Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02333 and CCNA_02334 are separated by 117 nucleotides CCNA_02334 and CCNA_02335 are separated by 62 nucleotides CCNA_02335 and CCNA_02336 overlap by 26 nucleotides
CCNA_02333: CCNA_02333 - lipid A biosynthesis lauroyl acyltransferase, at 2,474,478 to 2,475,422
_02333
CCNA_02334: CCNA_02334 - transcriptional regulator of stalk biogenesis staR, at 2,475,540 to 2,475,935
_02334
CCNA_02335: CCNA_02335 - inositol monophosphatase family protein, at 2,475,998 to 2,476,834
_02335
CCNA_02336: CCNA_02336 - lysophospholipase L2, at 2,476,809 to 2,477,789
_02336
Position (kb)
2475
2476 Strain fitness (log2 ratio)
-1
0
1
2 at 2474.809 kb on + strand, within CCNA_02333 at 2474.850 kb on - strand, within CCNA_02333 at 2474.852 kb on + strand, within CCNA_02333 at 2474.860 kb on - strand, within CCNA_02333 at 2474.932 kb on + strand, within CCNA_02333 at 2475.370 kb on + strand at 2475.418 kb on + strand at 2475.481 kb on - strand at 2475.488 kb on - strand at 2475.527 kb on - strand at 2475.565 kb on + strand at 2475.567 kb on - strand at 2475.610 kb on + strand, within CCNA_02334 at 2475.618 kb on - strand, within CCNA_02334 at 2475.704 kb on + strand, within CCNA_02334 at 2475.716 kb on + strand, within CCNA_02334 at 2475.723 kb on + strand, within CCNA_02334 at 2475.738 kb on - strand, within CCNA_02334 at 2475.765 kb on - strand, within CCNA_02334 at 2475.769 kb on - strand, within CCNA_02334 at 2475.769 kb on - strand, within CCNA_02334 at 2475.769 kb on - strand, within CCNA_02334 at 2475.829 kb on + strand, within CCNA_02334 at 2475.829 kb on + strand, within CCNA_02334 at 2475.837 kb on - strand, within CCNA_02334 at 2475.847 kb on - strand, within CCNA_02334 at 2475.887 kb on + strand, within CCNA_02334 at 2475.938 kb on - strand at 2475.955 kb on - strand at 2475.980 kb on - strand at 2475.982 kb on + strand at 2475.982 kb on + strand at 2475.982 kb on + strand at 2475.990 kb on - strand at 2475.990 kb on - strand at 2476.084 kb on + strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.092 kb on - strand, within CCNA_02335 at 2476.095 kb on + strand, within CCNA_02335 at 2476.101 kb on + strand, within CCNA_02335 at 2476.121 kb on + strand, within CCNA_02335 at 2476.165 kb on - strand, within CCNA_02335 at 2476.179 kb on - strand, within CCNA_02335 at 2476.237 kb on + strand, within CCNA_02335 at 2476.341 kb on - strand, within CCNA_02335 at 2476.351 kb on - strand, within CCNA_02335 at 2476.376 kb on + strand, within CCNA_02335 at 2476.471 kb on - strand, within CCNA_02335 at 2476.471 kb on - strand, within CCNA_02335 at 2476.542 kb on + strand, within CCNA_02335 at 2476.550 kb on - strand, within CCNA_02335 at 2476.648 kb on + strand, within CCNA_02335 at 2476.749 kb on + strand, within CCNA_02335 at 2476.771 kb on + strand at 2476.779 kb on - strand at 2476.820 kb on - strand at 2476.887 kb on - strand at 2476.887 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,474,809 + CCNA_02333 0.35 -0.3 2,474,850 - CCNA_02333 0.39 -0.0 2,474,852 + CCNA_02333 0.40 +2.0 2,474,860 - CCNA_02333 0.40 +0.4 2,474,932 + CCNA_02333 0.48 +0.1 2,475,370 + -1.7 2,475,418 + -0.8 2,475,481 - +0.0 2,475,488 - +0.3 2,475,527 - -0.0 2,475,565 + +0.3 2,475,567 - +0.5 2,475,610 + CCNA_02334 0.18 +0.2 2,475,618 - CCNA_02334 0.20 +0.1 2,475,704 + CCNA_02334 0.41 +0.0 2,475,716 + CCNA_02334 0.44 +0.9 2,475,723 + CCNA_02334 0.46 +0.5 2,475,738 - CCNA_02334 0.50 +0.0 2,475,765 - CCNA_02334 0.57 +0.9 2,475,769 - CCNA_02334 0.58 -0.8 2,475,769 - CCNA_02334 0.58 -0.5 2,475,769 - CCNA_02334 0.58 -1.3 2,475,829 + CCNA_02334 0.73 +0.1 2,475,829 + CCNA_02334 0.73 -0.2 2,475,837 - CCNA_02334 0.75 -1.2 2,475,847 - CCNA_02334 0.78 +1.3 2,475,887 + CCNA_02334 0.88 -0.2 2,475,938 - -1.4 2,475,955 - -0.4 2,475,980 - +0.3 2,475,982 + +0.6 2,475,982 + +0.1 2,475,982 + -0.2 2,475,990 - -0.7 2,475,990 - +0.0 2,476,084 + CCNA_02335 0.10 +0.3 2,476,092 - CCNA_02335 0.11 +0.2 2,476,092 - CCNA_02335 0.11 +0.9 2,476,092 - CCNA_02335 0.11 +0.2 2,476,092 - CCNA_02335 0.11 -0.5 2,476,092 - CCNA_02335 0.11 -0.1 2,476,092 - CCNA_02335 0.11 +1.7 2,476,092 - CCNA_02335 0.11 +0.2 2,476,095 + CCNA_02335 0.12 -0.3 2,476,101 + CCNA_02335 0.12 -0.2 2,476,121 + CCNA_02335 0.15 -1.7 2,476,165 - CCNA_02335 0.20 -0.0 2,476,179 - CCNA_02335 0.22 +0.2 2,476,237 + CCNA_02335 0.29 -0.8 2,476,341 - CCNA_02335 0.41 +0.0 2,476,351 - CCNA_02335 0.42 +1.5 2,476,376 + CCNA_02335 0.45 +0.2 2,476,471 - CCNA_02335 0.57 +1.1 2,476,471 - CCNA_02335 0.57 +0.2 2,476,542 + CCNA_02335 0.65 -0.4 2,476,550 - CCNA_02335 0.66 +0.4 2,476,648 + CCNA_02335 0.78 +0.6 2,476,749 + CCNA_02335 0.90 +0.3 2,476,771 + +0.3 2,476,779 - -1.0 2,476,820 - +0.7 2,476,887 - +0.1 2,476,887 - -0.9
Or see this region's nucleotide sequence