Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02277 and CCNA_02278 are separated by 106 nucleotides CCNA_02278 and CCNA_02279 overlap by 4 nucleotides CCNA_02279 and CCNA_02280 overlap by 4 nucleotides CCNA_02280 and CCNA_02281 are separated by 91 nucleotides
CCNA_02277: CCNA_02277 - TonB-dependent outer membrane channel, at 2,426,768 to 2,428,969
_02277
CCNA_02278: CCNA_02278 - hypothetical protein, at 2,429,076 to 2,429,684
_02278
CCNA_02279: CCNA_02279 - disulfide bond formation protein B, at 2,429,681 to 2,430,310
_02279
CCNA_02280: CCNA_02280 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 2,430,307 to 2,430,867
_02280
CCNA_02281: CCNA_02281 - membrane alanine aminopeptidase, at 2,430,959 to 2,432,740
_02281
Position (kb)
2429
2430
2431 Strain fitness (log2 ratio)
-1
0
1 at 2428.736 kb on + strand, within CCNA_02277 at 2428.839 kb on + strand at 2428.891 kb on + strand at 2429.623 kb on - strand, within CCNA_02278 at 2429.627 kb on - strand at 2429.695 kb on + strand at 2429.748 kb on - strand, within CCNA_02279 at 2429.748 kb on - strand, within CCNA_02279 at 2429.962 kb on + strand, within CCNA_02279 at 2430.301 kb on - strand at 2430.304 kb on + strand at 2431.063 kb on - strand at 2431.082 kb on - strand at 2431.084 kb on - strand at 2431.184 kb on + strand, within CCNA_02281 at 2431.185 kb on + strand, within CCNA_02281 at 2431.187 kb on - strand, within CCNA_02281 at 2431.211 kb on - strand, within CCNA_02281 at 2431.221 kb on - strand, within CCNA_02281 at 2431.302 kb on + strand, within CCNA_02281
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,428,736 + CCNA_02277 0.89 -0.1 2,428,839 + +0.0 2,428,891 + +0.1 2,429,623 - CCNA_02278 0.90 -0.8 2,429,627 - -0.8 2,429,695 + +0.5 2,429,748 - CCNA_02279 0.11 -1.3 2,429,748 - CCNA_02279 0.11 -0.6 2,429,962 + CCNA_02279 0.45 -0.1 2,430,301 - -1.2 2,430,304 + +0.2 2,431,063 - -0.3 2,431,082 - +0.8 2,431,084 - -1.0 2,431,184 + CCNA_02281 0.13 +0.6 2,431,185 + CCNA_02281 0.13 -0.6 2,431,187 - CCNA_02281 0.13 -0.1 2,431,211 - CCNA_02281 0.14 -0.1 2,431,221 - CCNA_02281 0.15 +0.1 2,431,302 + CCNA_02281 0.19 +0.0
Or see this region's nucleotide sequence