 -0.3 18,360 - EX28DRAFT_3351 0.49 -0.4 18,439 + EX28DRAFT_3351 0.58 +0.0 18,450 + EX28DRAFT_3351 0.59 +0.6 18,490 + EX28DRAFT_3351 0.64 +0.5 18,507 - EX28DRAFT_3351 0.66 +0.1 18,507 - EX28DRAFT_3351 0.66 -1.3 18,667 - EX28DRAFT_3351 0.84 -0.5 18,807 - +0.4 18,893 + EX28DRAFT_3352 0.15 -0.1 18,893 + EX28DRAFT_3352 0.15 +0.1 18,893 + EX28DRAFT_3352 0.15 +0.1 18,894 - EX28DRAFT_3352 0.16 -0.2 19,083 + EX28DRAFT_3352 0.68 +0.3 19,274 - EX28DRAFT_3353 0.17 -1.2 19,471 - EX28DRAFT_3353 0.66 -1.0 19,528 - EX28DRAFT_3353 0.81 +0.3 19,581 + -0.1 19,581 + -0.9 19,582 - +0.2 19,582 - +0.0 19,582 - +0.4 19,582 - -0.6 19,615 + +0.0 19,772 + EX28DRAFT_3354 0.21 +0.2 19,772 + EX28DRAFT_3354 0.21 -0.1 19,772 + EX28DRAFT_3354 0.21 +0.5 19,773 - EX28DRAFT_3354 0.22 -1.0 19,990 + EX28DRAFT_3354 0.51 -0.7 19,991 - EX28DRAFT_3354 0.51 -0.6 19,998 - EX28DRAFT_3354 0.52 +0.5 20,082 - EX28DRAFT_3354 0.64 -0.2 20,095 + EX28DRAFT_3354 0.65 -0.4 20,114 + EX28DRAFT_3354 0.68 -1.5 20,115 - EX28DRAFT_3354 0.68 -0.3 20,189 - EX28DRAFT_3354 0.78 +0.1 20,269 + EX28DRAFT_3354 0.89 -0.5 20,270 - EX28DRAFT_3354 0.89 +0.9 20,309 - +0.3 20,401 - +0.5 20,401 - -1.2 20,436 - -0.2 20,436 - -0.5 20,577 + EX28DRAFT_3355 0.13 -0.8 20,577 + EX28DRAFT_3355 0.13 +0.3 20,578 - EX28DRAFT_3355 0.13 -0.2 20,578 - EX28DRAFT_3355 0.13 -1.3
Or see this region's nucleotide sequence