Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1079 and EX28DRAFT_1080 overlap by 11 nucleotides EX28DRAFT_1080 and EX28DRAFT_1081 are separated by 77 nucleotides EX28DRAFT_1081 and EX28DRAFT_1082 are separated by 620 nucleotides
EX28DRAFT_1079: EX28DRAFT_1079 - Transcriptional regulator, at 1,091,506 to 1,092,450
_1079
EX28DRAFT_1080: EX28DRAFT_1080 - Uncharacterized conserved protein, at 1,092,440 to 1,092,880
_1080
EX28DRAFT_1081: EX28DRAFT_1081 - Outer membrane protein (porin), at 1,092,958 to 1,094,040
_1081
EX28DRAFT_1082: EX28DRAFT_1082 - Permeases of the drug/metabolite transporter (DMT) superfamily, at 1,094,661 to 1,095,605
_1082
Position (kb)
1092
1093
1094
1095 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1092.317 kb on + strand, within EX28DRAFT_1079 at 1092.352 kb on + strand, within EX28DRAFT_1079 at 1092.501 kb on + strand, within EX28DRAFT_1080 at 1092.543 kb on + strand, within EX28DRAFT_1080 at 1092.616 kb on - strand, within EX28DRAFT_1080 at 1092.756 kb on + strand, within EX28DRAFT_1080 at 1092.757 kb on - strand, within EX28DRAFT_1080 at 1092.807 kb on + strand, within EX28DRAFT_1080 at 1092.808 kb on - strand, within EX28DRAFT_1080 at 1092.809 kb on + strand, within EX28DRAFT_1080 at 1092.809 kb on + strand, within EX28DRAFT_1080 at 1092.809 kb on + strand, within EX28DRAFT_1080 at 1092.885 kb on + strand at 1092.913 kb on + strand at 1092.914 kb on - strand at 1092.985 kb on + strand at 1092.986 kb on - strand at 1092.986 kb on - strand at 1092.986 kb on - strand at 1093.061 kb on + strand at 1093.065 kb on + strand at 1093.065 kb on + strand at 1093.066 kb on - strand at 1093.066 kb on - strand at 1093.085 kb on + strand, within EX28DRAFT_1081 at 1093.086 kb on - strand, within EX28DRAFT_1081 at 1093.109 kb on + strand, within EX28DRAFT_1081 at 1093.110 kb on - strand, within EX28DRAFT_1081 at 1093.110 kb on - strand, within EX28DRAFT_1081 at 1093.110 kb on - strand, within EX28DRAFT_1081 at 1093.110 kb on - strand, within EX28DRAFT_1081 at 1093.149 kb on + strand, within EX28DRAFT_1081 at 1093.244 kb on + strand, within EX28DRAFT_1081 at 1093.283 kb on + strand, within EX28DRAFT_1081 at 1093.284 kb on - strand, within EX28DRAFT_1081 at 1093.298 kb on + strand, within EX28DRAFT_1081 at 1093.298 kb on + strand, within EX28DRAFT_1081 at 1093.299 kb on - strand, within EX28DRAFT_1081 at 1093.299 kb on - strand, within EX28DRAFT_1081 at 1093.299 kb on - strand, within EX28DRAFT_1081 at 1093.336 kb on + strand, within EX28DRAFT_1081 at 1093.421 kb on + strand, within EX28DRAFT_1081 at 1093.454 kb on + strand, within EX28DRAFT_1081 at 1093.459 kb on - strand, within EX28DRAFT_1081 at 1093.679 kb on + strand, within EX28DRAFT_1081 at 1093.692 kb on - strand, within EX28DRAFT_1081 at 1093.805 kb on + strand, within EX28DRAFT_1081 at 1093.968 kb on - strand at 1094.143 kb on - strand at 1094.175 kb on - strand at 1094.309 kb on + strand at 1094.542 kb on - strand at 1094.580 kb on + strand at 1094.580 kb on + strand at 1094.638 kb on - strand at 1094.662 kb on + strand at 1094.663 kb on - strand at 1094.787 kb on - strand, within EX28DRAFT_1082 at 1094.791 kb on + strand, within EX28DRAFT_1082 at 1095.038 kb on + strand, within EX28DRAFT_1082 at 1095.038 kb on + strand, within EX28DRAFT_1082
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,092,317 + EX28DRAFT_1079 0.86 -0.3 1,092,352 + EX28DRAFT_1079 0.90 +0.4 1,092,501 + EX28DRAFT_1080 0.14 -0.0 1,092,543 + EX28DRAFT_1080 0.23 +1.4 1,092,616 - EX28DRAFT_1080 0.40 +1.1 1,092,756 + EX28DRAFT_1080 0.72 +0.1 1,092,757 - EX28DRAFT_1080 0.72 -1.1 1,092,807 + EX28DRAFT_1080 0.83 -0.4 1,092,808 - EX28DRAFT_1080 0.83 +0.3 1,092,809 + EX28DRAFT_1080 0.84 -0.1 1,092,809 + EX28DRAFT_1080 0.84 -0.6 1,092,809 + EX28DRAFT_1080 0.84 -1.1 1,092,885 + +0.5 1,092,913 + -0.3 1,092,914 - +0.1 1,092,985 + +0.2 1,092,986 - -0.9 1,092,986 - +1.1 1,092,986 - -0.3 1,093,061 + -0.1 1,093,065 + -0.6 1,093,065 + -0.5 1,093,066 - +0.4 1,093,066 - +0.1 1,093,085 + EX28DRAFT_1081 0.12 -1.9 1,093,086 - EX28DRAFT_1081 0.12 -0.9 1,093,109 + EX28DRAFT_1081 0.14 -0.7 1,093,110 - EX28DRAFT_1081 0.14 +0.5 1,093,110 - EX28DRAFT_1081 0.14 +0.6 1,093,110 - EX28DRAFT_1081 0.14 -0.4 1,093,110 - EX28DRAFT_1081 0.14 -0.4 1,093,149 + EX28DRAFT_1081 0.18 +0.0 1,093,244 + EX28DRAFT_1081 0.26 -0.7 1,093,283 + EX28DRAFT_1081 0.30 -0.3 1,093,284 - EX28DRAFT_1081 0.30 -0.8 1,093,298 + EX28DRAFT_1081 0.31 -0.9 1,093,298 + EX28DRAFT_1081 0.31 -0.2 1,093,299 - EX28DRAFT_1081 0.31 +0.3 1,093,299 - EX28DRAFT_1081 0.31 -3.0 1,093,299 - EX28DRAFT_1081 0.31 -0.1 1,093,336 + EX28DRAFT_1081 0.35 +0.2 1,093,421 + EX28DRAFT_1081 0.43 -1.5 1,093,454 + EX28DRAFT_1081 0.46 +1.0 1,093,459 - EX28DRAFT_1081 0.46 +0.1 1,093,679 + EX28DRAFT_1081 0.67 -0.5 1,093,692 - EX28DRAFT_1081 0.68 +0.6 1,093,805 + EX28DRAFT_1081 0.78 +0.3 1,093,968 - +0.3 1,094,143 - -2.1 1,094,175 - -0.6 1,094,309 + +0.2 1,094,542 - -0.5 1,094,580 + -0.8 1,094,580 + +0.0 1,094,638 - -0.3 1,094,662 + +1.2 1,094,663 - +0.3 1,094,787 - EX28DRAFT_1082 0.13 -0.8 1,094,791 + EX28DRAFT_1082 0.14 -0.7 1,095,038 + EX28DRAFT_1082 0.40 -0.4 1,095,038 + EX28DRAFT_1082 0.40 +0.6
Or see this region's nucleotide sequence