Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2895 and EX28DRAFT_2896 overlap by 4 nucleotides EX28DRAFT_2896 and EX28DRAFT_2897 are separated by 140 nucleotides
EX28DRAFT_2895: EX28DRAFT_2895 - DNA helicase/exodeoxyribonuclease V, beta subunit (EC 3.1.11.5), at 200,253 to 203,795
_2895
EX28DRAFT_2896: EX28DRAFT_2896 - Secreted/periplasmic Zn-dependent peptidases, insulinase-like, at 203,792 to 206,659
_2896
EX28DRAFT_2897: EX28DRAFT_2897 - DNA helicase/exodeoxyribonuclease V, gamma subunit (EC 3.1.11.5), at 206,800 to 210,174
_2897
Position (kb)
203
204
205
206
207 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 203.758 kb on - strand at 203.763 kb on + strand at 203.764 kb on - strand at 203.815 kb on + strand at 203.816 kb on - strand at 203.816 kb on - strand at 203.871 kb on + strand at 203.932 kb on + strand at 203.933 kb on - strand at 203.997 kb on - strand at 204.251 kb on + strand, within EX28DRAFT_2896 at 204.251 kb on + strand, within EX28DRAFT_2896 at 204.252 kb on - strand, within EX28DRAFT_2896 at 204.252 kb on - strand, within EX28DRAFT_2896 at 204.312 kb on + strand, within EX28DRAFT_2896 at 204.312 kb on + strand, within EX28DRAFT_2896 at 204.313 kb on - strand, within EX28DRAFT_2896 at 204.313 kb on - strand, within EX28DRAFT_2896 at 204.669 kb on + strand, within EX28DRAFT_2896 at 204.670 kb on - strand, within EX28DRAFT_2896 at 204.700 kb on + strand, within EX28DRAFT_2896 at 204.701 kb on - strand, within EX28DRAFT_2896 at 204.754 kb on - strand, within EX28DRAFT_2896 at 204.795 kb on + strand, within EX28DRAFT_2896 at 204.796 kb on - strand, within EX28DRAFT_2896 at 204.847 kb on + strand, within EX28DRAFT_2896 at 204.954 kb on + strand, within EX28DRAFT_2896 at 205.069 kb on - strand, within EX28DRAFT_2896 at 205.069 kb on - strand, within EX28DRAFT_2896 at 205.157 kb on - strand, within EX28DRAFT_2896 at 205.158 kb on + strand, within EX28DRAFT_2896 at 205.158 kb on + strand, within EX28DRAFT_2896 at 205.158 kb on + strand, within EX28DRAFT_2896 at 205.159 kb on - strand, within EX28DRAFT_2896 at 205.159 kb on - strand, within EX28DRAFT_2896 at 205.267 kb on - strand, within EX28DRAFT_2896 at 205.279 kb on + strand, within EX28DRAFT_2896 at 205.302 kb on + strand, within EX28DRAFT_2896 at 205.302 kb on + strand, within EX28DRAFT_2896 at 205.303 kb on - strand, within EX28DRAFT_2896 at 205.378 kb on + strand, within EX28DRAFT_2896 at 205.444 kb on - strand, within EX28DRAFT_2896 at 205.551 kb on + strand, within EX28DRAFT_2896 at 205.669 kb on + strand, within EX28DRAFT_2896 at 205.730 kb on + strand, within EX28DRAFT_2896 at 205.730 kb on + strand, within EX28DRAFT_2896 at 205.731 kb on - strand, within EX28DRAFT_2896 at 205.834 kb on - strand, within EX28DRAFT_2896 at 205.834 kb on - strand, within EX28DRAFT_2896 at 205.834 kb on - strand, within EX28DRAFT_2896 at 205.927 kb on + strand, within EX28DRAFT_2896 at 205.998 kb on + strand, within EX28DRAFT_2896 at 205.998 kb on + strand, within EX28DRAFT_2896 at 206.001 kb on + strand, within EX28DRAFT_2896 at 206.001 kb on + strand, within EX28DRAFT_2896 at 206.002 kb on - strand, within EX28DRAFT_2896 at 206.002 kb on - strand, within EX28DRAFT_2896 at 206.203 kb on - strand, within EX28DRAFT_2896 at 206.203 kb on - strand, within EX28DRAFT_2896 at 206.287 kb on - strand, within EX28DRAFT_2896 at 206.398 kb on + strand at 206.398 kb on + strand at 206.398 kb on + strand at 206.399 kb on - strand at 206.399 kb on - strand at 206.399 kb on - strand at 206.410 kb on - strand at 206.570 kb on - strand at 206.673 kb on + strand at 206.674 kb on - strand at 206.683 kb on - strand at 206.732 kb on + strand at 206.732 kb on + strand at 206.761 kb on + strand at 206.777 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 203,758 - +0.4 203,763 + -0.3 203,764 - -0.0 203,815 + +1.2 203,816 - +2.2 203,816 - +0.1 203,871 + -0.8 203,932 + +0.2 203,933 - +0.3 203,997 - +3.2 204,251 + EX28DRAFT_2896 0.16 -0.5 204,251 + EX28DRAFT_2896 0.16 -0.3 204,252 - EX28DRAFT_2896 0.16 +0.8 204,252 - EX28DRAFT_2896 0.16 -0.5 204,312 + EX28DRAFT_2896 0.18 -0.3 204,312 + EX28DRAFT_2896 0.18 +0.4 204,313 - EX28DRAFT_2896 0.18 +1.1 204,313 - EX28DRAFT_2896 0.18 +0.1 204,669 + EX28DRAFT_2896 0.31 -0.2 204,670 - EX28DRAFT_2896 0.31 -0.1 204,700 + EX28DRAFT_2896 0.32 +0.3 204,701 - EX28DRAFT_2896 0.32 -0.3 204,754 - EX28DRAFT_2896 0.34 +0.5 204,795 + EX28DRAFT_2896 0.35 -0.7 204,796 - EX28DRAFT_2896 0.35 +0.1 204,847 + EX28DRAFT_2896 0.37 -1.3 204,954 + EX28DRAFT_2896 0.41 -1.0 205,069 - EX28DRAFT_2896 0.45 -1.9 205,069 - EX28DRAFT_2896 0.45 +0.1 205,157 - EX28DRAFT_2896 0.48 -1.3 205,158 + EX28DRAFT_2896 0.48 -1.9 205,158 + EX28DRAFT_2896 0.48 -0.8 205,158 + EX28DRAFT_2896 0.48 +0.0 205,159 - EX28DRAFT_2896 0.48 -0.0 205,159 - EX28DRAFT_2896 0.48 -0.8 205,267 - EX28DRAFT_2896 0.51 -2.1 205,279 + EX28DRAFT_2896 0.52 -1.5 205,302 + EX28DRAFT_2896 0.53 -0.8 205,302 + EX28DRAFT_2896 0.53 -1.7 205,303 - EX28DRAFT_2896 0.53 +0.5 205,378 + EX28DRAFT_2896 0.55 -1.2 205,444 - EX28DRAFT_2896 0.58 +0.7 205,551 + EX28DRAFT_2896 0.61 -0.3 205,669 + EX28DRAFT_2896 0.65 +0.4 205,730 + EX28DRAFT_2896 0.68 -0.5 205,730 + EX28DRAFT_2896 0.68 -0.7 205,731 - EX28DRAFT_2896 0.68 +0.3 205,834 - EX28DRAFT_2896 0.71 -0.2 205,834 - EX28DRAFT_2896 0.71 +1.3 205,834 - EX28DRAFT_2896 0.71 +0.3 205,927 + EX28DRAFT_2896 0.74 +0.4 205,998 + EX28DRAFT_2896 0.77 +0.7 205,998 + EX28DRAFT_2896 0.77 -1.5 206,001 + EX28DRAFT_2896 0.77 -0.2 206,001 + EX28DRAFT_2896 0.77 -0.0 206,002 - EX28DRAFT_2896 0.77 +1.7 206,002 - EX28DRAFT_2896 0.77 -2.2 206,203 - EX28DRAFT_2896 0.84 +0.1 206,203 - EX28DRAFT_2896 0.84 +0.7 206,287 - EX28DRAFT_2896 0.87 +0.4 206,398 + +0.1 206,398 + +0.1 206,398 + -0.3 206,399 - +1.0 206,399 - +0.2 206,399 - -0.2 206,410 - +0.1 206,570 - -0.0 206,673 + -1.7 206,674 - +0.7 206,683 - -3.7 206,732 + +0.3 206,732 + -0.4 206,761 + -0.2 206,777 - -0.5
Or see this region's nucleotide sequence