Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01930 and CCNA_01931 are separated by 94 nucleotides CCNA_01931 and CCNA_01932 overlap by 4 nucleotides CCNA_01932 and CCNA_01933 are separated by 104 nucleotides CCNA_01933 and CCNA_01934 overlap by 1 nucleotides CCNA_01934 and CCNA_01935 are separated by 5 nucleotides
CCNA_01930: CCNA_01930 - ADP-heptose LPS heptosyltransferase, at 2,075,420 to 2,076,391
_01930
CCNA_01931: CCNA_01931 - TetR-family transcriptional regulator, at 2,076,486 to 2,077,046
_01931
CCNA_01932: CCNA_01932 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 2,077,043 to 2,077,861
_01932
CCNA_01933: CCNA_01933 - hypothetical protein, at 2,077,966 to 2,078,166
_01933
CCNA_01934: CCNA_01934 - HesB protein family, at 2,078,166 to 2,078,567
_01934
CCNA_01935: CCNA_01935 - FeS assembly SUF system protein, at 2,078,573 to 2,078,929
_01935
Position (kb)
2077
2078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2076.127 kb on + strand, within CCNA_01930 at 2076.151 kb on + strand, within CCNA_01930 at 2076.159 kb on - strand, within CCNA_01930 at 2076.202 kb on + strand, within CCNA_01930 at 2076.244 kb on + strand, within CCNA_01930 at 2076.244 kb on + strand, within CCNA_01930 at 2076.258 kb on + strand, within CCNA_01930 at 2076.258 kb on + strand, within CCNA_01930 at 2076.266 kb on - strand, within CCNA_01930 at 2076.313 kb on + strand at 2076.386 kb on + strand at 2076.595 kb on + strand, within CCNA_01931 at 2076.625 kb on - strand, within CCNA_01931 at 2076.628 kb on + strand, within CCNA_01931 at 2076.667 kb on + strand, within CCNA_01931 at 2076.667 kb on + strand, within CCNA_01931 at 2076.672 kb on + strand, within CCNA_01931 at 2076.673 kb on - strand, within CCNA_01931 at 2076.707 kb on + strand, within CCNA_01931 at 2076.721 kb on + strand, within CCNA_01931 at 2076.731 kb on + strand, within CCNA_01931 at 2076.731 kb on + strand, within CCNA_01931 at 2076.739 kb on - strand, within CCNA_01931 at 2076.739 kb on - strand, within CCNA_01931 at 2076.797 kb on + strand, within CCNA_01931 at 2076.931 kb on - strand, within CCNA_01931 at 2076.934 kb on + strand, within CCNA_01931 at 2076.938 kb on + strand, within CCNA_01931 at 2076.938 kb on + strand, within CCNA_01931 at 2076.948 kb on + strand, within CCNA_01931 at 2076.948 kb on + strand, within CCNA_01931 at 2076.953 kb on + strand, within CCNA_01931 at 2076.953 kb on + strand, within CCNA_01931 at 2076.953 kb on + strand, within CCNA_01931 at 2076.953 kb on + strand, within CCNA_01931 at 2076.953 kb on + strand, within CCNA_01931 at 2076.956 kb on - strand, within CCNA_01931 at 2076.956 kb on - strand, within CCNA_01931 at 2076.960 kb on + strand, within CCNA_01931 at 2076.961 kb on - strand, within CCNA_01931 at 2077.010 kb on - strand at 2077.019 kb on + strand at 2077.027 kb on - strand at 2077.160 kb on + strand, within CCNA_01932 at 2077.161 kb on + strand, within CCNA_01932 at 2077.185 kb on + strand, within CCNA_01932 at 2077.199 kb on + strand, within CCNA_01932 at 2077.207 kb on - strand, within CCNA_01932 at 2077.218 kb on + strand, within CCNA_01932 at 2077.226 kb on + strand, within CCNA_01932 at 2077.231 kb on + strand, within CCNA_01932 at 2077.231 kb on + strand, within CCNA_01932 at 2077.234 kb on - strand, within CCNA_01932 at 2077.239 kb on - strand, within CCNA_01932 at 2077.280 kb on + strand, within CCNA_01932 at 2077.280 kb on + strand, within CCNA_01932 at 2077.288 kb on + strand, within CCNA_01932 at 2077.337 kb on - strand, within CCNA_01932 at 2077.380 kb on - strand, within CCNA_01932 at 2077.433 kb on - strand, within CCNA_01932 at 2077.447 kb on + strand, within CCNA_01932 at 2077.447 kb on + strand, within CCNA_01932 at 2077.447 kb on + strand, within CCNA_01932 at 2077.455 kb on - strand, within CCNA_01932 at 2077.455 kb on - strand, within CCNA_01932 at 2077.455 kb on - strand, within CCNA_01932 at 2077.455 kb on - strand, within CCNA_01932 at 2077.455 kb on - strand, within CCNA_01932 at 2077.482 kb on + strand, within CCNA_01932 at 2077.482 kb on + strand, within CCNA_01932 at 2077.487 kb on + strand, within CCNA_01932 at 2077.495 kb on - strand, within CCNA_01932 at 2077.561 kb on - strand, within CCNA_01932 at 2077.609 kb on - strand, within CCNA_01932 at 2077.609 kb on - strand, within CCNA_01932 at 2077.676 kb on - strand, within CCNA_01932 at 2077.678 kb on + strand, within CCNA_01932 at 2077.720 kb on + strand, within CCNA_01932 at 2077.750 kb on + strand, within CCNA_01932 at 2077.788 kb on - strand at 2077.790 kb on - strand at 2077.880 kb on - strand at 2077.892 kb on - strand at 2077.892 kb on - strand at 2077.931 kb on + strand at 2077.934 kb on - strand at 2077.939 kb on - strand at 2077.942 kb on + strand at 2077.950 kb on + strand at 2077.950 kb on - strand at 2077.952 kb on - strand at 2078.103 kb on - strand, within CCNA_01933 at 2078.103 kb on - strand, within CCNA_01933 at 2078.106 kb on - strand, within CCNA_01933 at 2078.110 kb on - strand, within CCNA_01933 at 2078.120 kb on + strand, within CCNA_01933 at 2078.128 kb on - strand, within CCNA_01933 at 2078.128 kb on - strand, within CCNA_01933 at 2078.136 kb on + strand, within CCNA_01933 at 2078.164 kb on + strand at 2078.169 kb on + strand at 2078.169 kb on + strand at 2078.169 kb on + strand at 2078.172 kb on - strand at 2078.177 kb on - strand at 2078.177 kb on - strand at 2078.177 kb on - strand at 2078.177 kb on - strand at 2078.178 kb on - strand at 2078.262 kb on - strand, within CCNA_01934 at 2078.296 kb on + strand, within CCNA_01934 at 2078.338 kb on + strand, within CCNA_01934 at 2078.443 kb on + strand, within CCNA_01934 at 2078.443 kb on + strand, within CCNA_01934 at 2078.443 kb on + strand, within CCNA_01934 at 2078.448 kb on + strand, within CCNA_01934 at 2078.448 kb on + strand, within CCNA_01934 at 2078.456 kb on - strand, within CCNA_01934 at 2078.481 kb on - strand, within CCNA_01934 at 2078.502 kb on - strand, within CCNA_01934 at 2078.561 kb on + strand at 2078.592 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 2,076,127 + CCNA_01930 0.73 +0.2 2,076,151 + CCNA_01930 0.75 +0.1 2,076,159 - CCNA_01930 0.76 +0.2 2,076,202 + CCNA_01930 0.80 +2.0 2,076,244 + CCNA_01930 0.85 -1.4 2,076,244 + CCNA_01930 0.85 -0.6 2,076,258 + CCNA_01930 0.86 -0.2 2,076,258 + CCNA_01930 0.86 -0.0 2,076,266 - CCNA_01930 0.87 -0.2 2,076,313 + -0.3 2,076,386 + -0.3 2,076,595 + CCNA_01931 0.19 +0.3 2,076,625 - CCNA_01931 0.25 -1.9 2,076,628 + CCNA_01931 0.25 +0.5 2,076,667 + CCNA_01931 0.32 +0.0 2,076,667 + CCNA_01931 0.32 +0.6 2,076,672 + CCNA_01931 0.33 +0.9 2,076,673 - CCNA_01931 0.33 -0.8 2,076,707 + CCNA_01931 0.39 +0.7 2,076,721 + CCNA_01931 0.42 +0.5 2,076,731 + CCNA_01931 0.44 +0.3 2,076,731 + CCNA_01931 0.44 +0.7 2,076,739 - CCNA_01931 0.45 -1.5 2,076,739 - CCNA_01931 0.45 -1.4 2,076,797 + CCNA_01931 0.55 +0.7 2,076,931 - CCNA_01931 0.79 -1.2 2,076,934 + CCNA_01931 0.80 +0.4 2,076,938 + CCNA_01931 0.81 +0.6 2,076,938 + CCNA_01931 0.81 +0.8 2,076,948 + CCNA_01931 0.82 +0.9 2,076,948 + CCNA_01931 0.82 +0.6 2,076,953 + CCNA_01931 0.83 +1.1 2,076,953 + CCNA_01931 0.83 +0.5 2,076,953 + CCNA_01931 0.83 +1.8 2,076,953 + CCNA_01931 0.83 +0.4 2,076,953 + CCNA_01931 0.83 +1.0 2,076,956 - CCNA_01931 0.84 -1.1 2,076,956 - CCNA_01931 0.84 +0.0 2,076,960 + CCNA_01931 0.84 +0.6 2,076,961 - CCNA_01931 0.85 -0.2 2,077,010 - -0.7 2,077,019 + +1.2 2,077,027 - -1.5 2,077,160 + CCNA_01932 0.14 +1.8 2,077,161 + CCNA_01932 0.14 -1.7 2,077,185 + CCNA_01932 0.17 -1.6 2,077,199 + CCNA_01932 0.19 +0.8 2,077,207 - CCNA_01932 0.20 -0.3 2,077,218 + CCNA_01932 0.21 +0.0 2,077,226 + CCNA_01932 0.22 +0.8 2,077,231 + CCNA_01932 0.23 -0.7 2,077,231 + CCNA_01932 0.23 -0.6 2,077,234 - CCNA_01932 0.23 -0.5 2,077,239 - CCNA_01932 0.24 +0.4 2,077,280 + CCNA_01932 0.29 -0.9 2,077,280 + CCNA_01932 0.29 -0.3 2,077,288 + CCNA_01932 0.30 +0.1 2,077,337 - CCNA_01932 0.36 -0.1 2,077,380 - CCNA_01932 0.41 -1.3 2,077,433 - CCNA_01932 0.48 -2.8 2,077,447 + CCNA_01932 0.49 +1.0 2,077,447 + CCNA_01932 0.49 -1.0 2,077,447 + CCNA_01932 0.49 -0.5 2,077,455 - CCNA_01932 0.50 -0.0 2,077,455 - CCNA_01932 0.50 +0.3 2,077,455 - CCNA_01932 0.50 -1.2 2,077,455 - CCNA_01932 0.50 +0.3 2,077,455 - CCNA_01932 0.50 +0.0 2,077,482 + CCNA_01932 0.54 +0.2 2,077,482 + CCNA_01932 0.54 +1.2 2,077,487 + CCNA_01932 0.54 -1.2 2,077,495 - CCNA_01932 0.55 -0.9 2,077,561 - CCNA_01932 0.63 -1.3 2,077,609 - CCNA_01932 0.69 +0.1 2,077,609 - CCNA_01932 0.69 +0.1 2,077,676 - CCNA_01932 0.77 +1.0 2,077,678 + CCNA_01932 0.78 -0.0 2,077,720 + CCNA_01932 0.83 -0.1 2,077,750 + CCNA_01932 0.86 +1.1 2,077,788 - +0.4 2,077,790 - +0.9 2,077,880 - -0.0 2,077,892 - -0.1 2,077,892 - +1.5 2,077,931 + +2.4 2,077,934 - -0.9 2,077,939 - +1.1 2,077,942 + -0.0 2,077,950 + -0.1 2,077,950 - +0.5 2,077,952 - +0.6 2,078,103 - CCNA_01933 0.68 +0.6 2,078,103 - CCNA_01933 0.68 -0.5 2,078,106 - CCNA_01933 0.70 +0.5 2,078,110 - CCNA_01933 0.72 +0.5 2,078,120 + CCNA_01933 0.77 +0.0 2,078,128 - CCNA_01933 0.81 -1.6 2,078,128 - CCNA_01933 0.81 +0.3 2,078,136 + CCNA_01933 0.85 -0.2 2,078,164 + -0.3 2,078,169 + +0.5 2,078,169 + -0.4 2,078,169 + -0.4 2,078,172 - +0.7 2,078,177 - -0.2 2,078,177 - -0.0 2,078,177 - +1.7 2,078,177 - +0.5 2,078,178 - +0.1 2,078,262 - CCNA_01934 0.24 +0.7 2,078,296 + CCNA_01934 0.32 -1.2 2,078,338 + CCNA_01934 0.43 -0.5 2,078,443 + CCNA_01934 0.69 +0.5 2,078,443 + CCNA_01934 0.69 +0.1 2,078,443 + CCNA_01934 0.69 -0.0 2,078,448 + CCNA_01934 0.70 +0.5 2,078,448 + CCNA_01934 0.70 -1.2 2,078,456 - CCNA_01934 0.72 +0.4 2,078,481 - CCNA_01934 0.78 -0.1 2,078,502 - CCNA_01934 0.84 -0.2 2,078,561 + -1.5 2,078,592 + +1.0
Or see this region's nucleotide sequence