Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0435 and EX28DRAFT_0436 are separated by 217 nucleotides EX28DRAFT_0436 and EX28DRAFT_0437 overlap by 7 nucleotides EX28DRAFT_0437 and EX28DRAFT_0438 are separated by 46 nucleotides
EX28DRAFT_0435: EX28DRAFT_0435 - Predicted transcriptional regulators, at 415,398 to 416,138
_0435
EX28DRAFT_0436: EX28DRAFT_0436 - Putative regulator of cell autolysis, at 416,356 to 418,041
_0436
EX28DRAFT_0437: EX28DRAFT_0437 - two component transcriptional regulator, LytTR family, at 418,035 to 418,754
_0437
EX28DRAFT_0438: EX28DRAFT_0438 - Uncharacterized protein conserved in bacteria, at 418,801 to 419,271
_0438
Position (kb)
416
417
418
419 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 415.357 kb on + strand at 415.358 kb on - strand at 415.358 kb on - strand at 415.389 kb on + strand at 415.389 kb on + strand at 415.390 kb on - strand at 415.533 kb on - strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.693 kb on + strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 415.694 kb on - strand, within EX28DRAFT_0435 at 416.049 kb on - strand, within EX28DRAFT_0435 at 416.049 kb on - strand, within EX28DRAFT_0435 at 416.121 kb on + strand at 416.121 kb on + strand at 416.122 kb on - strand at 416.128 kb on + strand at 416.170 kb on - strand at 416.175 kb on + strand at 416.175 kb on + strand at 416.175 kb on + strand at 416.306 kb on - strand at 416.322 kb on - strand at 416.322 kb on - strand at 416.355 kb on + strand at 416.355 kb on + strand at 416.355 kb on + strand at 416.359 kb on + strand at 416.359 kb on + strand at 416.359 kb on + strand at 416.469 kb on - strand at 416.482 kb on - strand at 416.508 kb on + strand at 416.734 kb on + strand, within EX28DRAFT_0436 at 416.734 kb on + strand, within EX28DRAFT_0436 at 416.734 kb on + strand, within EX28DRAFT_0436 at 416.734 kb on + strand, within EX28DRAFT_0436 at 416.735 kb on - strand, within EX28DRAFT_0436 at 416.735 kb on - strand, within EX28DRAFT_0436 at 416.735 kb on - strand, within EX28DRAFT_0436 at 416.735 kb on - strand, within EX28DRAFT_0436 at 416.878 kb on - strand, within EX28DRAFT_0436 at 416.898 kb on - strand, within EX28DRAFT_0436 at 416.931 kb on + strand, within EX28DRAFT_0436 at 416.932 kb on - strand, within EX28DRAFT_0436 at 416.932 kb on - strand, within EX28DRAFT_0436 at 416.965 kb on + strand, within EX28DRAFT_0436 at 416.965 kb on + strand, within EX28DRAFT_0436 at 416.965 kb on + strand, within EX28DRAFT_0436 at 416.966 kb on - strand, within EX28DRAFT_0436 at 416.966 kb on - strand, within EX28DRAFT_0436 at 416.966 kb on - strand, within EX28DRAFT_0436 at 416.967 kb on + strand, within EX28DRAFT_0436 at 416.968 kb on - strand, within EX28DRAFT_0436 at 417.036 kb on + strand, within EX28DRAFT_0436 at 417.073 kb on + strand, within EX28DRAFT_0436 at 417.187 kb on - strand, within EX28DRAFT_0436 at 417.471 kb on + strand, within EX28DRAFT_0436 at 417.734 kb on - strand, within EX28DRAFT_0436 at 417.765 kb on + strand, within EX28DRAFT_0436 at 417.766 kb on - strand, within EX28DRAFT_0436 at 417.766 kb on - strand, within EX28DRAFT_0436 at 417.873 kb on + strand at 417.873 kb on + strand at 417.886 kb on + strand at 418.010 kb on + strand at 418.039 kb on + strand at 418.039 kb on + strand at 418.112 kb on + strand, within EX28DRAFT_0437 at 418.113 kb on - strand, within EX28DRAFT_0437 at 418.115 kb on - strand, within EX28DRAFT_0437 at 418.330 kb on + strand, within EX28DRAFT_0437 at 418.331 kb on - strand, within EX28DRAFT_0437 at 418.471 kb on - strand, within EX28DRAFT_0437 at 418.800 kb on + strand at 418.800 kb on + strand at 418.801 kb on - strand at 418.805 kb on + strand at 418.805 kb on + strand at 418.810 kb on - strand at 418.855 kb on - strand, within EX28DRAFT_0438 at 418.996 kb on + strand, within EX28DRAFT_0438 at 418.997 kb on - strand, within EX28DRAFT_0438
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 415,357 + -2.9 415,358 - -0.6 415,358 - +0.1 415,389 + -0.0 415,389 + -0.5 415,390 - -0.6 415,533 - EX28DRAFT_0435 0.18 +0.4 415,693 + EX28DRAFT_0435 0.40 -0.6 415,693 + EX28DRAFT_0435 0.40 +1.4 415,693 + EX28DRAFT_0435 0.40 +0.3 415,693 + EX28DRAFT_0435 0.40 -0.1 415,694 - EX28DRAFT_0435 0.40 +0.1 415,694 - EX28DRAFT_0435 0.40 +0.1 415,694 - EX28DRAFT_0435 0.40 +0.8 416,049 - EX28DRAFT_0435 0.88 +1.4 416,049 - EX28DRAFT_0435 0.88 +2.2 416,121 + +0.8 416,121 + -0.5 416,122 - -0.6 416,128 + -0.3 416,170 - +0.5 416,175 + -0.8 416,175 + +0.8 416,175 + +0.1 416,306 - +0.5 416,322 - -1.1 416,322 - -2.2 416,355 + -0.7 416,355 + +0.6 416,355 + -0.4 416,359 + +2.2 416,359 + +0.4 416,359 + -0.3 416,469 - -2.6 416,482 - -3.4 416,508 + +0.1 416,734 + EX28DRAFT_0436 0.22 +0.4 416,734 + EX28DRAFT_0436 0.22 -0.3 416,734 + EX28DRAFT_0436 0.22 +0.1 416,734 + EX28DRAFT_0436 0.22 +1.7 416,735 - EX28DRAFT_0436 0.22 -1.4 416,735 - EX28DRAFT_0436 0.22 +0.1 416,735 - EX28DRAFT_0436 0.22 +0.4 416,735 - EX28DRAFT_0436 0.22 +0.0 416,878 - EX28DRAFT_0436 0.31 -0.3 416,898 - EX28DRAFT_0436 0.32 +0.1 416,931 + EX28DRAFT_0436 0.34 +0.4 416,932 - EX28DRAFT_0436 0.34 +0.1 416,932 - EX28DRAFT_0436 0.34 -0.7 416,965 + EX28DRAFT_0436 0.36 +0.6 416,965 + EX28DRAFT_0436 0.36 -0.4 416,965 + EX28DRAFT_0436 0.36 +0.4 416,966 - EX28DRAFT_0436 0.36 -1.4 416,966 - EX28DRAFT_0436 0.36 +0.6 416,966 - EX28DRAFT_0436 0.36 +0.4 416,967 + EX28DRAFT_0436 0.36 +0.8 416,968 - EX28DRAFT_0436 0.36 -0.3 417,036 + EX28DRAFT_0436 0.40 +0.2 417,073 + EX28DRAFT_0436 0.43 +0.6 417,187 - EX28DRAFT_0436 0.49 -1.0 417,471 + EX28DRAFT_0436 0.66 +0.3 417,734 - EX28DRAFT_0436 0.82 +0.4 417,765 + EX28DRAFT_0436 0.84 +0.4 417,766 - EX28DRAFT_0436 0.84 +0.6 417,766 - EX28DRAFT_0436 0.84 -1.1 417,873 + +1.1 417,873 + +2.0 417,886 + +0.3 418,010 + +0.7 418,039 + -0.2 418,039 + -0.2 418,112 + EX28DRAFT_0437 0.11 -0.2 418,113 - EX28DRAFT_0437 0.11 +0.2 418,115 - EX28DRAFT_0437 0.11 -1.1 418,330 + EX28DRAFT_0437 0.41 +0.6 418,331 - EX28DRAFT_0437 0.41 +0.4 418,471 - EX28DRAFT_0437 0.61 -0.6 418,800 + +1.1 418,800 + -0.1 418,801 - -0.4 418,805 + +0.3 418,805 + -1.3 418,810 - +0.1 418,855 - EX28DRAFT_0438 0.11 -0.1 418,996 + EX28DRAFT_0438 0.41 -2.4 418,997 - EX28DRAFT_0438 0.42 +0.7
Or see this region's nucleotide sequence