Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0647 and EX28DRAFT_0648 are separated by 202 nucleotides EX28DRAFT_0648 and EX28DRAFT_0649 are separated by 425 nucleotides EX28DRAFT_0649 and EX28DRAFT_0650 are separated by 42 nucleotides
EX28DRAFT_0647: EX28DRAFT_0647 - Ferritin-like protein, at 640,975 to 641,472
_0647
EX28DRAFT_0648: EX28DRAFT_0648 - YecR-like lipoprotein, at 641,675 to 642,013
_0648
EX28DRAFT_0649: EX28DRAFT_0649 - Protein of unknown function (DUF2766), at 642,439 to 642,690
_0649
EX28DRAFT_0650: EX28DRAFT_0650 - anaerobic c4-dicarboxylate membrane transporter family protein, at 642,733 to 644,076
_0650
Position (kb) 642
643 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 641.532 kb on - strand at 641.562 kb on + strand at 641.719 kb on + strand, within EX28DRAFT_0648 at 641.725 kb on + strand, within EX28DRAFT_0648 at 641.725 kb on + strand, within EX28DRAFT_0648 at 641.725 kb on + strand, within EX28DRAFT_0648 at 641.725 kb on + strand, within EX28DRAFT_0648 at 641.728 kb on - strand, within EX28DRAFT_0648 at 641.818 kb on - strand, within EX28DRAFT_0648 at 641.858 kb on + strand, within EX28DRAFT_0648 at 641.858 kb on + strand, within EX28DRAFT_0648 at 641.859 kb on - strand, within EX28DRAFT_0648 at 641.859 kb on - strand, within EX28DRAFT_0648 at 641.859 kb on - strand, within EX28DRAFT_0648 at 641.862 kb on + strand, within EX28DRAFT_0648 at 641.862 kb on + strand, within EX28DRAFT_0648 at 641.863 kb on - strand, within EX28DRAFT_0648 at 641.863 kb on - strand, within EX28DRAFT_0648 at 641.863 kb on - strand, within EX28DRAFT_0648 at 641.874 kb on + strand, within EX28DRAFT_0648 at 641.962 kb on + strand, within EX28DRAFT_0648 at 641.962 kb on + strand, within EX28DRAFT_0648 at 641.966 kb on - strand, within EX28DRAFT_0648 at 641.966 kb on - strand, within EX28DRAFT_0648 at 642.037 kb on + strand at 642.409 kb on + strand at 642.410 kb on - strand at 642.422 kb on - strand at 642.426 kb on - strand at 642.564 kb on + strand, within EX28DRAFT_0649 at 642.564 kb on + strand, within EX28DRAFT_0649 at 642.564 kb on + strand, within EX28DRAFT_0649 at 642.564 kb on + strand, within EX28DRAFT_0649 at 642.564 kb on + strand, within EX28DRAFT_0649 at 642.713 kb on + strand at 642.713 kb on + strand at 642.713 kb on + strand at 642.713 kb on + strand at 642.713 kb on + strand at 642.713 kb on + strand at 642.713 kb on + strand at 642.714 kb on - strand at 642.714 kb on - strand at 642.714 kb on - strand at 642.714 kb on - strand at 642.714 kb on - strand at 642.734 kb on + strand at 642.805 kb on - strand at 642.908 kb on + strand, within EX28DRAFT_0650 at 643.037 kb on + strand, within EX28DRAFT_0650 at 643.037 kb on + strand, within EX28DRAFT_0650 at 643.082 kb on - strand, within EX28DRAFT_0650 at 643.185 kb on + strand, within EX28DRAFT_0650 at 643.204 kb on - strand, within EX28DRAFT_0650 at 643.234 kb on + strand, within EX28DRAFT_0650 at 643.235 kb on - strand, within EX28DRAFT_0650 at 643.235 kb on - strand, within EX28DRAFT_0650 at 643.235 kb on - strand, within EX28DRAFT_0650 at 643.289 kb on - strand, within EX28DRAFT_0650 at 643.402 kb on + strand, within EX28DRAFT_0650 at 643.426 kb on - strand, within EX28DRAFT_0650 at 643.469 kb on - strand, within EX28DRAFT_0650
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 641,532 - +0.6 641,562 + -2.2 641,719 + EX28DRAFT_0648 0.13 -0.0 641,725 + EX28DRAFT_0648 0.15 -0.2 641,725 + EX28DRAFT_0648 0.15 +0.0 641,725 + EX28DRAFT_0648 0.15 +1.3 641,725 + EX28DRAFT_0648 0.15 +0.1 641,728 - EX28DRAFT_0648 0.16 +0.6 641,818 - EX28DRAFT_0648 0.42 -0.5 641,858 + EX28DRAFT_0648 0.54 -0.4 641,858 + EX28DRAFT_0648 0.54 +0.9 641,859 - EX28DRAFT_0648 0.54 -0.3 641,859 - EX28DRAFT_0648 0.54 -1.2 641,859 - EX28DRAFT_0648 0.54 +0.0 641,862 + EX28DRAFT_0648 0.55 -1.5 641,862 + EX28DRAFT_0648 0.55 -0.9 641,863 - EX28DRAFT_0648 0.55 -2.1 641,863 - EX28DRAFT_0648 0.55 +1.3 641,863 - EX28DRAFT_0648 0.55 +0.1 641,874 + EX28DRAFT_0648 0.59 +0.1 641,962 + EX28DRAFT_0648 0.85 -0.4 641,962 + EX28DRAFT_0648 0.85 -3.5 641,966 - EX28DRAFT_0648 0.86 -0.7 641,966 - EX28DRAFT_0648 0.86 -0.5 642,037 + -1.1 642,409 + -0.8 642,410 - +0.2 642,422 - +0.8 642,426 - -0.5 642,564 + EX28DRAFT_0649 0.50 +0.4 642,564 + EX28DRAFT_0649 0.50 +0.1 642,564 + EX28DRAFT_0649 0.50 +1.4 642,564 + EX28DRAFT_0649 0.50 +0.2 642,564 + EX28DRAFT_0649 0.50 -0.1 642,713 + -0.1 642,713 + +0.3 642,713 + -2.3 642,713 + -1.1 642,713 + -0.1 642,713 + +2.3 642,713 + +0.5 642,714 - -0.2 642,714 - +0.8 642,714 - -1.3 642,714 - +2.0 642,714 - -0.3 642,734 + -1.4 642,805 - -0.3 642,908 + EX28DRAFT_0650 0.13 -0.7 643,037 + EX28DRAFT_0650 0.23 +0.8 643,037 + EX28DRAFT_0650 0.23 +0.2 643,082 - EX28DRAFT_0650 0.26 -3.1 643,185 + EX28DRAFT_0650 0.34 -0.6 643,204 - EX28DRAFT_0650 0.35 +0.0 643,234 + EX28DRAFT_0650 0.37 -0.2 643,235 - EX28DRAFT_0650 0.37 -0.1 643,235 - EX28DRAFT_0650 0.37 +0.1 643,235 - EX28DRAFT_0650 0.37 -0.5 643,289 - EX28DRAFT_0650 0.41 -0.2 643,402 + EX28DRAFT_0650 0.50 -1.7 643,426 - EX28DRAFT_0650 0.52 -0.0 643,469 - EX28DRAFT_0650 0.55 -0.2
Or see this region's nucleotide sequence