Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02284 and CCNA_02285 are separated by 51 nucleotides CCNA_02285 and CCNA_02286 are separated by 177 nucleotides CCNA_02286 and CCNA_02287 are separated by 167 nucleotides CCNA_02287 and CCNA_02288 overlap by 4 nucleotides
CCNA_02284: CCNA_02284 - DNA-3-methyladenine glycosylase II, at 2,434,123 to 2,434,764
_02284
CCNA_02285: CCNA_02285 - transcription elongation factor GreB, at 2,434,816 to 2,435,298
_02285
CCNA_02286: CCNA_02286 - hypothetical protein, at 2,435,476 to 2,435,586
_02286
CCNA_02287: CCNA_02287 - TetR-family transcriptional regulator, at 2,435,754 to 2,436,383
_02287
CCNA_02288: CCNA_02288 - tRNA synthetase class I, catalytic domain protein, at 2,436,380 to 2,437,219
_02288
Position (kb)
2435
2436 Strain fitness (log2 ratio)
-2
-1
0
1 at 2434.477 kb on - strand, within CCNA_02284 at 2434.506 kb on + strand, within CCNA_02284 at 2434.540 kb on + strand, within CCNA_02284 at 2434.579 kb on - strand, within CCNA_02284 at 2434.603 kb on - strand, within CCNA_02284 at 2434.650 kb on - strand, within CCNA_02284 at 2434.876 kb on - strand, within CCNA_02285 at 2434.887 kb on + strand, within CCNA_02285 at 2434.915 kb on - strand, within CCNA_02285 at 2434.936 kb on + strand, within CCNA_02285 at 2434.936 kb on + strand, within CCNA_02285 at 2434.936 kb on + strand, within CCNA_02285 at 2434.936 kb on + strand, within CCNA_02285 at 2434.936 kb on + strand, within CCNA_02285 at 2434.942 kb on + strand, within CCNA_02285 at 2434.964 kb on + strand, within CCNA_02285 at 2434.964 kb on + strand, within CCNA_02285 at 2434.964 kb on + strand, within CCNA_02285 at 2434.972 kb on - strand, within CCNA_02285 at 2435.001 kb on - strand, within CCNA_02285 at 2435.019 kb on - strand, within CCNA_02285 at 2435.069 kb on + strand, within CCNA_02285 at 2435.082 kb on + strand, within CCNA_02285 at 2435.098 kb on + strand, within CCNA_02285 at 2435.098 kb on + strand, within CCNA_02285 at 2435.098 kb on + strand, within CCNA_02285 at 2435.149 kb on + strand, within CCNA_02285 at 2435.204 kb on + strand, within CCNA_02285 at 2435.223 kb on - strand, within CCNA_02285 at 2435.317 kb on - strand at 2435.370 kb on - strand at 2435.377 kb on + strand at 2435.382 kb on + strand at 2435.440 kb on + strand at 2435.448 kb on - strand at 2435.574 kb on + strand, within CCNA_02286 at 2435.611 kb on + strand at 2435.621 kb on + strand at 2435.743 kb on + strand at 2435.746 kb on - strand at 2435.746 kb on - strand at 2435.790 kb on - strand at 2435.796 kb on + strand at 2435.851 kb on + strand, within CCNA_02287 at 2435.859 kb on + strand, within CCNA_02287 at 2435.924 kb on + strand, within CCNA_02287 at 2435.939 kb on + strand, within CCNA_02287 at 2435.939 kb on + strand, within CCNA_02287 at 2436.045 kb on + strand, within CCNA_02287 at 2436.045 kb on + strand, within CCNA_02287 at 2436.053 kb on - strand, within CCNA_02287 at 2436.053 kb on - strand, within CCNA_02287 at 2436.090 kb on + strand, within CCNA_02287 at 2436.090 kb on + strand, within CCNA_02287 at 2436.090 kb on + strand, within CCNA_02287 at 2436.098 kb on - strand, within CCNA_02287 at 2436.131 kb on + strand, within CCNA_02287 at 2436.131 kb on + strand, within CCNA_02287 at 2436.268 kb on + strand, within CCNA_02287 at 2436.268 kb on + strand, within CCNA_02287 at 2436.276 kb on - strand, within CCNA_02287 at 2436.287 kb on + strand, within CCNA_02287 at 2436.295 kb on - strand, within CCNA_02287 at 2436.295 kb on - strand, within CCNA_02287 at 2436.323 kb on - strand at 2436.340 kb on - strand at 2436.344 kb on + strand at 2436.397 kb on + strand at 2436.417 kb on - strand at 2436.516 kb on - strand, within CCNA_02288 at 2436.521 kb on - strand, within CCNA_02288 at 2436.557 kb on + strand, within CCNA_02288 at 2436.572 kb on + strand, within CCNA_02288 at 2436.585 kb on - strand, within CCNA_02288
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,434,477 - CCNA_02284 0.55 +0.4 2,434,506 + CCNA_02284 0.60 -0.4 2,434,540 + CCNA_02284 0.65 -0.3 2,434,579 - CCNA_02284 0.71 +1.3 2,434,603 - CCNA_02284 0.75 -0.1 2,434,650 - CCNA_02284 0.82 +0.3 2,434,876 - CCNA_02285 0.12 +0.2 2,434,887 + CCNA_02285 0.15 +0.3 2,434,915 - CCNA_02285 0.20 +1.1 2,434,936 + CCNA_02285 0.25 -1.1 2,434,936 + CCNA_02285 0.25 +0.0 2,434,936 + CCNA_02285 0.25 +0.1 2,434,936 + CCNA_02285 0.25 +0.7 2,434,936 + CCNA_02285 0.25 -0.2 2,434,942 + CCNA_02285 0.26 +0.7 2,434,964 + CCNA_02285 0.31 +0.2 2,434,964 + CCNA_02285 0.31 -1.7 2,434,964 + CCNA_02285 0.31 -0.8 2,434,972 - CCNA_02285 0.32 +0.3 2,435,001 - CCNA_02285 0.38 +0.1 2,435,019 - CCNA_02285 0.42 +0.2 2,435,069 + CCNA_02285 0.52 +0.2 2,435,082 + CCNA_02285 0.55 +0.2 2,435,098 + CCNA_02285 0.58 +0.3 2,435,098 + CCNA_02285 0.58 -0.3 2,435,098 + CCNA_02285 0.58 +0.3 2,435,149 + CCNA_02285 0.69 -0.0 2,435,204 + CCNA_02285 0.80 -0.5 2,435,223 - CCNA_02285 0.84 -0.1 2,435,317 - +0.2 2,435,370 - -0.4 2,435,377 + -0.0 2,435,382 + -0.3 2,435,440 + -0.2 2,435,448 - -0.4 2,435,574 + CCNA_02286 0.88 -2.7 2,435,611 + +0.0 2,435,621 + +0.1 2,435,743 + +1.0 2,435,746 - +0.7 2,435,746 - +1.1 2,435,790 - -0.3 2,435,796 + -0.2 2,435,851 + CCNA_02287 0.15 +1.1 2,435,859 + CCNA_02287 0.17 +0.3 2,435,924 + CCNA_02287 0.27 +0.2 2,435,939 + CCNA_02287 0.29 -0.2 2,435,939 + CCNA_02287 0.29 -0.9 2,436,045 + CCNA_02287 0.46 +0.8 2,436,045 + CCNA_02287 0.46 +0.6 2,436,053 - CCNA_02287 0.47 -0.9 2,436,053 - CCNA_02287 0.47 -2.3 2,436,090 + CCNA_02287 0.53 +0.5 2,436,090 + CCNA_02287 0.53 +0.2 2,436,090 + CCNA_02287 0.53 -0.0 2,436,098 - CCNA_02287 0.55 -1.4 2,436,131 + CCNA_02287 0.60 +0.1 2,436,131 + CCNA_02287 0.60 +0.6 2,436,268 + CCNA_02287 0.82 +0.9 2,436,268 + CCNA_02287 0.82 +0.1 2,436,276 - CCNA_02287 0.83 -1.5 2,436,287 + CCNA_02287 0.85 +0.5 2,436,295 - CCNA_02287 0.86 +0.7 2,436,295 - CCNA_02287 0.86 -1.5 2,436,323 - -1.7 2,436,340 - -0.9 2,436,344 + +0.7 2,436,397 + +0.4 2,436,417 - -1.1 2,436,516 - CCNA_02288 0.16 -0.3 2,436,521 - CCNA_02288 0.17 -0.1 2,436,557 + CCNA_02288 0.21 +0.9 2,436,572 + CCNA_02288 0.23 -1.1 2,436,585 - CCNA_02288 0.24 -0.3
Or see this region's nucleotide sequence