Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2421 and EX28DRAFT_2422 are separated by 82 nucleotides EX28DRAFT_2422 and EX28DRAFT_2423 overlap by 1 nucleotides EX28DRAFT_2423 and EX28DRAFT_2424 are separated by 226 nucleotides EX28DRAFT_2424 and EX28DRAFT_2425 are separated by 19 nucleotides
EX28DRAFT_2421: EX28DRAFT_2421 - Protein of unknown function (DUF1158), at 2,448,755 to 2,449,003
_2421
EX28DRAFT_2422: EX28DRAFT_2422 - Helix-turn-helix domain, at 2,449,086 to 2,449,460
_2422
EX28DRAFT_2423: EX28DRAFT_2423 - Predicted Zn-dependent hydrolases of the beta-lactamase fold, at 2,449,460 to 2,450,527
_2423
EX28DRAFT_2424: EX28DRAFT_2424 - transcriptional regulator, TetR family, at 2,450,754 to 2,451,344
_2424
EX28DRAFT_2425: EX28DRAFT_2425 - Uncharacterized protein conserved in bacteria, at 2,451,364 to 2,451,702
_2425
Position (kb)
2449
2450
2451 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2448.561 kb on - strand at 2448.619 kb on + strand at 2448.659 kb on + strand at 2448.798 kb on + strand, within EX28DRAFT_2421 at 2448.906 kb on - strand, within EX28DRAFT_2421 at 2449.268 kb on - strand, within EX28DRAFT_2422 at 2449.298 kb on + strand, within EX28DRAFT_2422 at 2449.353 kb on + strand, within EX28DRAFT_2422 at 2449.353 kb on + strand, within EX28DRAFT_2422 at 2449.353 kb on + strand, within EX28DRAFT_2422 at 2449.354 kb on - strand, within EX28DRAFT_2422 at 2449.355 kb on + strand, within EX28DRAFT_2422 at 2449.355 kb on + strand, within EX28DRAFT_2422 at 2449.355 kb on + strand, within EX28DRAFT_2422 at 2449.355 kb on + strand, within EX28DRAFT_2422 at 2449.356 kb on - strand, within EX28DRAFT_2422 at 2449.356 kb on - strand, within EX28DRAFT_2422 at 2449.360 kb on - strand, within EX28DRAFT_2422 at 2449.462 kb on - strand at 2449.462 kb on - strand at 2449.591 kb on + strand, within EX28DRAFT_2423 at 2449.592 kb on - strand, within EX28DRAFT_2423 at 2449.692 kb on + strand, within EX28DRAFT_2423 at 2449.710 kb on + strand, within EX28DRAFT_2423 at 2449.801 kb on - strand, within EX28DRAFT_2423 at 2449.801 kb on - strand, within EX28DRAFT_2423 at 2449.801 kb on - strand, within EX28DRAFT_2423 at 2449.978 kb on - strand, within EX28DRAFT_2423 at 2450.067 kb on + strand, within EX28DRAFT_2423 at 2450.067 kb on + strand, within EX28DRAFT_2423 at 2450.084 kb on + strand, within EX28DRAFT_2423 at 2450.172 kb on + strand, within EX28DRAFT_2423 at 2450.173 kb on - strand, within EX28DRAFT_2423 at 2450.223 kb on + strand, within EX28DRAFT_2423 at 2450.380 kb on + strand, within EX28DRAFT_2423 at 2450.382 kb on + strand, within EX28DRAFT_2423 at 2450.402 kb on - strand, within EX28DRAFT_2423 at 2450.491 kb on + strand at 2450.502 kb on - strand at 2450.522 kb on - strand at 2450.645 kb on - strand at 2450.652 kb on + strand at 2450.730 kb on - strand at 2451.005 kb on + strand, within EX28DRAFT_2424 at 2451.027 kb on + strand, within EX28DRAFT_2424 at 2451.027 kb on + strand, within EX28DRAFT_2424 at 2451.027 kb on + strand, within EX28DRAFT_2424 at 2451.028 kb on - strand, within EX28DRAFT_2424 at 2451.047 kb on + strand, within EX28DRAFT_2424 at 2451.175 kb on + strand, within EX28DRAFT_2424 at 2451.176 kb on - strand, within EX28DRAFT_2424 at 2451.179 kb on + strand, within EX28DRAFT_2424 at 2451.342 kb on + strand at 2451.342 kb on + strand at 2451.432 kb on + strand, within EX28DRAFT_2425
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,448,561 - -0.8 2,448,619 + -0.6 2,448,659 + +0.6 2,448,798 + EX28DRAFT_2421 0.17 +1.6 2,448,906 - EX28DRAFT_2421 0.61 +1.7 2,449,268 - EX28DRAFT_2422 0.49 -0.2 2,449,298 + EX28DRAFT_2422 0.57 +0.1 2,449,353 + EX28DRAFT_2422 0.71 +0.2 2,449,353 + EX28DRAFT_2422 0.71 +0.6 2,449,353 + EX28DRAFT_2422 0.71 -0.6 2,449,354 - EX28DRAFT_2422 0.71 -0.5 2,449,355 + EX28DRAFT_2422 0.72 -1.3 2,449,355 + EX28DRAFT_2422 0.72 +3.2 2,449,355 + EX28DRAFT_2422 0.72 -2.0 2,449,355 + EX28DRAFT_2422 0.72 +0.1 2,449,356 - EX28DRAFT_2422 0.72 +0.8 2,449,356 - EX28DRAFT_2422 0.72 +0.5 2,449,360 - EX28DRAFT_2422 0.73 +0.4 2,449,462 - +2.1 2,449,462 - +0.4 2,449,591 + EX28DRAFT_2423 0.12 -1.1 2,449,592 - EX28DRAFT_2423 0.12 +0.2 2,449,692 + EX28DRAFT_2423 0.22 -0.4 2,449,710 + EX28DRAFT_2423 0.23 +0.4 2,449,801 - EX28DRAFT_2423 0.32 -0.7 2,449,801 - EX28DRAFT_2423 0.32 -0.9 2,449,801 - EX28DRAFT_2423 0.32 -0.0 2,449,978 - EX28DRAFT_2423 0.49 -0.2 2,450,067 + EX28DRAFT_2423 0.57 -0.0 2,450,067 + EX28DRAFT_2423 0.57 -0.8 2,450,084 + EX28DRAFT_2423 0.58 -0.5 2,450,172 + EX28DRAFT_2423 0.67 +3.2 2,450,173 - EX28DRAFT_2423 0.67 +0.7 2,450,223 + EX28DRAFT_2423 0.71 -1.0 2,450,380 + EX28DRAFT_2423 0.86 +0.0 2,450,382 + EX28DRAFT_2423 0.86 +1.4 2,450,402 - EX28DRAFT_2423 0.88 -0.1 2,450,491 + +0.2 2,450,502 - -0.2 2,450,522 - +0.8 2,450,645 - +0.5 2,450,652 + -1.0 2,450,730 - -1.0 2,451,005 + EX28DRAFT_2424 0.42 +0.9 2,451,027 + EX28DRAFT_2424 0.46 -1.5 2,451,027 + EX28DRAFT_2424 0.46 -1.4 2,451,027 + EX28DRAFT_2424 0.46 -2.4 2,451,028 - EX28DRAFT_2424 0.46 -1.0 2,451,047 + EX28DRAFT_2424 0.50 -3.2 2,451,175 + EX28DRAFT_2424 0.71 +0.5 2,451,176 - EX28DRAFT_2424 0.71 -1.0 2,451,179 + EX28DRAFT_2424 0.72 +1.6 2,451,342 + +0.2 2,451,342 + -0.6 2,451,432 + EX28DRAFT_2425 0.20 -2.0
Or see this region's nucleotide sequence