Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0940 and EX28DRAFT_0941 are separated by 36 nucleotides EX28DRAFT_0941 and EX28DRAFT_0942 overlap by 4 nucleotides
EX28DRAFT_0940: EX28DRAFT_0940 - pseudouridine synthase, at 939,087 to 939,962
_0940
EX28DRAFT_0941: EX28DRAFT_0941 - cob(I)alamin adenosyltransferase, at 939,999 to 940,589
_0941
EX28DRAFT_0942: EX28DRAFT_0942 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 940,586 to 941,347
_0942
Position (kb)
939
940
941 Strain fitness (log2 ratio)
-1
0
1 at 939.004 kb on + strand at 939.005 kb on - strand at 939.006 kb on + strand at 939.006 kb on + strand at 939.006 kb on + strand at 939.300 kb on + strand, within EX28DRAFT_0940 at 939.304 kb on - strand, within EX28DRAFT_0940 at 939.637 kb on - strand, within EX28DRAFT_0940 at 939.772 kb on - strand, within EX28DRAFT_0940 at 939.801 kb on + strand, within EX28DRAFT_0940 at 940.198 kb on + strand, within EX28DRAFT_0941 at 940.198 kb on + strand, within EX28DRAFT_0941 at 940.199 kb on - strand, within EX28DRAFT_0941 at 940.199 kb on - strand, within EX28DRAFT_0941 at 940.199 kb on - strand, within EX28DRAFT_0941 at 940.316 kb on + strand, within EX28DRAFT_0941 at 940.607 kb on - strand at 940.674 kb on + strand, within EX28DRAFT_0942 at 940.674 kb on + strand, within EX28DRAFT_0942 at 940.684 kb on + strand, within EX28DRAFT_0942 at 940.685 kb on - strand, within EX28DRAFT_0942 at 940.685 kb on - strand, within EX28DRAFT_0942 at 940.854 kb on + strand, within EX28DRAFT_0942 at 941.135 kb on + strand, within EX28DRAFT_0942 at 941.135 kb on + strand, within EX28DRAFT_0942 at 941.149 kb on - strand, within EX28DRAFT_0942 at 941.305 kb on + strand at 941.305 kb on + strand at 941.340 kb on + strand at 941.386 kb on + strand at 941.386 kb on + strand at 941.388 kb on + strand at 941.421 kb on + strand at 941.425 kb on - strand at 941.475 kb on + strand at 941.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 939,004 + +0.1 939,005 - -0.4 939,006 + -0.2 939,006 + -0.4 939,006 + -1.5 939,300 + EX28DRAFT_0940 0.24 -0.1 939,304 - EX28DRAFT_0940 0.25 -1.6 939,637 - EX28DRAFT_0940 0.63 +1.5 939,772 - EX28DRAFT_0940 0.78 -0.1 939,801 + EX28DRAFT_0940 0.82 -0.3 940,198 + EX28DRAFT_0941 0.34 -0.3 940,198 + EX28DRAFT_0941 0.34 +0.2 940,199 - EX28DRAFT_0941 0.34 +0.2 940,199 - EX28DRAFT_0941 0.34 +1.1 940,199 - EX28DRAFT_0941 0.34 +0.5 940,316 + EX28DRAFT_0941 0.54 +0.0 940,607 - +1.4 940,674 + EX28DRAFT_0942 0.12 -0.4 940,674 + EX28DRAFT_0942 0.12 +0.4 940,684 + EX28DRAFT_0942 0.13 +0.1 940,685 - EX28DRAFT_0942 0.13 -0.0 940,685 - EX28DRAFT_0942 0.13 +1.0 940,854 + EX28DRAFT_0942 0.35 +1.4 941,135 + EX28DRAFT_0942 0.72 -0.4 941,135 + EX28DRAFT_0942 0.72 +0.1 941,149 - EX28DRAFT_0942 0.74 +0.2 941,305 + -0.1 941,305 + +0.1 941,340 + -0.5 941,386 + -0.9 941,386 + +0.2 941,388 + -0.1 941,421 + -1.7 941,425 - +1.2 941,475 + +0.1 941,557 - -0.3
Or see this region's nucleotide sequence