Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00974 and CCNA_00976 are separated by 446 nucleotides CCNA_00976 and CCNA_R0021 are separated by 173 nucleotides CCNA_R0021 and CCNA_R0022 are separated by 164 nucleotides CCNA_R0022 and CCNA_00977 are separated by 94 nucleotides
CCNA_00974: CCNA_00974 - OAR protein precursor, at 1,051,388 to 1,054,555
_00974
CCNA_00976: CCNA_00976 - signal peptide peptidase A, at 1,055,002 to 1,056,786
_00976
CCNA_R0021: CCNA_R0021 - tRNA-Pro, at 1,056,960 to 1,057,036
_R0021
CCNA_R0022: CCNA_R0022 - tRNA-Gln, at 1,057,201 to 1,057,274
_R0022
CCNA_00977: CCNA_00977 - transposase, IS4/5 family, at 1,057,369 to 1,057,779
_00977
Position (kb)
1055
1056
1057 Strain fitness (log2 ratio)
-2
-1
0
1 at 1054.046 kb on - strand, within CCNA_00974 at 1054.081 kb on + strand, within CCNA_00974 at 1054.094 kb on - strand, within CCNA_00974 at 1054.115 kb on - strand, within CCNA_00974 at 1054.187 kb on - strand, within CCNA_00974 at 1054.208 kb on - strand, within CCNA_00974 at 1054.210 kb on + strand, within CCNA_00974 at 1054.218 kb on - strand, within CCNA_00974 at 1054.218 kb on - strand, within CCNA_00974 at 1054.218 kb on - strand, within CCNA_00974 at 1054.262 kb on + strand at 1054.276 kb on - strand at 1054.314 kb on - strand at 1054.329 kb on + strand at 1054.337 kb on - strand at 1054.368 kb on + strand at 1054.472 kb on + strand at 1054.514 kb on + strand at 1054.535 kb on + strand at 1054.543 kb on - strand at 1054.556 kb on + strand at 1054.564 kb on - strand at 1054.589 kb on + strand at 1054.600 kb on - strand at 1054.632 kb on + strand at 1054.632 kb on + strand at 1054.634 kb on - strand at 1054.725 kb on - strand at 1054.731 kb on + strand at 1054.832 kb on + strand at 1054.853 kb on + strand at 1054.861 kb on + strand at 1054.866 kb on + strand at 1054.894 kb on - strand at 1054.911 kb on + strand at 1054.934 kb on + strand at 1055.391 kb on - strand, within CCNA_00976 at 1055.446 kb on - strand, within CCNA_00976 at 1055.526 kb on - strand, within CCNA_00976 at 1055.577 kb on - strand, within CCNA_00976 at 1055.765 kb on + strand, within CCNA_00976 at 1056.077 kb on + strand, within CCNA_00976 at 1056.132 kb on - strand, within CCNA_00976 at 1056.350 kb on - strand, within CCNA_00976 at 1056.400 kb on - strand, within CCNA_00976 at 1056.785 kb on - strand at 1056.849 kb on - strand at 1056.849 kb on - strand at 1057.315 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 1,054,046 - CCNA_00974 0.84 +0.1 1,054,081 + CCNA_00974 0.85 +0.4 1,054,094 - CCNA_00974 0.85 +1.0 1,054,115 - CCNA_00974 0.86 +0.7 1,054,187 - CCNA_00974 0.88 -1.9 1,054,208 - CCNA_00974 0.89 -1.4 1,054,210 + CCNA_00974 0.89 +0.9 1,054,218 - CCNA_00974 0.89 +0.4 1,054,218 - CCNA_00974 0.89 -1.1 1,054,218 - CCNA_00974 0.89 -0.2 1,054,262 + -0.1 1,054,276 - +0.3 1,054,314 - +0.2 1,054,329 + +0.2 1,054,337 - -0.0 1,054,368 + +0.8 1,054,472 + -2.1 1,054,514 + +0.1 1,054,535 + +0.1 1,054,543 - -0.7 1,054,556 + +0.9 1,054,564 - +1.6 1,054,589 + +0.8 1,054,600 - -0.0 1,054,632 + +0.3 1,054,632 + -0.4 1,054,634 - +0.4 1,054,725 - +1.6 1,054,731 + +0.4 1,054,832 + +0.7 1,054,853 + +0.9 1,054,861 + -0.2 1,054,866 + +1.0 1,054,894 - +0.5 1,054,911 + +0.3 1,054,934 + +1.1 1,055,391 - CCNA_00976 0.22 -0.4 1,055,446 - CCNA_00976 0.25 -1.2 1,055,526 - CCNA_00976 0.29 +0.8 1,055,577 - CCNA_00976 0.32 +1.2 1,055,765 + CCNA_00976 0.43 -1.2 1,056,077 + CCNA_00976 0.60 +0.8 1,056,132 - CCNA_00976 0.63 +0.8 1,056,350 - CCNA_00976 0.76 +0.2 1,056,400 - CCNA_00976 0.78 -0.7 1,056,785 - +1.7 1,056,849 - -1.9 1,056,849 - -1.1 1,057,315 + +0.2
Or see this region's nucleotide sequence