Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0901 and EX28DRAFT_0902 overlap by 38 nucleotides EX28DRAFT_0902 and EX28DRAFT_0903 overlap by 8 nucleotides EX28DRAFT_0903 and EX28DRAFT_0904 are separated by 9 nucleotides EX28DRAFT_0904 and EX28DRAFT_0905 are separated by 86 nucleotides EX28DRAFT_0905 and EX28DRAFT_0906 overlap by 4 nucleotides
EX28DRAFT_0901: EX28DRAFT_0901 - hypothetical protein, at 906,591 to 906,749
_0901
EX28DRAFT_0902: EX28DRAFT_0902 - P2 phage tail completion protein R (GpR), at 906,712 to 907,179
_0902
EX28DRAFT_0903: EX28DRAFT_0903 - phage virion morphogenesis (putative tail completion) protein, at 907,172 to 907,624
_0903
EX28DRAFT_0904: EX28DRAFT_0904 - hypothetical protein, at 907,634 to 908,713
_0904
EX28DRAFT_0905: EX28DRAFT_0905 - phage baseplate assembly protein V, at 908,800 to 909,435
_0905
EX28DRAFT_0906: EX28DRAFT_0906 - Phage baseplate assembly protein W, at 909,432 to 909,776
_0906
Position (kb) 907
908
909 Strain fitness (log2 ratio) -2
-1
0
1
2 at 906.637 kb on + strand, within EX28DRAFT_0901 at 906.638 kb on - strand, within EX28DRAFT_0901 at 906.638 kb on - strand, within EX28DRAFT_0901 at 907.085 kb on + strand, within EX28DRAFT_0902 at 907.090 kb on + strand, within EX28DRAFT_0902 at 907.091 kb on - strand, within EX28DRAFT_0902 at 907.091 kb on - strand, within EX28DRAFT_0902 at 907.091 kb on - strand, within EX28DRAFT_0902 at 907.141 kb on + strand at 907.142 kb on - strand at 907.142 kb on - strand at 907.142 kb on - strand at 907.143 kb on + strand at 907.184 kb on - strand at 907.294 kb on + strand, within EX28DRAFT_0903 at 907.295 kb on - strand, within EX28DRAFT_0903 at 907.381 kb on + strand, within EX28DRAFT_0903 at 907.484 kb on - strand, within EX28DRAFT_0903 at 907.525 kb on + strand, within EX28DRAFT_0903 at 907.529 kb on + strand, within EX28DRAFT_0903 at 907.529 kb on + strand, within EX28DRAFT_0903 at 907.530 kb on - strand, within EX28DRAFT_0903 at 907.530 kb on - strand, within EX28DRAFT_0903 at 907.530 kb on - strand, within EX28DRAFT_0903 at 907.533 kb on + strand, within EX28DRAFT_0903 at 907.533 kb on + strand, within EX28DRAFT_0903 at 907.569 kb on + strand, within EX28DRAFT_0903 at 907.569 kb on + strand, within EX28DRAFT_0903 at 907.897 kb on - strand, within EX28DRAFT_0904 at 908.219 kb on - strand, within EX28DRAFT_0904 at 908.307 kb on + strand, within EX28DRAFT_0904 at 908.632 kb on + strand at 908.645 kb on - strand at 908.707 kb on + strand at 908.725 kb on - strand at 908.811 kb on + strand at 908.811 kb on + strand at 908.812 kb on - strand at 909.128 kb on - strand, within EX28DRAFT_0905 at 909.243 kb on + strand, within EX28DRAFT_0905 at 909.244 kb on - strand, within EX28DRAFT_0905 at 909.444 kb on + strand at 909.476 kb on + strand, within EX28DRAFT_0906 at 909.494 kb on + strand, within EX28DRAFT_0906 at 909.713 kb on + strand, within EX28DRAFT_0906 at 909.713 kb on + strand, within EX28DRAFT_0906
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 906,637 + EX28DRAFT_0901 0.29 -2.7 906,638 - EX28DRAFT_0901 0.30 +1.1 906,638 - EX28DRAFT_0901 0.30 -1.0 907,085 + EX28DRAFT_0902 0.80 +1.1 907,090 + EX28DRAFT_0902 0.81 +0.2 907,091 - EX28DRAFT_0902 0.81 +0.5 907,091 - EX28DRAFT_0902 0.81 +0.6 907,091 - EX28DRAFT_0902 0.81 -1.0 907,141 + -0.1 907,142 - -0.1 907,142 - +0.1 907,142 - +0.5 907,143 + +0.7 907,184 - +0.4 907,294 + EX28DRAFT_0903 0.27 +0.1 907,295 - EX28DRAFT_0903 0.27 -0.0 907,381 + EX28DRAFT_0903 0.46 -1.9 907,484 - EX28DRAFT_0903 0.69 +2.2 907,525 + EX28DRAFT_0903 0.78 -1.3 907,529 + EX28DRAFT_0903 0.79 -0.6 907,529 + EX28DRAFT_0903 0.79 -0.3 907,530 - EX28DRAFT_0903 0.79 -0.5 907,530 - EX28DRAFT_0903 0.79 +0.2 907,530 - EX28DRAFT_0903 0.79 +0.4 907,533 + EX28DRAFT_0903 0.80 -0.3 907,533 + EX28DRAFT_0903 0.80 +0.9 907,569 + EX28DRAFT_0903 0.88 +0.3 907,569 + EX28DRAFT_0903 0.88 -2.6 907,897 - EX28DRAFT_0904 0.24 +0.9 908,219 - EX28DRAFT_0904 0.54 +1.1 908,307 + EX28DRAFT_0904 0.62 +0.6 908,632 + -0.1 908,645 - -1.7 908,707 + -0.6 908,725 - +0.7 908,811 + -0.4 908,811 + +0.3 908,812 - +0.6 909,128 - EX28DRAFT_0905 0.52 +0.6 909,243 + EX28DRAFT_0905 0.70 +0.4 909,244 - EX28DRAFT_0905 0.70 +0.6 909,444 + +1.0 909,476 + EX28DRAFT_0906 0.13 +0.9 909,494 + EX28DRAFT_0906 0.18 +0.2 909,713 + EX28DRAFT_0906 0.81 +0.3 909,713 + EX28DRAFT_0906 0.81 +0.0
Or see this region's nucleotide sequence