Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2755 and EX28DRAFT_2756 are separated by 495 nucleotides EX28DRAFT_2756 and EX28DRAFT_2757 are separated by 32 nucleotides EX28DRAFT_2757 and EX28DRAFT_2758 are separated by 160 nucleotides EX28DRAFT_2758 and EX28DRAFT_2759 are separated by 243 nucleotides EX28DRAFT_2759 and EX28DRAFT_2760 overlap by 4 nucleotides
EX28DRAFT_2755: EX28DRAFT_2755 - DNA-binding protein H-NS, at 63,706 to 64,110
_2755
EX28DRAFT_2756: EX28DRAFT_2756 - Protein of unknown function (DUF1144), at 64,606 to 65,055
_2756
EX28DRAFT_2757: EX28DRAFT_2757 - Protein of unknown function (DUF2002), at 65,088 to 65,432
_2757
EX28DRAFT_2758: EX28DRAFT_2758 - Uncharacterized conserved protein, at 65,593 to 65,919
_2758
EX28DRAFT_2759: EX28DRAFT_2759 - ribonucleoside-diphosphate reductase class Ib glutaredoxin subunit (EC 1.17.4.1), at 66,163 to 66,402
_2759
EX28DRAFT_2760: EX28DRAFT_2760 - ribonucleoside-diphosphate reductase 2, operon protein nrdI, at 66,399 to 66,809
_2760
Position (kb) 65
66 Strain fitness (log2 ratio) -2
-1
0
1 at 64.114 kb on - strand at 64.114 kb on - strand at 64.144 kb on - strand at 64.147 kb on + strand at 64.148 kb on - strand at 64.182 kb on + strand at 64.214 kb on + strand at 64.215 kb on - strand at 64.218 kb on + strand at 64.221 kb on - strand at 64.294 kb on - strand at 64.500 kb on - strand at 64.519 kb on - strand at 64.525 kb on + strand at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.697 kb on + strand, within EX28DRAFT_2756 at 64.762 kb on - strand, within EX28DRAFT_2756 at 64.765 kb on - strand, within EX28DRAFT_2756 at 64.781 kb on - strand, within EX28DRAFT_2756 at 64.867 kb on + strand, within EX28DRAFT_2756 at 64.868 kb on - strand, within EX28DRAFT_2756 at 65.023 kb on - strand at 65.035 kb on + strand at 65.090 kb on - strand at 65.094 kb on + strand at 65.094 kb on + strand at 65.095 kb on - strand at 65.165 kb on - strand, within EX28DRAFT_2757 at 65.323 kb on + strand, within EX28DRAFT_2757 at 65.324 kb on - strand, within EX28DRAFT_2757 at 65.324 kb on - strand, within EX28DRAFT_2757 at 65.324 kb on - strand, within EX28DRAFT_2757 at 65.329 kb on + strand, within EX28DRAFT_2757 at 65.329 kb on + strand, within EX28DRAFT_2757 at 65.329 kb on + strand, within EX28DRAFT_2757 at 65.353 kb on + strand, within EX28DRAFT_2757 at 65.428 kb on + strand at 65.428 kb on + strand at 65.428 kb on + strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.429 kb on - strand at 65.433 kb on - strand at 65.469 kb on + strand at 65.469 kb on + strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.470 kb on - strand at 65.474 kb on + strand at 65.485 kb on - strand at 65.485 kb on - strand at 65.501 kb on + strand at 65.541 kb on + strand at 65.541 kb on + strand at 65.545 kb on - strand at 65.620 kb on - strand at 65.853 kb on - strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.856 kb on + strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.857 kb on - strand, within EX28DRAFT_2758 at 65.918 kb on - strand at 66.033 kb on - strand at 66.078 kb on - strand at 66.078 kb on - strand at 66.104 kb on - strand at 66.104 kb on - strand at 66.399 kb on - strand at 66.414 kb on + strand at 66.414 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 64,114 - +0.3 64,114 - -0.1 64,144 - -0.2 64,147 + +0.8 64,148 - -0.0 64,182 + -0.1 64,214 + -1.4 64,215 - +0.5 64,218 + -0.1 64,221 - -0.7 64,294 - +0.7 64,500 - +0.4 64,519 - -0.1 64,525 + -1.0 64,697 + EX28DRAFT_2756 0.20 -0.5 64,697 + EX28DRAFT_2756 0.20 +1.6 64,762 - EX28DRAFT_2756 0.35 -0.8 64,765 - EX28DRAFT_2756 0.35 +0.8 64,781 - EX28DRAFT_2756 0.39 +0.2 64,867 + EX28DRAFT_2756 0.58 +0.3 64,868 - EX28DRAFT_2756 0.58 +0.2 65,023 - +0.2 65,035 + +0.4 65,090 - -0.3 65,094 + -0.7 65,094 + -2.2 65,095 - -2.0 65,165 - EX28DRAFT_2757 0.22 -0.6 65,323 + EX28DRAFT_2757 0.68 -0.0 65,324 - EX28DRAFT_2757 0.68 -2.0 65,324 - EX28DRAFT_2757 0.68 -0.5 65,324 - EX28DRAFT_2757 0.68 -0.2 65,329 + EX28DRAFT_2757 0.70 +0.4 65,329 + EX28DRAFT_2757 0.70 +0.3 65,329 + EX28DRAFT_2757 0.70 -0.2 65,353 + EX28DRAFT_2757 0.77 -1.7 65,428 + +0.3 65,428 + -0.4 65,428 + -0.1 65,429 - +1.2 65,429 - +1.2 65,429 - +0.0 65,433 - -0.2 65,469 + +0.2 65,469 + -0.6 65,470 - +0.0 65,470 - -0.7 65,470 - -0.0 65,474 + -1.4 65,485 - -0.3 65,485 - +0.2 65,501 + +1.5 65,541 + -0.0 65,541 + +0.6 65,545 - -0.6 65,620 - -0.2 65,853 - EX28DRAFT_2758 0.80 +0.3 65,856 + EX28DRAFT_2758 0.80 -0.8 65,856 + EX28DRAFT_2758 0.80 +0.9 65,856 + EX28DRAFT_2758 0.80 -0.4 65,857 - EX28DRAFT_2758 0.81 +1.0 65,857 - EX28DRAFT_2758 0.81 -0.1 65,918 - -1.4 66,033 - +0.1 66,078 - +0.6 66,078 - +0.3 66,104 - -0.4 66,104 - -2.3 66,399 - +0.2 66,414 + -0.4 66,414 + -0.9
Or see this region's nucleotide sequence