Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0259 and EX28DRAFT_0260 overlap by 37 nucleotides EX28DRAFT_0260 and EX28DRAFT_0261 overlap by 23 nucleotides EX28DRAFT_0261 and EX28DRAFT_0262 overlap by 1 nucleotides EX28DRAFT_0262 and EX28DRAFT_0263 are separated by 14 nucleotides
EX28DRAFT_0259: EX28DRAFT_0259 - type VI secretion protein, VC_A0110 family, at 221,100 to 222,854
_0259
EX28DRAFT_0260: EX28DRAFT_0260 - type VI secretion protein, VC_A0111 family, at 222,818 to 223,903
_0260
EX28DRAFT_0261: EX28DRAFT_0261 - type VI secretion lipoprotein, VC_A0113 family, at 223,881 to 224,426
_0261
EX28DRAFT_0262: EX28DRAFT_0262 - type VI secretion system lysozyme-like protein, at 224,426 to 224,881
_0262
EX28DRAFT_0263: EX28DRAFT_0263 - hypothetical protein, at 224,896 to 225,345
_0263
Position (kb) 222
223
224 Strain fitness (log2 ratio) -2
-1
0
1
2 at 221.872 kb on + strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.006 kb on - strand, within EX28DRAFT_0259 at 222.010 kb on + strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.011 kb on - strand, within EX28DRAFT_0259 at 222.144 kb on + strand, within EX28DRAFT_0259 at 222.144 kb on + strand, within EX28DRAFT_0259 at 222.207 kb on + strand, within EX28DRAFT_0259 at 222.207 kb on + strand, within EX28DRAFT_0259 at 222.208 kb on - strand, within EX28DRAFT_0259 at 222.210 kb on + strand, within EX28DRAFT_0259 at 222.252 kb on - strand, within EX28DRAFT_0259 at 222.368 kb on + strand, within EX28DRAFT_0259 at 222.617 kb on + strand, within EX28DRAFT_0259 at 222.617 kb on + strand, within EX28DRAFT_0259 at 222.646 kb on - strand, within EX28DRAFT_0259 at 222.765 kb on + strand at 222.766 kb on - strand at 222.766 kb on - strand at 222.786 kb on - strand at 222.900 kb on + strand at 222.901 kb on - strand at 222.901 kb on - strand at 223.035 kb on - strand, within EX28DRAFT_0260 at 223.082 kb on - strand, within EX28DRAFT_0260 at 223.091 kb on + strand, within EX28DRAFT_0260 at 223.092 kb on - strand, within EX28DRAFT_0260 at 223.121 kb on + strand, within EX28DRAFT_0260 at 223.225 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.229 kb on + strand, within EX28DRAFT_0260 at 223.230 kb on - strand, within EX28DRAFT_0260 at 223.528 kb on + strand, within EX28DRAFT_0260 at 223.634 kb on + strand, within EX28DRAFT_0260 at 223.635 kb on - strand, within EX28DRAFT_0260 at 223.635 kb on - strand, within EX28DRAFT_0260 at 223.664 kb on + strand, within EX28DRAFT_0260 at 223.692 kb on + strand, within EX28DRAFT_0260 at 223.820 kb on - strand at 223.839 kb on - strand at 223.898 kb on - strand at 223.938 kb on - strand, within EX28DRAFT_0261 at 224.078 kb on + strand, within EX28DRAFT_0261 at 224.152 kb on - strand, within EX28DRAFT_0261 at 224.249 kb on + strand, within EX28DRAFT_0261 at 224.440 kb on + strand at 224.440 kb on + strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.441 kb on - strand at 224.497 kb on + strand, within EX28DRAFT_0262 at 224.545 kb on + strand, within EX28DRAFT_0262 at 224.709 kb on + strand, within EX28DRAFT_0262 at 224.710 kb on - strand, within EX28DRAFT_0262 at 224.754 kb on - strand, within EX28DRAFT_0262 at 224.809 kb on + strand, within EX28DRAFT_0262 at 224.875 kb on + strand at 224.875 kb on + strand at 224.878 kb on - strand at 224.880 kb on - strand at 224.880 kb on - strand at 224.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 221,872 + EX28DRAFT_0259 0.44 -0.7 222,006 - EX28DRAFT_0259 0.52 +0.5 222,006 - EX28DRAFT_0259 0.52 -0.1 222,006 - EX28DRAFT_0259 0.52 -0.5 222,010 + EX28DRAFT_0259 0.52 +0.3 222,011 - EX28DRAFT_0259 0.52 -1.1 222,011 - EX28DRAFT_0259 0.52 -0.5 222,011 - EX28DRAFT_0259 0.52 -1.5 222,144 + EX28DRAFT_0259 0.59 +0.1 222,144 + EX28DRAFT_0259 0.59 +2.4 222,207 + EX28DRAFT_0259 0.63 -0.3 222,207 + EX28DRAFT_0259 0.63 +0.5 222,208 - EX28DRAFT_0259 0.63 -0.2 222,210 + EX28DRAFT_0259 0.63 +1.2 222,252 - EX28DRAFT_0259 0.66 +0.5 222,368 + EX28DRAFT_0259 0.72 +0.3 222,617 + EX28DRAFT_0259 0.86 +0.4 222,617 + EX28DRAFT_0259 0.86 -0.4 222,646 - EX28DRAFT_0259 0.88 -0.5 222,765 + +0.4 222,766 - +2.0 222,766 - -2.3 222,786 - -0.0 222,900 + -0.2 222,901 - -0.3 222,901 - +0.5 223,035 - EX28DRAFT_0260 0.20 +0.9 223,082 - EX28DRAFT_0260 0.24 +0.9 223,091 + EX28DRAFT_0260 0.25 +0.5 223,092 - EX28DRAFT_0260 0.25 +0.6 223,121 + EX28DRAFT_0260 0.28 -0.3 223,225 + EX28DRAFT_0260 0.37 +1.1 223,229 + EX28DRAFT_0260 0.38 -1.1 223,229 + EX28DRAFT_0260 0.38 +0.4 223,229 + EX28DRAFT_0260 0.38 -0.2 223,230 - EX28DRAFT_0260 0.38 -0.2 223,528 + EX28DRAFT_0260 0.65 -0.3 223,634 + EX28DRAFT_0260 0.75 +0.1 223,635 - EX28DRAFT_0260 0.75 +2.7 223,635 - EX28DRAFT_0260 0.75 +0.7 223,664 + EX28DRAFT_0260 0.78 -0.2 223,692 + EX28DRAFT_0260 0.80 -0.4 223,820 - -0.1 223,839 - -0.1 223,898 - -0.7 223,938 - EX28DRAFT_0261 0.10 +0.0 224,078 + EX28DRAFT_0261 0.36 -1.2 224,152 - EX28DRAFT_0261 0.50 +0.5 224,249 + EX28DRAFT_0261 0.67 -0.1 224,440 + +0.9 224,440 + +1.1 224,441 - +0.8 224,441 - +0.9 224,441 - +0.7 224,441 - +0.2 224,497 + EX28DRAFT_0262 0.16 -0.1 224,545 + EX28DRAFT_0262 0.26 -0.2 224,709 + EX28DRAFT_0262 0.62 +0.9 224,710 - EX28DRAFT_0262 0.62 -1.5 224,754 - EX28DRAFT_0262 0.72 -0.3 224,809 + EX28DRAFT_0262 0.84 +0.6 224,875 + +0.7 224,875 + +0.1 224,878 - -0.1 224,880 - -0.0 224,880 - -0.7 224,889 + -0.3
Or see this region's nucleotide sequence