Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0118 and EX28DRAFT_0119 are separated by 17 nucleotides EX28DRAFT_0119 and EX28DRAFT_0120 overlap by 1 nucleotides EX28DRAFT_0120 and EX28DRAFT_0121 are separated by 90 nucleotides
EX28DRAFT_0118: EX28DRAFT_0118 - SseB protein C-terminal domain/SseB protein N-terminal domain, at 55,860 to 56,636
_0118
EX28DRAFT_0119: EX28DRAFT_0119 - transcriptional regulator, RpiR family, at 56,654 to 57,424
_0119
EX28DRAFT_0120: EX28DRAFT_0120 - Rhodanese-related sulfurtransferase, at 57,424 to 58,272
_0120
EX28DRAFT_0121: EX28DRAFT_0121 - Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases, at 58,363 to 59,730
_0121
Position (kb) 57
58
59 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.513 kb on + strand, within EX28DRAFT_0118 at 56.555 kb on + strand, within EX28DRAFT_0118 at 56.858 kb on - strand, within EX28DRAFT_0119 at 56.935 kb on + strand, within EX28DRAFT_0119 at 56.962 kb on + strand, within EX28DRAFT_0119 at 57.120 kb on + strand, within EX28DRAFT_0119 at 57.120 kb on + strand, within EX28DRAFT_0119 at 57.120 kb on + strand, within EX28DRAFT_0119 at 57.121 kb on - strand, within EX28DRAFT_0119 at 57.121 kb on - strand, within EX28DRAFT_0119 at 57.367 kb on - strand at 57.367 kb on - strand at 57.425 kb on + strand at 57.580 kb on + strand, within EX28DRAFT_0120 at 57.581 kb on - strand, within EX28DRAFT_0120 at 57.581 kb on - strand, within EX28DRAFT_0120 at 57.684 kb on + strand, within EX28DRAFT_0120 at 57.684 kb on + strand, within EX28DRAFT_0120 at 57.684 kb on + strand, within EX28DRAFT_0120 at 57.685 kb on - strand, within EX28DRAFT_0120 at 57.685 kb on - strand, within EX28DRAFT_0120 at 57.685 kb on - strand, within EX28DRAFT_0120 at 57.993 kb on - strand, within EX28DRAFT_0120 at 57.993 kb on - strand, within EX28DRAFT_0120 at 57.993 kb on - strand, within EX28DRAFT_0120 at 58.259 kb on + strand at 58.260 kb on - strand at 58.491 kb on + strand at 58.514 kb on + strand, within EX28DRAFT_0121 at 58.526 kb on - strand, within EX28DRAFT_0121 at 58.526 kb on - strand, within EX28DRAFT_0121 at 58.594 kb on + strand, within EX28DRAFT_0121 at 58.594 kb on + strand, within EX28DRAFT_0121 at 58.637 kb on + strand, within EX28DRAFT_0121 at 58.637 kb on + strand, within EX28DRAFT_0121 at 58.638 kb on - strand, within EX28DRAFT_0121 at 58.638 kb on - strand, within EX28DRAFT_0121 at 58.638 kb on - strand, within EX28DRAFT_0121 at 58.638 kb on - strand, within EX28DRAFT_0121 at 58.638 kb on - strand, within EX28DRAFT_0121 at 58.726 kb on - strand, within EX28DRAFT_0121 at 58.755 kb on + strand, within EX28DRAFT_0121 at 58.811 kb on + strand, within EX28DRAFT_0121 at 58.812 kb on - strand, within EX28DRAFT_0121 at 58.812 kb on - strand, within EX28DRAFT_0121 at 58.874 kb on + strand, within EX28DRAFT_0121 at 58.875 kb on - strand, within EX28DRAFT_0121 at 58.875 kb on - strand, within EX28DRAFT_0121 at 58.875 kb on - strand, within EX28DRAFT_0121 at 58.875 kb on - strand, within EX28DRAFT_0121 at 58.875 kb on - strand, within EX28DRAFT_0121 at 58.916 kb on + strand, within EX28DRAFT_0121 at 58.943 kb on + strand, within EX28DRAFT_0121 at 58.944 kb on - strand, within EX28DRAFT_0121 at 59.067 kb on + strand, within EX28DRAFT_0121 at 59.067 kb on + strand, within EX28DRAFT_0121 at 59.067 kb on + strand, within EX28DRAFT_0121
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 56,513 + EX28DRAFT_0118 0.84 +0.1 56,555 + EX28DRAFT_0118 0.89 -0.5 56,858 - EX28DRAFT_0119 0.26 +0.2 56,935 + EX28DRAFT_0119 0.36 -0.5 56,962 + EX28DRAFT_0119 0.40 -0.1 57,120 + EX28DRAFT_0119 0.60 -0.2 57,120 + EX28DRAFT_0119 0.60 +0.1 57,120 + EX28DRAFT_0119 0.60 +1.2 57,121 - EX28DRAFT_0119 0.61 +0.1 57,121 - EX28DRAFT_0119 0.61 +2.3 57,367 - -1.8 57,367 - +0.6 57,425 + -0.8 57,580 + EX28DRAFT_0120 0.18 -0.4 57,581 - EX28DRAFT_0120 0.18 -0.1 57,581 - EX28DRAFT_0120 0.18 -0.5 57,684 + EX28DRAFT_0120 0.31 -0.4 57,684 + EX28DRAFT_0120 0.31 +0.1 57,684 + EX28DRAFT_0120 0.31 -0.4 57,685 - EX28DRAFT_0120 0.31 -0.6 57,685 - EX28DRAFT_0120 0.31 +0.0 57,685 - EX28DRAFT_0120 0.31 -1.0 57,993 - EX28DRAFT_0120 0.67 +0.2 57,993 - EX28DRAFT_0120 0.67 -2.4 57,993 - EX28DRAFT_0120 0.67 -0.4 58,259 + -0.3 58,260 - +0.2 58,491 + -0.5 58,514 + EX28DRAFT_0121 0.11 -0.3 58,526 - EX28DRAFT_0121 0.12 +0.1 58,526 - EX28DRAFT_0121 0.12 -0.4 58,594 + EX28DRAFT_0121 0.17 -1.4 58,594 + EX28DRAFT_0121 0.17 -0.1 58,637 + EX28DRAFT_0121 0.20 +0.9 58,637 + EX28DRAFT_0121 0.20 -0.5 58,638 - EX28DRAFT_0121 0.20 +1.4 58,638 - EX28DRAFT_0121 0.20 -1.1 58,638 - EX28DRAFT_0121 0.20 -1.0 58,638 - EX28DRAFT_0121 0.20 -0.6 58,638 - EX28DRAFT_0121 0.20 +0.1 58,726 - EX28DRAFT_0121 0.27 -0.1 58,755 + EX28DRAFT_0121 0.29 +0.2 58,811 + EX28DRAFT_0121 0.33 -1.0 58,812 - EX28DRAFT_0121 0.33 +0.4 58,812 - EX28DRAFT_0121 0.33 -0.6 58,874 + EX28DRAFT_0121 0.37 -1.4 58,875 - EX28DRAFT_0121 0.37 -1.7 58,875 - EX28DRAFT_0121 0.37 -0.1 58,875 - EX28DRAFT_0121 0.37 +0.2 58,875 - EX28DRAFT_0121 0.37 +0.6 58,875 - EX28DRAFT_0121 0.37 -0.1 58,916 + EX28DRAFT_0121 0.40 +0.1 58,943 + EX28DRAFT_0121 0.42 +1.3 58,944 - EX28DRAFT_0121 0.42 -0.8 59,067 + EX28DRAFT_0121 0.51 -0.4 59,067 + EX28DRAFT_0121 0.51 +0.2 59,067 + EX28DRAFT_0121 0.51 +0.4
Or see this region's nucleotide sequence