Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02146 and CCNA_02147 are separated by 98 nucleotides CCNA_02147 and CCNA_02148 are separated by 74 nucleotides CCNA_02148 and CCNA_02149 are separated by 153 nucleotides
CCNA_02146: CCNA_02146 - hypothetical protein, at 2,303,983 to 2,304,447
_02146
CCNA_02147: CCNA_02147 - hypothetical protein, at 2,304,546 to 2,304,875
_02147
CCNA_02148: CCNA_02148 - hypothetical protein, at 2,304,950 to 2,305,180
_02148
CCNA_02149: CCNA_02149 - 1-deoxy-D-xylulose 5-phosphate synthase, at 2,305,334 to 2,307,256
_02149
Position (kb)
2304
2305
2306 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2303.954 kb on + strand at 2303.988 kb on + strand at 2304.004 kb on - strand at 2304.039 kb on - strand, within CCNA_02146 at 2304.041 kb on - strand, within CCNA_02146 at 2304.046 kb on + strand, within CCNA_02146 at 2304.046 kb on + strand, within CCNA_02146 at 2304.046 kb on + strand, within CCNA_02146 at 2304.053 kb on + strand, within CCNA_02146 at 2304.065 kb on - strand, within CCNA_02146 at 2304.093 kb on + strand, within CCNA_02146 at 2304.093 kb on + strand, within CCNA_02146 at 2304.100 kb on + strand, within CCNA_02146 at 2304.101 kb on - strand, within CCNA_02146 at 2304.103 kb on - strand, within CCNA_02146 at 2304.115 kb on + strand, within CCNA_02146 at 2304.115 kb on + strand, within CCNA_02146 at 2304.148 kb on + strand, within CCNA_02146 at 2304.158 kb on + strand, within CCNA_02146 at 2304.188 kb on - strand, within CCNA_02146 at 2304.228 kb on - strand, within CCNA_02146 at 2304.241 kb on - strand, within CCNA_02146 at 2304.246 kb on - strand, within CCNA_02146 at 2304.256 kb on - strand, within CCNA_02146 at 2304.257 kb on - strand, within CCNA_02146 at 2304.279 kb on - strand, within CCNA_02146 at 2304.281 kb on + strand, within CCNA_02146 at 2304.309 kb on + strand, within CCNA_02146 at 2304.358 kb on - strand, within CCNA_02146 at 2304.358 kb on - strand, within CCNA_02146 at 2304.358 kb on - strand, within CCNA_02146 at 2304.391 kb on - strand, within CCNA_02146 at 2304.426 kb on + strand at 2304.433 kb on - strand at 2304.450 kb on - strand at 2304.493 kb on + strand at 2304.592 kb on + strand, within CCNA_02147 at 2304.596 kb on + strand, within CCNA_02147 at 2304.614 kb on - strand, within CCNA_02147 at 2304.619 kb on - strand, within CCNA_02147 at 2304.628 kb on - strand, within CCNA_02147 at 2304.633 kb on - strand, within CCNA_02147 at 2304.653 kb on - strand, within CCNA_02147 at 2304.653 kb on - strand, within CCNA_02147 at 2304.653 kb on - strand, within CCNA_02147 at 2304.663 kb on - strand, within CCNA_02147 at 2304.665 kb on + strand, within CCNA_02147 at 2304.685 kb on - strand, within CCNA_02147 at 2304.698 kb on - strand, within CCNA_02147 at 2304.785 kb on + strand, within CCNA_02147 at 2304.787 kb on + strand, within CCNA_02147 at 2304.803 kb on - strand, within CCNA_02147 at 2304.833 kb on - strand, within CCNA_02147 at 2304.877 kb on - strand at 2304.877 kb on - strand at 2305.000 kb on + strand, within CCNA_02148 at 2305.004 kb on - strand, within CCNA_02148 at 2305.050 kb on + strand, within CCNA_02148 at 2305.050 kb on + strand, within CCNA_02148 at 2305.050 kb on + strand, within CCNA_02148 at 2305.057 kb on - strand, within CCNA_02148 at 2305.058 kb on - strand, within CCNA_02148 at 2305.098 kb on + strand, within CCNA_02148 at 2305.172 kb on - strand at 2305.196 kb on - strand at 2305.231 kb on + strand at 2305.231 kb on + strand at 2305.231 kb on + strand at 2305.238 kb on + strand at 2305.243 kb on + strand at 2305.243 kb on + strand at 2305.246 kb on - strand at 2305.251 kb on - strand at 2305.251 kb on - strand at 2305.260 kb on + strand at 2305.268 kb on - strand at 2305.268 kb on - strand at 2305.378 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,303,954 + -0.5 2,303,988 + -1.6 2,304,004 - -0.3 2,304,039 - CCNA_02146 0.12 -0.9 2,304,041 - CCNA_02146 0.12 +0.1 2,304,046 + CCNA_02146 0.14 -0.2 2,304,046 + CCNA_02146 0.14 +0.5 2,304,046 + CCNA_02146 0.14 -0.4 2,304,053 + CCNA_02146 0.15 +0.9 2,304,065 - CCNA_02146 0.18 +0.1 2,304,093 + CCNA_02146 0.24 -0.2 2,304,093 + CCNA_02146 0.24 +0.2 2,304,100 + CCNA_02146 0.25 +0.0 2,304,101 - CCNA_02146 0.25 -0.1 2,304,103 - CCNA_02146 0.26 -0.8 2,304,115 + CCNA_02146 0.28 +1.4 2,304,115 + CCNA_02146 0.28 +0.3 2,304,148 + CCNA_02146 0.35 -0.2 2,304,158 + CCNA_02146 0.38 -0.7 2,304,188 - CCNA_02146 0.44 -0.1 2,304,228 - CCNA_02146 0.53 -0.2 2,304,241 - CCNA_02146 0.55 -0.2 2,304,246 - CCNA_02146 0.57 +1.1 2,304,256 - CCNA_02146 0.59 +0.9 2,304,257 - CCNA_02146 0.59 +0.3 2,304,279 - CCNA_02146 0.64 -0.4 2,304,281 + CCNA_02146 0.64 +0.4 2,304,309 + CCNA_02146 0.70 -0.9 2,304,358 - CCNA_02146 0.81 +0.7 2,304,358 - CCNA_02146 0.81 -1.4 2,304,358 - CCNA_02146 0.81 +0.9 2,304,391 - CCNA_02146 0.88 -0.1 2,304,426 + +0.7 2,304,433 - -0.3 2,304,450 - -0.0 2,304,493 + -1.0 2,304,592 + CCNA_02147 0.14 -0.6 2,304,596 + CCNA_02147 0.15 -0.6 2,304,614 - CCNA_02147 0.21 -1.2 2,304,619 - CCNA_02147 0.22 -0.9 2,304,628 - CCNA_02147 0.25 -1.1 2,304,633 - CCNA_02147 0.26 -1.1 2,304,653 - CCNA_02147 0.32 -0.5 2,304,653 - CCNA_02147 0.32 -2.0 2,304,653 - CCNA_02147 0.32 +0.1 2,304,663 - CCNA_02147 0.35 -0.3 2,304,665 + CCNA_02147 0.36 +0.2 2,304,685 - CCNA_02147 0.42 -0.8 2,304,698 - CCNA_02147 0.46 -0.4 2,304,785 + CCNA_02147 0.72 +2.1 2,304,787 + CCNA_02147 0.73 +0.8 2,304,803 - CCNA_02147 0.78 +1.1 2,304,833 - CCNA_02147 0.87 +0.2 2,304,877 - -0.0 2,304,877 - +0.1 2,305,000 + CCNA_02148 0.22 +0.8 2,305,004 - CCNA_02148 0.23 -0.0 2,305,050 + CCNA_02148 0.43 +0.5 2,305,050 + CCNA_02148 0.43 +1.1 2,305,050 + CCNA_02148 0.43 +0.1 2,305,057 - CCNA_02148 0.46 +0.2 2,305,058 - CCNA_02148 0.47 -0.4 2,305,098 + CCNA_02148 0.64 +1.1 2,305,172 - -0.1 2,305,196 - +0.3 2,305,231 + +0.8 2,305,231 + +0.4 2,305,231 + +0.5 2,305,238 + -1.6 2,305,243 + +0.8 2,305,243 + -0.3 2,305,246 - +0.2 2,305,251 - -0.1 2,305,251 - +0.7 2,305,260 + -0.1 2,305,268 - +0.6 2,305,268 - +0.2 2,305,378 - +0.4
Or see this region's nucleotide sequence