Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2643 and EX28DRAFT_2644 overlap by 4 nucleotides EX28DRAFT_2644 and EX28DRAFT_2645 are separated by 18 nucleotides
EX28DRAFT_2643: EX28DRAFT_2643 - hypothetical protein, at 2,681,419 to 2,682,153
_2643
EX28DRAFT_2644: EX28DRAFT_2644 - Ogr/Delta-like zinc finger, at 2,682,150 to 2,682,377
_2644
EX28DRAFT_2645: EX28DRAFT_2645 - Phage polarity suppression protein (Psu), at 2,682,396 to 2,682,962
_2645
Position (kb)
2682
2683 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2681.239 kb on - strand at 2681.245 kb on + strand at 2681.251 kb on + strand at 2681.251 kb on + strand at 2681.251 kb on + strand at 2681.252 kb on - strand at 2681.252 kb on - strand at 2681.252 kb on - strand at 2681.350 kb on + strand at 2681.368 kb on + strand at 2681.368 kb on + strand at 2681.413 kb on - strand at 2681.443 kb on + strand at 2681.529 kb on + strand, within EX28DRAFT_2643 at 2681.529 kb on + strand, within EX28DRAFT_2643 at 2681.529 kb on + strand, within EX28DRAFT_2643 at 2681.663 kb on + strand, within EX28DRAFT_2643 at 2681.663 kb on + strand, within EX28DRAFT_2643 at 2681.682 kb on - strand, within EX28DRAFT_2643 at 2681.832 kb on + strand, within EX28DRAFT_2643 at 2681.833 kb on - strand, within EX28DRAFT_2643 at 2681.948 kb on - strand, within EX28DRAFT_2643 at 2681.951 kb on + strand, within EX28DRAFT_2643 at 2681.951 kb on + strand, within EX28DRAFT_2643 at 2681.951 kb on + strand, within EX28DRAFT_2643 at 2681.951 kb on + strand, within EX28DRAFT_2643 at 2681.951 kb on + strand, within EX28DRAFT_2643 at 2681.952 kb on - strand, within EX28DRAFT_2643 at 2681.989 kb on - strand, within EX28DRAFT_2643 at 2682.052 kb on - strand, within EX28DRAFT_2643 at 2682.052 kb on - strand, within EX28DRAFT_2643 at 2682.095 kb on - strand at 2682.192 kb on - strand, within EX28DRAFT_2644 at 2682.229 kb on - strand, within EX28DRAFT_2644 at 2682.245 kb on + strand, within EX28DRAFT_2644 at 2682.246 kb on - strand, within EX28DRAFT_2644 at 2682.295 kb on + strand, within EX28DRAFT_2644 at 2682.585 kb on - strand, within EX28DRAFT_2645 at 2682.640 kb on + strand, within EX28DRAFT_2645 at 2682.641 kb on - strand, within EX28DRAFT_2645 at 2682.641 kb on - strand, within EX28DRAFT_2645 at 2682.641 kb on - strand, within EX28DRAFT_2645 at 2682.641 kb on - strand, within EX28DRAFT_2645 at 2682.641 kb on - strand, within EX28DRAFT_2645 at 2682.644 kb on + strand, within EX28DRAFT_2645 at 2682.719 kb on + strand, within EX28DRAFT_2645 at 2682.720 kb on - strand, within EX28DRAFT_2645 at 2682.739 kb on + strand, within EX28DRAFT_2645 at 2682.771 kb on + strand, within EX28DRAFT_2645 at 2683.004 kb on + strand at 2683.020 kb on - strand at 2683.020 kb on - strand at 2683.045 kb on + strand at 2683.111 kb on + strand at 2683.218 kb on - strand at 2683.297 kb on - strand at 2683.353 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,681,239 - -1.0 2,681,245 + -0.3 2,681,251 + +1.6 2,681,251 + +0.3 2,681,251 + +0.7 2,681,252 - -1.1 2,681,252 - +1.1 2,681,252 - -0.6 2,681,350 + +0.2 2,681,368 + -0.4 2,681,368 + -0.6 2,681,413 - -0.5 2,681,443 + +0.5 2,681,529 + EX28DRAFT_2643 0.15 +0.4 2,681,529 + EX28DRAFT_2643 0.15 +2.4 2,681,529 + EX28DRAFT_2643 0.15 -0.5 2,681,663 + EX28DRAFT_2643 0.33 +0.1 2,681,663 + EX28DRAFT_2643 0.33 +0.6 2,681,682 - EX28DRAFT_2643 0.36 -0.6 2,681,832 + EX28DRAFT_2643 0.56 +1.1 2,681,833 - EX28DRAFT_2643 0.56 +0.1 2,681,948 - EX28DRAFT_2643 0.72 +1.1 2,681,951 + EX28DRAFT_2643 0.72 +0.6 2,681,951 + EX28DRAFT_2643 0.72 +1.7 2,681,951 + EX28DRAFT_2643 0.72 +0.2 2,681,951 + EX28DRAFT_2643 0.72 -1.4 2,681,951 + EX28DRAFT_2643 0.72 -0.6 2,681,952 - EX28DRAFT_2643 0.73 +0.4 2,681,989 - EX28DRAFT_2643 0.78 +0.4 2,682,052 - EX28DRAFT_2643 0.86 -0.3 2,682,052 - EX28DRAFT_2643 0.86 -0.1 2,682,095 - -2.6 2,682,192 - EX28DRAFT_2644 0.18 -2.4 2,682,229 - EX28DRAFT_2644 0.35 +0.3 2,682,245 + EX28DRAFT_2644 0.42 -0.6 2,682,246 - EX28DRAFT_2644 0.42 -1.1 2,682,295 + EX28DRAFT_2644 0.64 -0.3 2,682,585 - EX28DRAFT_2645 0.33 +0.5 2,682,640 + EX28DRAFT_2645 0.43 -0.5 2,682,641 - EX28DRAFT_2645 0.43 -1.1 2,682,641 - EX28DRAFT_2645 0.43 -0.1 2,682,641 - EX28DRAFT_2645 0.43 -0.4 2,682,641 - EX28DRAFT_2645 0.43 +0.7 2,682,641 - EX28DRAFT_2645 0.43 -0.6 2,682,644 + EX28DRAFT_2645 0.44 +0.5 2,682,719 + EX28DRAFT_2645 0.57 -0.2 2,682,720 - EX28DRAFT_2645 0.57 -0.4 2,682,739 + EX28DRAFT_2645 0.60 +0.1 2,682,771 + EX28DRAFT_2645 0.66 +0.6 2,683,004 + -0.4 2,683,020 - -0.2 2,683,020 - -0.1 2,683,045 + +0.0 2,683,111 + -0.1 2,683,218 - -0.6 2,683,297 - +1.0 2,683,353 - -2.9
Or see this region's nucleotide sequence