Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0758 and EX28DRAFT_0759 are separated by 123 nucleotides EX28DRAFT_0759 and EX28DRAFT_0760 are separated by 47 nucleotides EX28DRAFT_0760 and EX28DRAFT_0761 are separated by 93 nucleotides EX28DRAFT_0761 and EX28DRAFT_0762 are separated by 123 nucleotides
EX28DRAFT_0758: EX28DRAFT_0758 - septum site-determining protein MinC, at 755,065 to 755,772
_0758
EX28DRAFT_0759: EX28DRAFT_0759 - Uncharacterized protein conserved in bacteria, at 755,896 to 756,171
_0759
EX28DRAFT_0760: EX28DRAFT_0760 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 756,219 to 756,878
_0760
EX28DRAFT_0761: EX28DRAFT_0761 - Uncharacterized conserved protein, at 756,972 to 757,412
_0761
EX28DRAFT_0762: EX28DRAFT_0762 - Disulfide bond formation protein DsbB, at 757,536 to 758,066
_0762
Position (kb) 756
757 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 755.264 kb on + strand, within EX28DRAFT_0758 at 755.267 kb on - strand, within EX28DRAFT_0758 at 755.319 kb on + strand, within EX28DRAFT_0758 at 755.550 kb on + strand, within EX28DRAFT_0758 at 755.728 kb on + strand at 755.739 kb on + strand at 755.794 kb on - strand at 755.797 kb on - strand at 755.844 kb on + strand at 755.845 kb on - strand at 755.856 kb on + strand at 755.912 kb on - strand at 755.919 kb on + strand at 755.938 kb on + strand, within EX28DRAFT_0759 at 755.944 kb on + strand, within EX28DRAFT_0759 at 755.945 kb on - strand, within EX28DRAFT_0759 at 755.988 kb on - strand, within EX28DRAFT_0759 at 756.042 kb on + strand, within EX28DRAFT_0759 at 756.043 kb on - strand, within EX28DRAFT_0759 at 756.100 kb on + strand, within EX28DRAFT_0759 at 756.100 kb on + strand, within EX28DRAFT_0759 at 756.103 kb on + strand, within EX28DRAFT_0759 at 756.107 kb on + strand, within EX28DRAFT_0759 at 756.189 kb on + strand at 756.223 kb on - strand at 756.233 kb on + strand at 756.234 kb on - strand at 756.234 kb on - strand at 756.295 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.720 kb on - strand, within EX28DRAFT_0760 at 756.741 kb on + strand, within EX28DRAFT_0760 at 756.764 kb on + strand, within EX28DRAFT_0760 at 756.765 kb on - strand, within EX28DRAFT_0760 at 756.833 kb on - strand at 757.129 kb on + strand, within EX28DRAFT_0761 at 757.130 kb on - strand, within EX28DRAFT_0761 at 757.158 kb on + strand, within EX28DRAFT_0761 at 757.158 kb on + strand, within EX28DRAFT_0761 at 757.238 kb on + strand, within EX28DRAFT_0761 at 757.238 kb on + strand, within EX28DRAFT_0761 at 757.248 kb on + strand, within EX28DRAFT_0761 at 757.331 kb on + strand, within EX28DRAFT_0761 at 757.331 kb on + strand, within EX28DRAFT_0761 at 757.537 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 755,264 + EX28DRAFT_0758 0.28 +0.0 755,267 - EX28DRAFT_0758 0.29 -0.8 755,319 + EX28DRAFT_0758 0.36 -0.8 755,550 + EX28DRAFT_0758 0.69 -0.0 755,728 + -2.1 755,739 + -0.3 755,794 - +0.2 755,797 - +1.4 755,844 + -0.1 755,845 - -0.2 755,856 + -0.3 755,912 - -2.4 755,919 + -1.4 755,938 + EX28DRAFT_0759 0.15 -0.4 755,944 + EX28DRAFT_0759 0.17 +0.3 755,945 - EX28DRAFT_0759 0.18 +1.3 755,988 - EX28DRAFT_0759 0.33 +0.9 756,042 + EX28DRAFT_0759 0.53 -0.3 756,043 - EX28DRAFT_0759 0.53 +0.1 756,100 + EX28DRAFT_0759 0.74 +1.1 756,100 + EX28DRAFT_0759 0.74 +0.5 756,103 + EX28DRAFT_0759 0.75 -1.8 756,107 + EX28DRAFT_0759 0.76 -0.6 756,189 + -0.3 756,223 - +0.3 756,233 + -0.4 756,234 - -0.1 756,234 - -0.9 756,295 - EX28DRAFT_0760 0.12 -0.3 756,354 - EX28DRAFT_0760 0.20 +0.5 756,354 - EX28DRAFT_0760 0.20 +0.2 756,354 - EX28DRAFT_0760 0.20 +2.8 756,720 - EX28DRAFT_0760 0.76 -0.2 756,741 + EX28DRAFT_0760 0.79 +0.1 756,764 + EX28DRAFT_0760 0.83 -0.0 756,765 - EX28DRAFT_0760 0.83 +0.8 756,833 - +1.0 757,129 + EX28DRAFT_0761 0.36 +0.1 757,130 - EX28DRAFT_0761 0.36 +1.1 757,158 + EX28DRAFT_0761 0.42 -0.5 757,158 + EX28DRAFT_0761 0.42 -0.1 757,238 + EX28DRAFT_0761 0.60 -0.5 757,238 + EX28DRAFT_0761 0.60 +1.6 757,248 + EX28DRAFT_0761 0.63 +0.4 757,331 + EX28DRAFT_0761 0.81 +2.8 757,331 + EX28DRAFT_0761 0.81 -1.2 757,537 + -0.9
Or see this region's nucleotide sequence