Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02247 and CCNA_02248 are separated by 1 nucleotides CCNA_02248 and CCNA_02249 are separated by 74 nucleotides CCNA_02249 and CCNA_02250 are separated by 207 nucleotides
CCNA_02247: CCNA_02247 - pantoate-beta-alanine ligase, at 2,396,214 to 2,397,071
_02247
CCNA_02248: CCNA_02248 - hypothetical protein, at 2,397,073 to 2,397,162
_02248
CCNA_02249: CCNA_02249 - conserved hypothetical cytosolic protein, at 2,397,237 to 2,397,635
_02249
CCNA_02250: CCNA_02250 - methylcrotonyl-CoA carboxylase biotin-containing subunit, at 2,397,843 to 2,399,807
_02250
Position (kb)
2397
2398 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2396.107 kb on + strand at 2396.122 kb on - strand at 2396.162 kb on - strand at 2396.177 kb on + strand at 2396.185 kb on - strand at 2396.185 kb on - strand at 2397.108 kb on + strand, within CCNA_02248 at 2397.113 kb on + strand, within CCNA_02248 at 2397.171 kb on + strand at 2397.171 kb on + strand at 2397.179 kb on - strand at 2397.199 kb on - strand at 2397.364 kb on - strand, within CCNA_02249 at 2397.470 kb on - strand, within CCNA_02249 at 2397.470 kb on - strand, within CCNA_02249 at 2397.475 kb on - strand, within CCNA_02249 at 2397.484 kb on + strand, within CCNA_02249 at 2397.484 kb on + strand, within CCNA_02249 at 2397.557 kb on - strand, within CCNA_02249 at 2397.577 kb on + strand, within CCNA_02249 at 2397.614 kb on + strand at 2397.624 kb on + strand at 2397.627 kb on - strand at 2397.637 kb on - strand at 2397.639 kb on - strand at 2397.646 kb on - strand at 2397.659 kb on + strand at 2397.667 kb on - strand at 2397.771 kb on - strand at 2397.771 kb on - strand at 2397.771 kb on - strand at 2397.846 kb on + strand at 2397.846 kb on + strand at 2397.849 kb on - strand at 2397.865 kb on - strand at 2397.867 kb on + strand at 2397.867 kb on + strand at 2397.875 kb on - strand at 2397.943 kb on + strand at 2397.968 kb on + strand at 2397.984 kb on + strand at 2398.025 kb on - strand at 2398.124 kb on - strand, within CCNA_02250
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,396,107 + +2.6 2,396,122 - +0.8 2,396,162 - -0.3 2,396,177 + +0.2 2,396,185 - +0.3 2,396,185 - -0.0 2,397,108 + CCNA_02248 0.39 -1.2 2,397,113 + CCNA_02248 0.44 -0.8 2,397,171 + +0.2 2,397,171 + -0.1 2,397,179 - +1.5 2,397,199 - +0.6 2,397,364 - CCNA_02249 0.32 +0.5 2,397,470 - CCNA_02249 0.58 +1.0 2,397,470 - CCNA_02249 0.58 +0.4 2,397,475 - CCNA_02249 0.60 -0.4 2,397,484 + CCNA_02249 0.62 -0.4 2,397,484 + CCNA_02249 0.62 +0.1 2,397,557 - CCNA_02249 0.80 -0.1 2,397,577 + CCNA_02249 0.85 -0.1 2,397,614 + -0.1 2,397,624 + +0.2 2,397,627 - +0.3 2,397,637 - +0.2 2,397,639 - +0.8 2,397,646 - +1.0 2,397,659 + +0.2 2,397,667 - +0.2 2,397,771 - +1.1 2,397,771 - +0.1 2,397,771 - +0.4 2,397,846 + -1.1 2,397,846 + +0.2 2,397,849 - +0.1 2,397,865 - +0.5 2,397,867 + +0.3 2,397,867 + -0.0 2,397,875 - +0.7 2,397,943 + -1.5 2,397,968 + -2.2 2,397,984 + -0.5 2,398,025 - -0.9 2,398,124 - CCNA_02250 0.14 +0.8
Or see this region's nucleotide sequence