Experiment: PYE with CHIR-090 40 ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00130 and CCNA_00131 are separated by 200 nucleotides CCNA_00131 and CCNA_00132 are separated by 91 nucleotides
CCNA_00130: CCNA_00130 - Kup system potassium uptake protein, at 138,921 to 140,918
_00130
CCNA_00131: CCNA_00131 - Rrf2 family protein, at 141,119 to 141,541
_00131
CCNA_00132: CCNA_00132 - ketoacyl reductase hetN, at 141,633 to 142,433
_00132
Position (kb) 141
142 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 140.903 kb on - strand at 140.925 kb on - strand at 140.925 kb on - strand at 140.933 kb on - strand at 140.933 kb on - strand at 140.952 kb on - strand at 140.989 kb on - strand at 141.022 kb on + strand at 141.022 kb on + strand at 141.035 kb on - strand at 141.048 kb on + strand at 141.072 kb on + strand at 141.099 kb on + strand at 141.155 kb on + strand at 141.160 kb on + strand at 141.160 kb on + strand at 141.163 kb on - strand, within CCNA_00131 at 141.184 kb on + strand, within CCNA_00131 at 141.213 kb on - strand, within CCNA_00131 at 141.218 kb on - strand, within CCNA_00131 at 141.233 kb on - strand, within CCNA_00131 at 141.233 kb on - strand, within CCNA_00131 at 141.243 kb on - strand, within CCNA_00131 at 141.259 kb on + strand, within CCNA_00131 at 141.264 kb on + strand, within CCNA_00131 at 141.274 kb on + strand, within CCNA_00131 at 141.274 kb on + strand, within CCNA_00131 at 141.274 kb on + strand, within CCNA_00131 at 141.282 kb on - strand, within CCNA_00131 at 141.282 kb on - strand, within CCNA_00131 at 141.292 kb on - strand, within CCNA_00131 at 141.307 kb on + strand, within CCNA_00131 at 141.328 kb on + strand, within CCNA_00131 at 141.328 kb on + strand, within CCNA_00131 at 141.328 kb on + strand, within CCNA_00131 at 141.364 kb on + strand, within CCNA_00131 at 141.372 kb on - strand, within CCNA_00131 at 141.374 kb on + strand, within CCNA_00131 at 141.382 kb on - strand, within CCNA_00131 at 141.417 kb on - strand, within CCNA_00131 at 141.481 kb on + strand, within CCNA_00131 at 141.489 kb on - strand, within CCNA_00131 at 141.505 kb on + strand at 141.531 kb on + strand at 141.531 kb on + strand at 141.531 kb on + strand at 141.531 kb on + strand at 141.533 kb on + strand at 141.539 kb on - strand at 141.539 kb on - strand at 141.539 kb on - strand at 141.539 kb on - strand at 141.591 kb on - strand at 141.613 kb on - strand at 141.638 kb on - strand at 141.648 kb on + strand at 141.656 kb on - strand at 141.656 kb on - strand at 141.682 kb on - strand at 141.692 kb on - strand at 141.692 kb on - strand at 141.703 kb on - strand at 141.710 kb on + strand at 141.710 kb on + strand at 141.712 kb on + strand at 141.718 kb on + strand, within CCNA_00132 at 141.718 kb on - strand, within CCNA_00132 at 141.718 kb on - strand, within CCNA_00132 at 141.722 kb on + strand, within CCNA_00132 at 141.787 kb on - strand, within CCNA_00132 at 141.802 kb on - strand, within CCNA_00132 at 141.816 kb on - strand, within CCNA_00132 at 141.832 kb on + strand, within CCNA_00132 at 141.868 kb on + strand, within CCNA_00132 at 141.874 kb on - strand, within CCNA_00132 at 141.882 kb on + strand, within CCNA_00132 at 141.901 kb on + strand, within CCNA_00132 at 141.911 kb on + strand, within CCNA_00132 at 141.919 kb on - strand, within CCNA_00132 at 141.927 kb on + strand, within CCNA_00132 at 141.927 kb on - strand, within CCNA_00132 at 141.934 kb on + strand, within CCNA_00132 at 141.955 kb on - strand, within CCNA_00132 at 142.015 kb on - strand, within CCNA_00132 at 142.032 kb on - strand, within CCNA_00132 at 142.032 kb on - strand, within CCNA_00132 at 142.067 kb on - strand, within CCNA_00132 at 142.068 kb on - strand, within CCNA_00132 at 142.081 kb on + strand, within CCNA_00132 at 142.093 kb on - strand, within CCNA_00132 at 142.109 kb on - strand, within CCNA_00132 at 142.113 kb on + strand, within CCNA_00132 at 142.123 kb on - strand, within CCNA_00132 at 142.134 kb on - strand, within CCNA_00132 at 142.139 kb on - strand, within CCNA_00132 at 142.140 kb on - strand, within CCNA_00132 at 142.175 kb on + strand, within CCNA_00132 at 142.175 kb on + strand, within CCNA_00132 at 142.176 kb on - strand, within CCNA_00132 at 142.193 kb on - strand, within CCNA_00132 at 142.211 kb on + strand, within CCNA_00132 at 142.251 kb on + strand, within CCNA_00132 at 142.322 kb on - strand, within CCNA_00132 at 142.340 kb on + strand, within CCNA_00132 at 142.348 kb on - strand, within CCNA_00132 at 142.361 kb on + strand at 142.370 kb on - strand at 142.381 kb on + strand at 142.386 kb on + strand at 142.394 kb on - strand at 142.428 kb on + strand at 142.428 kb on + strand at 142.428 kb on + strand at 142.428 kb on + strand at 142.436 kb on - strand at 142.436 kb on - strand at 142.455 kb on + strand at 142.502 kb on - strand at 142.507 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with CHIR-090 40 ug/mL remove 140,903 - -0.3 140,925 - -0.2 140,925 - +0.8 140,933 - +0.4 140,933 - -0.1 140,952 - +0.8 140,989 - -0.1 141,022 + -0.7 141,022 + -0.4 141,035 - -0.8 141,048 + -0.2 141,072 + +0.3 141,099 + +0.1 141,155 + -0.5 141,160 + +0.0 141,160 + -0.2 141,163 - CCNA_00131 0.10 -2.0 141,184 + CCNA_00131 0.15 -0.1 141,213 - CCNA_00131 0.22 -3.3 141,218 - CCNA_00131 0.23 -1.3 141,233 - CCNA_00131 0.27 -1.4 141,233 - CCNA_00131 0.27 +1.4 141,243 - CCNA_00131 0.29 -2.4 141,259 + CCNA_00131 0.33 +0.7 141,264 + CCNA_00131 0.34 +0.2 141,274 + CCNA_00131 0.37 -0.8 141,274 + CCNA_00131 0.37 +0.2 141,274 + CCNA_00131 0.37 -2.8 141,282 - CCNA_00131 0.39 -1.3 141,282 - CCNA_00131 0.39 -2.6 141,292 - CCNA_00131 0.41 -0.9 141,307 + CCNA_00131 0.44 +0.7 141,328 + CCNA_00131 0.49 +0.9 141,328 + CCNA_00131 0.49 -0.4 141,328 + CCNA_00131 0.49 +0.3 141,364 + CCNA_00131 0.58 +0.7 141,372 - CCNA_00131 0.60 -0.9 141,374 + CCNA_00131 0.60 -1.3 141,382 - CCNA_00131 0.62 -0.2 141,417 - CCNA_00131 0.70 -0.9 141,481 + CCNA_00131 0.86 +1.3 141,489 - CCNA_00131 0.87 +0.1 141,505 + +0.1 141,531 + -0.1 141,531 + -0.4 141,531 + +0.6 141,531 + +1.1 141,533 + +0.5 141,539 - -1.1 141,539 - -0.3 141,539 - +0.9 141,539 - -0.6 141,591 - +0.9 141,613 - +0.1 141,638 - +0.1 141,648 + +0.6 141,656 - +1.4 141,656 - +0.3 141,682 - -1.1 141,692 - -0.5 141,692 - -0.3 141,703 - +1.3 141,710 + -0.1 141,710 + +1.3 141,712 + -0.3 141,718 + CCNA_00132 0.11 +0.1 141,718 - CCNA_00132 0.11 +1.0 141,718 - CCNA_00132 0.11 +0.4 141,722 + CCNA_00132 0.11 +0.0 141,787 - CCNA_00132 0.19 -0.3 141,802 - CCNA_00132 0.21 -0.3 141,816 - CCNA_00132 0.23 +0.2 141,832 + CCNA_00132 0.25 -0.3 141,868 + CCNA_00132 0.29 -0.1 141,874 - CCNA_00132 0.30 -0.3 141,882 + CCNA_00132 0.31 +0.3 141,901 + CCNA_00132 0.33 -0.3 141,911 + CCNA_00132 0.35 -0.2 141,919 - CCNA_00132 0.36 -0.0 141,927 + CCNA_00132 0.37 +0.6 141,927 - CCNA_00132 0.37 +0.1 141,934 + CCNA_00132 0.38 -0.4 141,955 - CCNA_00132 0.40 +0.1 142,015 - CCNA_00132 0.48 +0.1 142,032 - CCNA_00132 0.50 -0.8 142,032 - CCNA_00132 0.50 +0.8 142,067 - CCNA_00132 0.54 -1.0 142,068 - CCNA_00132 0.54 -0.4 142,081 + CCNA_00132 0.56 +0.5 142,093 - CCNA_00132 0.57 -0.2 142,109 - CCNA_00132 0.59 +0.6 142,113 + CCNA_00132 0.60 +0.6 142,123 - CCNA_00132 0.61 -1.5 142,134 - CCNA_00132 0.63 +0.1 142,139 - CCNA_00132 0.63 -0.9 142,140 - CCNA_00132 0.63 -0.4 142,175 + CCNA_00132 0.68 +1.3 142,175 + CCNA_00132 0.68 +0.6 142,176 - CCNA_00132 0.68 +0.2 142,193 - CCNA_00132 0.70 -0.4 142,211 + CCNA_00132 0.72 -0.1 142,251 + CCNA_00132 0.77 -0.5 142,322 - CCNA_00132 0.86 +0.2 142,340 + CCNA_00132 0.88 +0.6 142,348 - CCNA_00132 0.89 +0.2 142,361 + -0.7 142,370 - -0.5 142,381 + -0.3 142,386 + +0.1 142,394 - -0.2 142,428 + +2.1 142,428 + +1.1 142,428 + +0.5 142,428 + +0.3 142,436 - -0.2 142,436 - +0.3 142,455 + +0.2 142,502 - -0.3 142,507 + +0.1
Or see this region's nucleotide sequence