Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2853 and EX28DRAFT_2854 are separated by 306 nucleotides EX28DRAFT_2854 and EX28DRAFT_2855 are separated by 85 nucleotides EX28DRAFT_2855 and EX28DRAFT_2856 are separated by 54 nucleotides
EX28DRAFT_2853: EX28DRAFT_2853 - sulfite reductase [NADPH] flavoprotein, alpha-component, at 154,670 to 156,475
_2853
EX28DRAFT_2854: EX28DRAFT_2854 - queuosine biosynthesis protein QueD, at 156,782 to 157,144
_2854
EX28DRAFT_2855: EX28DRAFT_2855 - putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE, at 157,230 to 157,901
_2855
EX28DRAFT_2856: EX28DRAFT_2856 - Putative N-acetylmannosamine-6-phosphate epimerase, at 157,956 to 158,663
_2856
Position (kb) 156
157
158 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 156.003 kb on + strand, within EX28DRAFT_2853 at 156.173 kb on - strand, within EX28DRAFT_2853 at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.555 kb on - strand at 156.561 kb on + strand at 156.561 kb on + strand at 156.578 kb on + strand at 156.585 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.617 kb on + strand at 156.618 kb on - strand at 156.718 kb on + strand at 156.746 kb on + strand at 156.746 kb on + strand at 156.853 kb on + strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.974 kb on + strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 157.127 kb on - strand at 157.127 kb on - strand at 157.130 kb on + strand at 157.239 kb on - strand at 157.338 kb on + strand, within EX28DRAFT_2855 at 157.427 kb on + strand, within EX28DRAFT_2855 at 157.461 kb on + strand, within EX28DRAFT_2855 at 157.462 kb on - strand, within EX28DRAFT_2855 at 157.731 kb on + strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.737 kb on - strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.775 kb on + strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.776 kb on - strand, within EX28DRAFT_2855 at 157.818 kb on + strand, within EX28DRAFT_2855 at 157.852 kb on + strand at 157.852 kb on + strand at 157.852 kb on + strand at 157.902 kb on - strand at 157.931 kb on - strand at 157.934 kb on - strand at 157.994 kb on + strand at 157.995 kb on - strand at 158.007 kb on - strand at 158.049 kb on - strand, within EX28DRAFT_2856
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 156,003 + EX28DRAFT_2853 0.74 -0.2 156,173 - EX28DRAFT_2853 0.83 +0.0 156,533 + -0.5 156,533 + -1.4 156,534 - -0.6 156,555 - -0.3 156,561 + +0.5 156,561 + -0.6 156,578 + +1.0 156,585 + -0.5 156,608 + -1.7 156,609 - +0.2 156,617 + -0.3 156,618 - +0.1 156,718 + +0.6 156,746 + -0.6 156,746 + +1.0 156,853 + EX28DRAFT_2854 0.20 +0.1 156,854 - EX28DRAFT_2854 0.20 -0.1 156,854 - EX28DRAFT_2854 0.20 +0.1 156,974 + EX28DRAFT_2854 0.53 -0.3 156,975 - EX28DRAFT_2854 0.53 -0.4 156,975 - EX28DRAFT_2854 0.53 -0.9 157,127 - +0.2 157,127 - +0.4 157,130 + -0.2 157,239 - +0.4 157,338 + EX28DRAFT_2855 0.16 +0.3 157,427 + EX28DRAFT_2855 0.29 -3.4 157,461 + EX28DRAFT_2855 0.34 -0.5 157,462 - EX28DRAFT_2855 0.35 +0.2 157,731 + EX28DRAFT_2855 0.75 -0.5 157,737 - EX28DRAFT_2855 0.75 -0.3 157,737 - EX28DRAFT_2855 0.75 -0.9 157,737 - EX28DRAFT_2855 0.75 +0.5 157,775 + EX28DRAFT_2855 0.81 +0.0 157,775 + EX28DRAFT_2855 0.81 -1.3 157,775 + EX28DRAFT_2855 0.81 +0.0 157,776 - EX28DRAFT_2855 0.81 +1.0 157,776 - EX28DRAFT_2855 0.81 +1.4 157,818 + EX28DRAFT_2855 0.88 -0.3 157,852 + -0.8 157,852 + +0.8 157,852 + -0.1 157,902 - -1.0 157,931 - -1.2 157,934 - -0.4 157,994 + +0.1 157,995 - +0.1 158,007 - +0.9 158,049 - EX28DRAFT_2856 0.13 -0.5
Or see this region's nucleotide sequence