Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0161 and EX28DRAFT_0162 are separated by 97 nucleotides EX28DRAFT_0162 and EX28DRAFT_0163 overlap by 4 nucleotides EX28DRAFT_0163 and EX28DRAFT_0164 are separated by 370 nucleotides
EX28DRAFT_0161: EX28DRAFT_0161 - Cyanate permease, at 107,247 to 108,476
_0161
EX28DRAFT_0162: EX28DRAFT_0162 - Transcriptional regulator, at 108,574 to 109,464
_0162
EX28DRAFT_0163: EX28DRAFT_0163 - Protein of unknown function (DUF2633), at 109,461 to 109,631
_0163
EX28DRAFT_0164: EX28DRAFT_0164 - FOG: EAL domain, at 110,002 to 112,242
_0164
Position (kb)
108
109
110 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.609 kb on + strand, within EX28DRAFT_0161 at 107.783 kb on + strand, within EX28DRAFT_0161 at 108.102 kb on + strand, within EX28DRAFT_0161 at 108.103 kb on - strand, within EX28DRAFT_0161 at 108.173 kb on - strand, within EX28DRAFT_0161 at 108.335 kb on - strand, within EX28DRAFT_0161 at 108.398 kb on - strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.428 kb on + strand at 108.492 kb on + strand at 108.524 kb on - strand at 108.549 kb on - strand at 108.557 kb on + strand at 108.559 kb on + strand at 108.689 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.861 kb on + strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 108.862 kb on - strand, within EX28DRAFT_0162 at 109.084 kb on - strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.293 kb on + strand, within EX28DRAFT_0162 at 109.294 kb on - strand, within EX28DRAFT_0162 at 109.388 kb on + strand at 109.588 kb on - strand, within EX28DRAFT_0163 at 109.689 kb on - strand at 109.731 kb on - strand at 109.731 kb on - strand at 109.785 kb on + strand at 109.800 kb on - strand at 109.837 kb on + strand at 109.889 kb on + strand at 109.906 kb on - strand at 110.001 kb on + strand at 110.001 kb on + strand at 110.001 kb on + strand at 110.058 kb on + strand at 110.076 kb on - strand at 110.100 kb on + strand at 110.101 kb on - strand at 110.137 kb on + strand at 110.137 kb on + strand at 110.138 kb on - strand at 110.140 kb on + strand at 110.140 kb on + strand at 110.141 kb on - strand at 110.141 kb on - strand at 110.144 kb on + strand at 110.208 kb on - strand at 110.220 kb on + strand at 110.227 kb on + strand, within EX28DRAFT_0164 at 110.434 kb on + strand, within EX28DRAFT_0164 at 110.446 kb on + strand, within EX28DRAFT_0164 at 110.446 kb on + strand, within EX28DRAFT_0164 at 110.446 kb on + strand, within EX28DRAFT_0164
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 107,609 + EX28DRAFT_0161 0.29 +1.0 107,609 + EX28DRAFT_0161 0.29 +1.7 107,609 + EX28DRAFT_0161 0.29 -3.0 107,609 + EX28DRAFT_0161 0.29 -2.0 107,783 + EX28DRAFT_0161 0.44 -1.7 108,102 + EX28DRAFT_0161 0.70 -0.8 108,103 - EX28DRAFT_0161 0.70 +0.2 108,173 - EX28DRAFT_0161 0.75 -1.6 108,335 - EX28DRAFT_0161 0.88 +0.6 108,398 - -0.4 108,428 + +0.7 108,428 + -1.4 108,428 + -0.9 108,492 + +0.6 108,524 - +0.9 108,549 - -0.2 108,557 + -1.4 108,559 + +0.9 108,689 + EX28DRAFT_0162 0.13 -0.1 108,861 + EX28DRAFT_0162 0.32 -1.6 108,861 + EX28DRAFT_0162 0.32 -0.7 108,862 - EX28DRAFT_0162 0.32 -1.0 108,862 - EX28DRAFT_0162 0.32 +1.0 109,084 - EX28DRAFT_0162 0.57 -2.0 109,293 + EX28DRAFT_0162 0.81 -1.9 109,293 + EX28DRAFT_0162 0.81 -0.6 109,293 + EX28DRAFT_0162 0.81 -0.5 109,294 - EX28DRAFT_0162 0.81 -1.4 109,388 + -0.5 109,588 - EX28DRAFT_0163 0.74 +1.0 109,689 - -0.6 109,731 - +2.1 109,731 - +0.1 109,785 + -0.4 109,800 - +0.1 109,837 + -0.4 109,889 + +0.2 109,906 - +0.3 110,001 + +0.0 110,001 + +0.6 110,001 + -0.7 110,058 + +0.0 110,076 - -0.7 110,100 + +0.9 110,101 - -0.1 110,137 + -0.2 110,137 + -0.2 110,138 - -0.1 110,140 + -0.3 110,140 + +0.6 110,141 - -0.7 110,141 - -0.6 110,144 + +1.6 110,208 - +0.0 110,220 + +0.2 110,227 + EX28DRAFT_0164 0.10 +0.5 110,434 + EX28DRAFT_0164 0.19 +0.1 110,446 + EX28DRAFT_0164 0.20 +0.5 110,446 + EX28DRAFT_0164 0.20 +0.6 110,446 + EX28DRAFT_0164 0.20 -0.3
Or see this region's nucleotide sequence