Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02702 and CCNA_02703 overlap by 1 nucleotides CCNA_02703 and CCNA_02704 are separated by 335 nucleotides CCNA_02704 and CCNA_02705 are separated by 120 nucleotides CCNA_02705 and CCNA_R0064 are separated by 246 nucleotides CCNA_R0064 and CCNA_R0065 are separated by 63 nucleotides CCNA_R0065 and CCNA_R0066 are separated by 98 nucleotides
CCNA_02702: CCNA_02702 - xanthine dehydrogenase small subunit, at 2,857,314 to 2,858,762
_02702
CCNA_02703: CCNA_02703 - 5-hydroxyisourate hydrolase, at 2,858,762 to 2,859,109
_02703
CCNA_02704: CCNA_02704 - NADP-dependent malic enzyme, at 2,859,445 to 2,861,721
_02704
CCNA_02705: CCNA_02705 - cold shock protein CspC, at 2,861,842 to 2,862,051
_02705
CCNA_R0064: CCNA_R0064 - tRNA-Met, at 2,862,298 to 2,862,374
_R0064
CCNA_R0065: CCNA_R0065 - 5S ribosomal RNA, at 2,862,438 to 2,862,555
_R0065
CCNA_R0066: CCNA_R0066 - 23S ribosomal RNA, at 2,862,654 to 2,865,446
_R0066
Position (kb)
2859
2860
2861
2862 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2858.446 kb on + strand, within CCNA_02702 at 2858.564 kb on - strand, within CCNA_02702 at 2858.581 kb on - strand, within CCNA_02702 at 2858.583 kb on + strand, within CCNA_02702 at 2858.601 kb on + strand, within CCNA_02702 at 2858.601 kb on + strand, within CCNA_02702 at 2858.609 kb on - strand, within CCNA_02702 at 2858.609 kb on - strand, within CCNA_02702 at 2858.609 kb on - strand, within CCNA_02702 at 2858.611 kb on + strand, within CCNA_02702 at 2858.719 kb on + strand at 2858.719 kb on + strand at 2858.726 kb on - strand at 2858.738 kb on + strand at 2858.758 kb on + strand at 2858.793 kb on + strand at 2858.794 kb on - strand at 2858.814 kb on + strand, within CCNA_02703 at 2858.814 kb on + strand, within CCNA_02703 at 2858.814 kb on + strand, within CCNA_02703 at 2858.906 kb on - strand, within CCNA_02703 at 2858.906 kb on - strand, within CCNA_02703 at 2859.139 kb on + strand at 2859.158 kb on + strand at 2859.166 kb on - strand at 2859.171 kb on - strand at 2859.171 kb on - strand at 2859.174 kb on + strand at 2859.188 kb on + strand at 2859.214 kb on - strand at 2859.342 kb on + strand at 2859.342 kb on + strand at 2859.350 kb on - strand at 2859.350 kb on - strand at 2859.357 kb on + strand at 2859.365 kb on - strand at 2859.401 kb on + strand at 2859.610 kb on - strand at 2859.618 kb on + strand at 2859.797 kb on + strand, within CCNA_02704 at 2859.855 kb on + strand, within CCNA_02704 at 2860.004 kb on - strand, within CCNA_02704 at 2860.004 kb on - strand, within CCNA_02704 at 2860.004 kb on - strand, within CCNA_02704 at 2860.307 kb on - strand, within CCNA_02704 at 2860.390 kb on - strand, within CCNA_02704 at 2860.525 kb on + strand, within CCNA_02704 at 2860.697 kb on + strand, within CCNA_02704 at 2860.697 kb on + strand, within CCNA_02704 at 2860.816 kb on + strand, within CCNA_02704 at 2860.816 kb on + strand, within CCNA_02704 at 2861.131 kb on + strand, within CCNA_02704 at 2861.389 kb on + strand, within CCNA_02704 at 2861.610 kb on + strand at 2862.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,858,446 + CCNA_02702 0.78 -0.1 2,858,564 - CCNA_02702 0.86 +0.1 2,858,581 - CCNA_02702 0.87 -0.3 2,858,583 + CCNA_02702 0.88 -0.0 2,858,601 + CCNA_02702 0.89 -0.4 2,858,601 + CCNA_02702 0.89 -0.2 2,858,609 - CCNA_02702 0.89 +0.3 2,858,609 - CCNA_02702 0.89 +1.1 2,858,609 - CCNA_02702 0.89 +1.2 2,858,611 + CCNA_02702 0.90 -1.0 2,858,719 + -0.7 2,858,719 + -0.2 2,858,726 - +1.1 2,858,738 + -0.6 2,858,758 + +1.5 2,858,793 + -1.4 2,858,794 - -1.0 2,858,814 + CCNA_02703 0.15 +0.3 2,858,814 + CCNA_02703 0.15 -2.1 2,858,814 + CCNA_02703 0.15 -0.4 2,858,906 - CCNA_02703 0.41 -0.6 2,858,906 - CCNA_02703 0.41 +1.8 2,859,139 + +0.9 2,859,158 + +0.2 2,859,166 - +0.5 2,859,171 - -0.9 2,859,171 - -0.0 2,859,174 + -0.8 2,859,188 + -0.7 2,859,214 - -0.2 2,859,342 + -1.0 2,859,342 + +1.8 2,859,350 - -0.7 2,859,350 - +0.6 2,859,357 + +0.9 2,859,365 - -0.1 2,859,401 + +0.2 2,859,610 - +0.8 2,859,618 + -0.6 2,859,797 + CCNA_02704 0.15 -0.0 2,859,855 + CCNA_02704 0.18 +0.8 2,860,004 - CCNA_02704 0.25 -0.7 2,860,004 - CCNA_02704 0.25 +0.6 2,860,004 - CCNA_02704 0.25 +1.1 2,860,307 - CCNA_02704 0.38 +0.7 2,860,390 - CCNA_02704 0.42 -0.5 2,860,525 + CCNA_02704 0.47 -0.9 2,860,697 + CCNA_02704 0.55 +1.2 2,860,697 + CCNA_02704 0.55 +0.6 2,860,816 + CCNA_02704 0.60 -0.5 2,860,816 + CCNA_02704 0.60 -0.5 2,861,131 + CCNA_02704 0.74 -0.0 2,861,389 + CCNA_02704 0.85 -0.9 2,861,610 + +0.8 2,862,132 - -0.5
Or see this region's nucleotide sequence