Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1966 and EX28DRAFT_1967 are separated by 308 nucleotides EX28DRAFT_1967 and EX28DRAFT_1968 are separated by 75 nucleotides EX28DRAFT_1968 and EX28DRAFT_1969 are separated by 72 nucleotides
EX28DRAFT_1966: EX28DRAFT_1966 - DNA-binding winged-HTH domains, at 1,968,579 to 1,969,334
_1966
EX28DRAFT_1967: EX28DRAFT_1967 - hypothetical protein, at 1,969,643 to 1,970,386
_1967
EX28DRAFT_1968: EX28DRAFT_1968 - CS1 type fimbrial major subunit, at 1,970,462 to 1,970,968
_1968
EX28DRAFT_1969: EX28DRAFT_1969 - hypothetical protein, at 1,971,041 to 1,973,758
_1969
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1968.697 kb on + strand, within EX28DRAFT_1966 at 1968.698 kb on - strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.875 kb on - strand, within EX28DRAFT_1966 at 1969.127 kb on + strand, within EX28DRAFT_1966 at 1969.174 kb on - strand, within EX28DRAFT_1966 at 1969.209 kb on - strand, within EX28DRAFT_1966 at 1969.251 kb on + strand, within EX28DRAFT_1966 at 1969.254 kb on + strand, within EX28DRAFT_1966 at 1969.255 kb on - strand, within EX28DRAFT_1966 at 1969.281 kb on + strand at 1969.281 kb on + strand at 1969.282 kb on - strand at 1969.282 kb on - strand at 1969.658 kb on - strand at 1969.683 kb on - strand at 1969.846 kb on + strand, within EX28DRAFT_1967 at 1970.038 kb on + strand, within EX28DRAFT_1967 at 1970.170 kb on - strand, within EX28DRAFT_1967 at 1970.286 kb on + strand, within EX28DRAFT_1967 at 1970.321 kb on - strand at 1970.370 kb on + strand at 1970.445 kb on + strand at 1970.449 kb on + strand at 1970.458 kb on - strand at 1970.467 kb on + strand at 1970.468 kb on - strand at 1970.477 kb on - strand at 1970.539 kb on + strand, within EX28DRAFT_1968 at 1970.540 kb on - strand, within EX28DRAFT_1968 at 1970.576 kb on - strand, within EX28DRAFT_1968 at 1970.625 kb on - strand, within EX28DRAFT_1968 at 1970.625 kb on - strand, within EX28DRAFT_1968 at 1970.670 kb on + strand, within EX28DRAFT_1968 at 1970.671 kb on - strand, within EX28DRAFT_1968 at 1970.671 kb on - strand, within EX28DRAFT_1968 at 1970.671 kb on - strand, within EX28DRAFT_1968 at 1970.671 kb on - strand, within EX28DRAFT_1968 at 1970.807 kb on + strand, within EX28DRAFT_1968 at 1970.807 kb on + strand, within EX28DRAFT_1968 at 1970.808 kb on - strand, within EX28DRAFT_1968 at 1970.808 kb on - strand, within EX28DRAFT_1968 at 1970.809 kb on + strand, within EX28DRAFT_1968 at 1970.809 kb on + strand, within EX28DRAFT_1968 at 1970.909 kb on + strand, within EX28DRAFT_1968 at 1970.981 kb on + strand at 1970.982 kb on - strand at 1970.982 kb on - strand at 1971.179 kb on + strand at 1971.255 kb on + strand at 1971.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,968,697 + EX28DRAFT_1966 0.16 -0.6 1,968,698 - EX28DRAFT_1966 0.16 -0.9 1,968,874 + EX28DRAFT_1966 0.39 +0.6 1,968,874 + EX28DRAFT_1966 0.39 -0.5 1,968,875 - EX28DRAFT_1966 0.39 -1.1 1,969,127 + EX28DRAFT_1966 0.72 -0.3 1,969,174 - EX28DRAFT_1966 0.79 +0.4 1,969,209 - EX28DRAFT_1966 0.83 +0.0 1,969,251 + EX28DRAFT_1966 0.89 +0.0 1,969,254 + EX28DRAFT_1966 0.89 +0.2 1,969,255 - EX28DRAFT_1966 0.89 +1.2 1,969,281 + -0.9 1,969,281 + +0.9 1,969,282 - +1.4 1,969,282 - -0.7 1,969,658 - -0.2 1,969,683 - -0.7 1,969,846 + EX28DRAFT_1967 0.27 -0.6 1,970,038 + EX28DRAFT_1967 0.53 -0.9 1,970,170 - EX28DRAFT_1967 0.71 -0.2 1,970,286 + EX28DRAFT_1967 0.86 -1.2 1,970,321 - +2.2 1,970,370 + -0.4 1,970,445 + +0.6 1,970,449 + -0.1 1,970,458 - -0.6 1,970,467 + +1.9 1,970,468 - -0.1 1,970,477 - -0.6 1,970,539 + EX28DRAFT_1968 0.15 +0.7 1,970,540 - EX28DRAFT_1968 0.15 -0.4 1,970,576 - EX28DRAFT_1968 0.22 -0.8 1,970,625 - EX28DRAFT_1968 0.32 +0.3 1,970,625 - EX28DRAFT_1968 0.32 +0.2 1,970,670 + EX28DRAFT_1968 0.41 -0.3 1,970,671 - EX28DRAFT_1968 0.41 -1.2 1,970,671 - EX28DRAFT_1968 0.41 +1.8 1,970,671 - EX28DRAFT_1968 0.41 -0.4 1,970,671 - EX28DRAFT_1968 0.41 +1.0 1,970,807 + EX28DRAFT_1968 0.68 -0.0 1,970,807 + EX28DRAFT_1968 0.68 -0.2 1,970,808 - EX28DRAFT_1968 0.68 +0.1 1,970,808 - EX28DRAFT_1968 0.68 +1.0 1,970,809 + EX28DRAFT_1968 0.68 -2.5 1,970,809 + EX28DRAFT_1968 0.68 -0.1 1,970,909 + EX28DRAFT_1968 0.88 -1.2 1,970,981 + -1.5 1,970,982 - -0.7 1,970,982 - -0.4 1,971,179 + +0.4 1,971,255 + -0.3 1,971,255 + -0.1
Or see this region's nucleotide sequence