Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0134 and EX28DRAFT_0135 are separated by 55 nucleotides EX28DRAFT_0135 and EX28DRAFT_0136 overlap by 4 nucleotides EX28DRAFT_0136 and EX28DRAFT_0137 are separated by 0 nucleotides EX28DRAFT_0137 and EX28DRAFT_0138 are separated by 58 nucleotides
EX28DRAFT_0134: EX28DRAFT_0134 - amino acid/peptide transporter (Peptide:H+ symporter), bacterial, at 78,291 to 79,835
_0134
EX28DRAFT_0135: EX28DRAFT_0135 - replication restart DNA helicase PriA, at 79,891 to 80,112
_0135
EX28DRAFT_0136: EX28DRAFT_0136 - hypothetical protein, at 80,109 to 80,459
_0136
EX28DRAFT_0137: EX28DRAFT_0137 - Thermolysin metallopeptidase, alpha-helical domain/Thermolysin metallopeptidase, catalytic domain, at 80,460 to 81,479
_0137
EX28DRAFT_0138: EX28DRAFT_0138 - Exodeoxyribonuclease VII large subunit (EC 3.1.11.6), at 81,538 to 82,911
_0138
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 79.890 kb on + strand at 79.890 kb on + strand at 79.890 kb on + strand at 79.891 kb on - strand at 79.891 kb on - strand at 79.891 kb on - strand at 79.962 kb on + strand, within EX28DRAFT_0135 at 79.963 kb on - strand, within EX28DRAFT_0135 at 79.963 kb on - strand, within EX28DRAFT_0135 at 79.965 kb on + strand, within EX28DRAFT_0135 at 80.009 kb on + strand, within EX28DRAFT_0135 at 80.009 kb on + strand, within EX28DRAFT_0135 at 80.010 kb on - strand, within EX28DRAFT_0135 at 80.018 kb on + strand, within EX28DRAFT_0135 at 80.018 kb on + strand, within EX28DRAFT_0135 at 80.022 kb on - strand, within EX28DRAFT_0135 at 80.070 kb on + strand, within EX28DRAFT_0135 at 80.070 kb on + strand, within EX28DRAFT_0135 at 80.071 kb on - strand, within EX28DRAFT_0135 at 80.076 kb on + strand, within EX28DRAFT_0135 at 80.110 kb on + strand at 80.393 kb on + strand, within EX28DRAFT_0136 at 80.461 kb on + strand at 80.461 kb on + strand at 80.462 kb on - strand at 80.462 kb on - strand at 80.605 kb on + strand, within EX28DRAFT_0137 at 80.662 kb on + strand, within EX28DRAFT_0137 at 80.890 kb on + strand, within EX28DRAFT_0137 at 80.891 kb on - strand, within EX28DRAFT_0137 at 80.956 kb on + strand, within EX28DRAFT_0137 at 81.040 kb on - strand, within EX28DRAFT_0137 at 81.109 kb on + strand, within EX28DRAFT_0137 at 81.141 kb on - strand, within EX28DRAFT_0137 at 81.141 kb on - strand, within EX28DRAFT_0137 at 81.191 kb on + strand, within EX28DRAFT_0137 at 81.192 kb on - strand, within EX28DRAFT_0137 at 81.196 kb on - strand, within EX28DRAFT_0137 at 81.199 kb on + strand, within EX28DRAFT_0137 at 81.200 kb on - strand, within EX28DRAFT_0137 at 81.200 kb on - strand, within EX28DRAFT_0137 at 81.200 kb on - strand, within EX28DRAFT_0137 at 81.205 kb on + strand, within EX28DRAFT_0137 at 81.206 kb on - strand, within EX28DRAFT_0137 at 81.206 kb on - strand, within EX28DRAFT_0137 at 81.385 kb on - strand at 81.446 kb on + strand at 81.446 kb on + strand at 81.447 kb on - strand at 81.448 kb on + strand at 81.448 kb on + strand at 81.449 kb on - strand at 81.506 kb on + strand at 81.515 kb on + strand at 81.515 kb on + strand at 81.516 kb on - strand at 81.516 kb on - strand at 81.516 kb on - strand at 81.516 kb on - strand at 81.517 kb on + strand at 81.517 kb on + strand at 81.517 kb on + strand at 81.517 kb on + strand at 81.517 kb on + strand at 81.517 kb on + strand at 81.518 kb on - strand at 81.518 kb on - strand at 81.518 kb on - strand at 81.518 kb on - strand at 81.520 kb on + strand at 81.520 kb on + strand at 81.520 kb on + strand at 81.521 kb on - strand at 81.521 kb on - strand at 81.524 kb on + strand at 81.643 kb on + strand at 81.644 kb on - strand at 81.693 kb on + strand, within EX28DRAFT_0138 at 81.694 kb on - strand, within EX28DRAFT_0138 at 81.910 kb on - strand, within EX28DRAFT_0138 at 81.910 kb on - strand, within EX28DRAFT_0138 at 82.052 kb on + strand, within EX28DRAFT_0138 at 82.053 kb on - strand, within EX28DRAFT_0138 at 82.310 kb on - strand, within EX28DRAFT_0138 at 82.312 kb on + strand, within EX28DRAFT_0138 at 82.312 kb on + strand, within EX28DRAFT_0138 at 82.313 kb on - strand, within EX28DRAFT_0138 at 82.357 kb on + strand, within EX28DRAFT_0138 at 82.358 kb on - strand, within EX28DRAFT_0138 at 82.358 kb on - strand, within EX28DRAFT_0138 at 82.358 kb on - strand, within EX28DRAFT_0138
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 79,890 + +0.1 79,890 + +0.2 79,890 + +0.3 79,891 - -1.0 79,891 - +1.1 79,891 - +0.1 79,962 + EX28DRAFT_0135 0.32 -0.2 79,963 - EX28DRAFT_0135 0.32 +0.1 79,963 - EX28DRAFT_0135 0.32 +0.3 79,965 + EX28DRAFT_0135 0.33 +0.6 80,009 + EX28DRAFT_0135 0.53 +0.4 80,009 + EX28DRAFT_0135 0.53 -0.3 80,010 - EX28DRAFT_0135 0.54 +1.3 80,018 + EX28DRAFT_0135 0.57 +1.3 80,018 + EX28DRAFT_0135 0.57 -0.3 80,022 - EX28DRAFT_0135 0.59 -0.1 80,070 + EX28DRAFT_0135 0.81 -0.4 80,070 + EX28DRAFT_0135 0.81 -0.7 80,071 - EX28DRAFT_0135 0.81 +0.1 80,076 + EX28DRAFT_0135 0.83 -0.7 80,110 + +0.7 80,393 + EX28DRAFT_0136 0.81 +0.8 80,461 + -0.5 80,461 + +0.8 80,462 - -0.8 80,462 - +2.1 80,605 + EX28DRAFT_0137 0.14 -0.9 80,662 + EX28DRAFT_0137 0.20 +1.8 80,890 + EX28DRAFT_0137 0.42 +0.2 80,891 - EX28DRAFT_0137 0.42 +0.8 80,956 + EX28DRAFT_0137 0.49 +0.1 81,040 - EX28DRAFT_0137 0.57 +0.7 81,109 + EX28DRAFT_0137 0.64 +1.2 81,141 - EX28DRAFT_0137 0.67 +0.8 81,141 - EX28DRAFT_0137 0.67 -2.3 81,191 + EX28DRAFT_0137 0.72 -0.2 81,192 - EX28DRAFT_0137 0.72 +0.1 81,196 - EX28DRAFT_0137 0.72 +1.0 81,199 + EX28DRAFT_0137 0.72 -0.3 81,200 - EX28DRAFT_0137 0.73 -1.5 81,200 - EX28DRAFT_0137 0.73 +0.6 81,200 - EX28DRAFT_0137 0.73 +0.3 81,205 + EX28DRAFT_0137 0.73 +0.6 81,206 - EX28DRAFT_0137 0.73 +0.8 81,206 - EX28DRAFT_0137 0.73 -0.2 81,385 - -1.6 81,446 + +0.1 81,446 + -1.4 81,447 - -0.6 81,448 + -0.2 81,448 + -0.4 81,449 - -0.0 81,506 + +0.6 81,515 + -0.0 81,515 + +0.3 81,516 - -0.8 81,516 - -0.8 81,516 - +1.0 81,516 - +0.4 81,517 + -1.2 81,517 + -1.8 81,517 + +0.4 81,517 + +0.3 81,517 + +0.3 81,517 + +0.5 81,518 - +0.3 81,518 - -1.4 81,518 - -1.2 81,518 - +0.7 81,520 + -0.1 81,520 + -0.0 81,520 + +0.4 81,521 - -1.1 81,521 - -0.5 81,524 + -0.4 81,643 + -1.2 81,644 - +0.5 81,693 + EX28DRAFT_0138 0.11 -0.7 81,694 - EX28DRAFT_0138 0.11 -0.5 81,910 - EX28DRAFT_0138 0.27 +0.0 81,910 - EX28DRAFT_0138 0.27 +0.2 82,052 + EX28DRAFT_0138 0.37 -0.4 82,053 - EX28DRAFT_0138 0.37 -2.1 82,310 - EX28DRAFT_0138 0.56 -0.9 82,312 + EX28DRAFT_0138 0.56 -0.3 82,312 + EX28DRAFT_0138 0.56 -0.4 82,313 - EX28DRAFT_0138 0.56 -1.0 82,357 + EX28DRAFT_0138 0.60 +0.1 82,358 - EX28DRAFT_0138 0.60 +1.6 82,358 - EX28DRAFT_0138 0.60 -0.7 82,358 - EX28DRAFT_0138 0.60 -1.8
Or see this region's nucleotide sequence