Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02065 and CCNA_02066 are separated by 134 nucleotides CCNA_02066 and CCNA_02067 are separated by 71 nucleotides CCNA_02067 and CCNA_02068 are separated by 95 nucleotides CCNA_02068 and CCNA_02069 overlap by 1 nucleotides
CCNA_02065: CCNA_02065 - acylamino-acid-releasing enzyme, at 2,215,780 to 2,217,882
_02065
CCNA_02066: CCNA_02066 - phytoene/squalene synthase family protein, at 2,218,017 to 2,218,730
_02066
CCNA_02067: CCNA_02067 - conserved membrane spanning protein, at 2,218,802 to 2,219,416
_02067
CCNA_02068: CCNA_02068 - Mth938-like membrane protein, at 2,219,512 to 2,219,865
_02068
CCNA_02069: CCNA_02069 - protein translocase subunit secF, at 2,219,865 to 2,220,830
_02069
Position (kb)
2218
2219
2220 Strain fitness (log2 ratio)
-1
0
1 at 2217.802 kb on + strand at 2217.810 kb on - strand at 2217.822 kb on + strand at 2217.900 kb on + strand at 2217.908 kb on - strand at 2217.944 kb on - strand at 2217.959 kb on + strand at 2217.975 kb on - strand at 2217.999 kb on + strand at 2218.168 kb on + strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.176 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.478 kb on - strand, within CCNA_02066 at 2218.553 kb on - strand, within CCNA_02066 at 2218.675 kb on - strand at 2218.680 kb on + strand at 2218.726 kb on + strand at 2218.840 kb on + strand at 2218.866 kb on - strand, within CCNA_02067 at 2218.902 kb on + strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.904 kb on - strand, within CCNA_02067 at 2218.973 kb on - strand, within CCNA_02067 at 2218.997 kb on + strand, within CCNA_02067 at 2219.010 kb on - strand, within CCNA_02067 at 2219.018 kb on + strand, within CCNA_02067 at 2219.018 kb on + strand, within CCNA_02067 at 2219.049 kb on - strand, within CCNA_02067 at 2219.073 kb on - strand, within CCNA_02067 at 2219.075 kb on - strand, within CCNA_02067 at 2219.126 kb on + strand, within CCNA_02067 at 2219.153 kb on - strand, within CCNA_02067 at 2219.249 kb on - strand, within CCNA_02067 at 2219.368 kb on - strand at 2219.528 kb on + strand at 2219.554 kb on + strand, within CCNA_02068 at 2219.562 kb on - strand, within CCNA_02068 at 2219.588 kb on + strand, within CCNA_02068 at 2219.627 kb on - strand, within CCNA_02068 at 2219.654 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.659 kb on + strand, within CCNA_02068 at 2219.724 kb on + strand, within CCNA_02068
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,217,802 + +0.2 2,217,810 - +0.1 2,217,822 + -0.1 2,217,900 + +0.8 2,217,908 - -0.9 2,217,944 - -0.3 2,217,959 + -0.8 2,217,975 - -0.2 2,217,999 + +0.4 2,218,168 + CCNA_02066 0.21 -0.5 2,218,176 - CCNA_02066 0.22 +0.0 2,218,176 - CCNA_02066 0.22 +0.2 2,218,478 - CCNA_02066 0.65 -0.5 2,218,478 - CCNA_02066 0.65 +1.0 2,218,478 - CCNA_02066 0.65 +0.5 2,218,553 - CCNA_02066 0.75 -0.6 2,218,675 - -0.6 2,218,680 + -0.2 2,218,726 + +0.2 2,218,840 + -0.7 2,218,866 - CCNA_02067 0.10 -0.2 2,218,902 + CCNA_02067 0.16 -0.1 2,218,904 - CCNA_02067 0.17 +0.7 2,218,904 - CCNA_02067 0.17 +0.9 2,218,973 - CCNA_02067 0.28 +0.7 2,218,997 + CCNA_02067 0.32 -0.1 2,219,010 - CCNA_02067 0.34 +0.7 2,219,018 + CCNA_02067 0.35 -0.8 2,219,018 + CCNA_02067 0.35 +0.8 2,219,049 - CCNA_02067 0.40 +1.3 2,219,073 - CCNA_02067 0.44 -0.5 2,219,075 - CCNA_02067 0.44 +0.4 2,219,126 + CCNA_02067 0.53 -0.1 2,219,153 - CCNA_02067 0.57 +0.5 2,219,249 - CCNA_02067 0.73 +0.9 2,219,368 - -0.7 2,219,528 + +0.5 2,219,554 + CCNA_02068 0.12 -0.4 2,219,562 - CCNA_02068 0.14 +0.1 2,219,588 + CCNA_02068 0.21 -0.4 2,219,627 - CCNA_02068 0.32 +0.5 2,219,654 + CCNA_02068 0.40 -0.9 2,219,659 + CCNA_02068 0.42 -1.2 2,219,659 + CCNA_02068 0.42 +0.3 2,219,724 + CCNA_02068 0.60 -0.0
Or see this region's nucleotide sequence