Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2832 and EX28DRAFT_2833 are separated by 171 nucleotides EX28DRAFT_2833 and EX28DRAFT_2834 overlap by 4 nucleotides EX28DRAFT_2834 and EX28DRAFT_2835 are separated by 105 nucleotides
EX28DRAFT_2832: EX28DRAFT_2832 - polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases, at 135,964 to 136,566
_2832
EX28DRAFT_2833: EX28DRAFT_2833 - Transcriptional regulators, at 136,738 to 137,145
_2833
EX28DRAFT_2834: EX28DRAFT_2834 - Transcriptional regulator, at 137,142 to 138,032
_2834
EX28DRAFT_2835: EX28DRAFT_2835 - Arabinose efflux permease, at 138,138 to 139,340
_2835
Position (kb) 137
138
139 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 136.356 kb on + strand, within EX28DRAFT_2832 at 136.556 kb on - strand at 136.607 kb on + strand at 136.607 kb on + strand at 136.607 kb on + strand at 136.608 kb on - strand at 136.608 kb on - strand at 136.608 kb on - strand at 136.612 kb on + strand at 136.908 kb on + strand, within EX28DRAFT_2833 at 137.144 kb on - strand at 137.144 kb on - strand at 137.144 kb on - strand at 137.144 kb on - strand at 137.221 kb on + strand at 137.221 kb on + strand at 137.243 kb on + strand, within EX28DRAFT_2834 at 137.244 kb on - strand, within EX28DRAFT_2834 at 137.245 kb on + strand, within EX28DRAFT_2834 at 137.245 kb on + strand, within EX28DRAFT_2834 at 137.245 kb on + strand, within EX28DRAFT_2834 at 137.246 kb on - strand, within EX28DRAFT_2834 at 137.463 kb on - strand, within EX28DRAFT_2834 at 137.628 kb on - strand, within EX28DRAFT_2834 at 137.628 kb on - strand, within EX28DRAFT_2834 at 137.725 kb on + strand, within EX28DRAFT_2834 at 137.911 kb on - strand, within EX28DRAFT_2834 at 137.922 kb on - strand, within EX28DRAFT_2834 at 138.024 kb on - strand at 138.107 kb on + strand at 138.108 kb on - strand at 138.112 kb on + strand at 138.144 kb on + strand at 138.168 kb on + strand at 138.200 kb on + strand at 138.200 kb on + strand at 138.200 kb on + strand at 138.201 kb on - strand at 138.201 kb on - strand at 138.207 kb on - strand at 138.286 kb on - strand, within EX28DRAFT_2835 at 138.467 kb on - strand, within EX28DRAFT_2835 at 138.607 kb on + strand, within EX28DRAFT_2835 at 138.608 kb on - strand, within EX28DRAFT_2835 at 138.768 kb on + strand, within EX28DRAFT_2835 at 138.768 kb on + strand, within EX28DRAFT_2835 at 138.768 kb on + strand, within EX28DRAFT_2835 at 138.769 kb on - strand, within EX28DRAFT_2835 at 138.769 kb on - strand, within EX28DRAFT_2835 at 138.769 kb on - strand, within EX28DRAFT_2835 at 138.769 kb on - strand, within EX28DRAFT_2835 at 138.770 kb on + strand, within EX28DRAFT_2835 at 138.770 kb on + strand, within EX28DRAFT_2835 at 138.819 kb on + strand, within EX28DRAFT_2835 at 138.820 kb on - strand, within EX28DRAFT_2835 at 138.820 kb on - strand, within EX28DRAFT_2835 at 138.924 kb on - strand, within EX28DRAFT_2835
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 136,356 + EX28DRAFT_2832 0.65 -1.0 136,556 - +0.6 136,607 + +0.1 136,607 + -1.1 136,607 + -1.0 136,608 - +0.3 136,608 - -0.2 136,608 - -0.4 136,612 + -1.4 136,908 + EX28DRAFT_2833 0.42 -2.5 137,144 - -0.1 137,144 - -2.0 137,144 - +0.4 137,144 - +0.6 137,221 + -0.2 137,221 + -1.7 137,243 + EX28DRAFT_2834 0.11 +0.7 137,244 - EX28DRAFT_2834 0.11 +0.3 137,245 + EX28DRAFT_2834 0.12 +0.1 137,245 + EX28DRAFT_2834 0.12 -0.0 137,245 + EX28DRAFT_2834 0.12 +0.4 137,246 - EX28DRAFT_2834 0.12 -0.8 137,463 - EX28DRAFT_2834 0.36 +0.8 137,628 - EX28DRAFT_2834 0.55 +1.5 137,628 - EX28DRAFT_2834 0.55 +0.1 137,725 + EX28DRAFT_2834 0.65 +0.2 137,911 - EX28DRAFT_2834 0.86 +0.1 137,922 - EX28DRAFT_2834 0.88 -0.4 138,024 - -1.1 138,107 + -0.6 138,108 - -1.2 138,112 + +0.0 138,144 + -0.4 138,168 + -0.0 138,200 + -0.1 138,200 + -2.4 138,200 + +0.4 138,201 - -0.1 138,201 - -0.3 138,207 - +0.6 138,286 - EX28DRAFT_2835 0.12 -0.5 138,467 - EX28DRAFT_2835 0.27 -0.3 138,607 + EX28DRAFT_2835 0.39 +1.1 138,608 - EX28DRAFT_2835 0.39 +0.1 138,768 + EX28DRAFT_2835 0.52 -0.2 138,768 + EX28DRAFT_2835 0.52 +0.1 138,768 + EX28DRAFT_2835 0.52 +0.2 138,769 - EX28DRAFT_2835 0.52 +0.1 138,769 - EX28DRAFT_2835 0.52 -1.2 138,769 - EX28DRAFT_2835 0.52 -0.4 138,769 - EX28DRAFT_2835 0.52 -0.2 138,770 + EX28DRAFT_2835 0.53 -0.1 138,770 + EX28DRAFT_2835 0.53 -0.2 138,819 + EX28DRAFT_2835 0.57 +0.6 138,820 - EX28DRAFT_2835 0.57 -2.8 138,820 - EX28DRAFT_2835 0.57 -0.1 138,924 - EX28DRAFT_2835 0.65 +0.0
Or see this region's nucleotide sequence