Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00892 and CCNA_00893 are separated by 159 nucleotides CCNA_00893 and CCNA_00894 are separated by 181 nucleotides
CCNA_00892: CCNA_00892 - phosphoenolpyruvate-protein phosphotransferase PtsP, at 970,314 to 972,578
_00892
CCNA_00893: CCNA_00893 - helix-turn-helix DNA-binding protein, at 972,738 to 973,802
_00893
CCNA_00894: CCNA_00894 - 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase, at 973,984 to 975,135
_00894
Position (kb) 972
973
974 Strain fitness (log2 ratio) -2
-1
0
1 at 971.744 kb on + strand, within CCNA_00892 at 971.945 kb on - strand, within CCNA_00892 at 972.224 kb on + strand, within CCNA_00892 at 972.232 kb on - strand, within CCNA_00892 at 972.539 kb on + strand at 972.548 kb on - strand at 972.560 kb on + strand at 972.568 kb on - strand at 972.667 kb on + strand at 972.678 kb on + strand at 972.678 kb on + strand at 972.678 kb on + strand at 972.686 kb on - strand at 972.686 kb on - strand at 972.686 kb on - strand at 972.755 kb on - strand at 972.766 kb on - strand at 973.393 kb on + strand, within CCNA_00893 at 973.481 kb on + strand, within CCNA_00893 at 973.523 kb on + strand, within CCNA_00893 at 973.523 kb on + strand, within CCNA_00893 at 973.637 kb on + strand, within CCNA_00893 at 973.747 kb on - strand at 973.748 kb on + strand at 973.752 kb on - strand at 973.756 kb on - strand at 973.756 kb on - strand at 973.756 kb on - strand at 973.942 kb on - strand at 973.948 kb on + strand at 973.948 kb on + strand at 973.956 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 971,744 + CCNA_00892 0.63 +0.8 971,945 - CCNA_00892 0.72 -1.4 972,224 + CCNA_00892 0.84 -0.7 972,232 - CCNA_00892 0.85 -0.8 972,539 + -0.3 972,548 - +0.8 972,560 + +0.3 972,568 - +0.4 972,667 + -0.0 972,678 + +0.7 972,678 + +0.7 972,678 + -0.0 972,686 - -1.7 972,686 - -0.2 972,686 - +0.3 972,755 - -1.1 972,766 - +0.8 973,393 + CCNA_00893 0.62 -0.4 973,481 + CCNA_00893 0.70 -1.1 973,523 + CCNA_00893 0.74 -2.0 973,523 + CCNA_00893 0.74 -1.4 973,637 + CCNA_00893 0.84 -0.0 973,747 - +0.2 973,748 + -1.8 973,752 - -1.4 973,756 - -1.0 973,756 - -0.0 973,756 - -0.0 973,942 - -0.2 973,948 + +0.8 973,948 + +0.0 973,956 - +0.3
Or see this region's nucleotide sequence