Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02059 and CCNA_02060 overlap by 4 nucleotides CCNA_02060 and CCNA_02061 are separated by 163 nucleotides
CCNA_02059: CCNA_02059 - ribosomal large subunit pseudouridine synthase C, at 2,208,899 to 2,209,894
_02059
CCNA_02060: CCNA_02060 - CrcB family protein, at 2,209,891 to 2,210,274
_02060
CCNA_02061: CCNA_02061 - hypothetical protein, at 2,210,438 to 2,211,667
_02061
Position (kb)
2209
2210
2211 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2209.037 kb on - strand, within CCNA_02059 at 2209.067 kb on + strand, within CCNA_02059 at 2209.074 kb on - strand, within CCNA_02059 at 2209.077 kb on + strand, within CCNA_02059 at 2209.126 kb on - strand, within CCNA_02059 at 2209.165 kb on - strand, within CCNA_02059 at 2209.165 kb on - strand, within CCNA_02059 at 2209.167 kb on - strand, within CCNA_02059 at 2209.187 kb on - strand, within CCNA_02059 at 2209.271 kb on - strand, within CCNA_02059 at 2209.296 kb on - strand, within CCNA_02059 at 2209.345 kb on + strand, within CCNA_02059 at 2209.353 kb on - strand, within CCNA_02059 at 2209.379 kb on + strand, within CCNA_02059 at 2209.441 kb on - strand, within CCNA_02059 at 2209.442 kb on - strand, within CCNA_02059 at 2209.452 kb on - strand, within CCNA_02059 at 2209.503 kb on + strand, within CCNA_02059 at 2209.518 kb on - strand, within CCNA_02059 at 2209.523 kb on - strand, within CCNA_02059 at 2209.546 kb on + strand, within CCNA_02059 at 2209.586 kb on - strand, within CCNA_02059 at 2209.586 kb on - strand, within CCNA_02059 at 2209.662 kb on - strand, within CCNA_02059 at 2209.736 kb on - strand, within CCNA_02059 at 2209.736 kb on - strand, within CCNA_02059 at 2209.799 kb on + strand at 2209.807 kb on - strand at 2209.922 kb on - strand at 2210.007 kb on + strand, within CCNA_02060 at 2210.093 kb on - strand, within CCNA_02060 at 2210.093 kb on - strand, within CCNA_02060 at 2210.277 kb on - strand at 2210.304 kb on - strand at 2210.359 kb on - strand at 2210.416 kb on - strand at 2210.428 kb on - strand at 2210.441 kb on + strand at 2210.443 kb on + strand at 2210.459 kb on - strand at 2210.514 kb on + strand at 2210.553 kb on + strand at 2210.553 kb on + strand at 2210.553 kb on + strand at 2210.617 kb on - strand, within CCNA_02061 at 2210.617 kb on - strand, within CCNA_02061 at 2210.703 kb on + strand, within CCNA_02061 at 2210.708 kb on + strand, within CCNA_02061 at 2210.778 kb on - strand, within CCNA_02061 at 2210.837 kb on - strand, within CCNA_02061 at 2210.839 kb on + strand, within CCNA_02061 at 2210.839 kb on + strand, within CCNA_02061 at 2210.839 kb on + strand, within CCNA_02061 at 2210.847 kb on - strand, within CCNA_02061 at 2210.847 kb on - strand, within CCNA_02061 at 2210.847 kb on - strand, within CCNA_02061 at 2210.862 kb on - strand, within CCNA_02061 at 2211.068 kb on - strand, within CCNA_02061 at 2211.086 kb on + strand, within CCNA_02061 at 2211.111 kb on - strand, within CCNA_02061 at 2211.111 kb on - strand, within CCNA_02061 at 2211.146 kb on + strand, within CCNA_02061 at 2211.154 kb on - strand, within CCNA_02061 at 2211.154 kb on - strand, within CCNA_02061 at 2211.154 kb on - strand, within CCNA_02061 at 2211.198 kb on - strand, within CCNA_02061 at 2211.200 kb on + strand, within CCNA_02061 at 2211.264 kb on - strand, within CCNA_02061
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,209,037 - CCNA_02059 0.14 +1.1 2,209,067 + CCNA_02059 0.17 -1.4 2,209,074 - CCNA_02059 0.18 +1.9 2,209,077 + CCNA_02059 0.18 -0.8 2,209,126 - CCNA_02059 0.23 -0.2 2,209,165 - CCNA_02059 0.27 -0.5 2,209,165 - CCNA_02059 0.27 +0.1 2,209,167 - CCNA_02059 0.27 -0.0 2,209,187 - CCNA_02059 0.29 +0.3 2,209,271 - CCNA_02059 0.37 +0.4 2,209,296 - CCNA_02059 0.40 -0.2 2,209,345 + CCNA_02059 0.45 -0.6 2,209,353 - CCNA_02059 0.46 +0.4 2,209,379 + CCNA_02059 0.48 +0.6 2,209,441 - CCNA_02059 0.54 +0.3 2,209,442 - CCNA_02059 0.55 -0.5 2,209,452 - CCNA_02059 0.56 +1.4 2,209,503 + CCNA_02059 0.61 -0.0 2,209,518 - CCNA_02059 0.62 -0.2 2,209,523 - CCNA_02059 0.63 +0.4 2,209,546 + CCNA_02059 0.65 -2.4 2,209,586 - CCNA_02059 0.69 +1.4 2,209,586 - CCNA_02059 0.69 +0.8 2,209,662 - CCNA_02059 0.77 +0.4 2,209,736 - CCNA_02059 0.84 -0.6 2,209,736 - CCNA_02059 0.84 +1.2 2,209,799 + -1.0 2,209,807 - +0.1 2,209,922 - +0.8 2,210,007 + CCNA_02060 0.30 -1.7 2,210,093 - CCNA_02060 0.53 +0.8 2,210,093 - CCNA_02060 0.53 -0.6 2,210,277 - +0.2 2,210,304 - -0.8 2,210,359 - +0.2 2,210,416 - +0.2 2,210,428 - -2.1 2,210,441 + +2.5 2,210,443 + +1.3 2,210,459 - -0.8 2,210,514 + +0.4 2,210,553 + -1.5 2,210,553 + -0.2 2,210,553 + +0.2 2,210,617 - CCNA_02061 0.15 -1.1 2,210,617 - CCNA_02061 0.15 +0.4 2,210,703 + CCNA_02061 0.22 +0.0 2,210,708 + CCNA_02061 0.22 +0.4 2,210,778 - CCNA_02061 0.28 +0.8 2,210,837 - CCNA_02061 0.32 +0.2 2,210,839 + CCNA_02061 0.33 +0.6 2,210,839 + CCNA_02061 0.33 +0.8 2,210,839 + CCNA_02061 0.33 +0.3 2,210,847 - CCNA_02061 0.33 -2.3 2,210,847 - CCNA_02061 0.33 -0.2 2,210,847 - CCNA_02061 0.33 +0.6 2,210,862 - CCNA_02061 0.34 +0.1 2,211,068 - CCNA_02061 0.51 -0.1 2,211,086 + CCNA_02061 0.53 +0.1 2,211,111 - CCNA_02061 0.55 -0.3 2,211,111 - CCNA_02061 0.55 -0.6 2,211,146 + CCNA_02061 0.58 +0.4 2,211,154 - CCNA_02061 0.58 +0.3 2,211,154 - CCNA_02061 0.58 +1.3 2,211,154 - CCNA_02061 0.58 +0.2 2,211,198 - CCNA_02061 0.62 -1.6 2,211,200 + CCNA_02061 0.62 +0.7 2,211,264 - CCNA_02061 0.67 -0.5
Or see this region's nucleotide sequence