Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS22925 and AMB_RS22930 are separated by 3 nucleotides AMB_RS22930 and AMB_RS22935 are separated by 1 nucleotides AMB_RS22935 and AMB_RS22940 are separated by 31 nucleotides AMB_RS22940 and AMB_RS23675 are separated by 178 nucleotides
AMB_RS22925: AMB_RS22925 - imidazole glycerol phosphate synthase cyclase subunit, at 4,942,751 to 4,943,509
_RS22925
AMB_RS22930: AMB_RS22930 - 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase, at 4,943,513 to 4,944,232
_RS22930
AMB_RS22935: AMB_RS22935 - imidazole glycerol phosphate synthase subunit HisH, at 4,944,234 to 4,944,884
_RS22935
AMB_RS22940: AMB_RS22940 - imidazoleglycerol-phosphate dehydratase, at 4,944,916 to 4,945,515
_RS22940
AMB_RS23675: AMB_RS23675 - Zn-dependent protease with chaperone function, at 4,945,694 to 4,946,815
_RS23675
Position (kb) 4944
4945 Strain fitness (log2 ratio) -1
0
1 at 4945.606 kb on + strand at 4945.647 kb on + strand at 4945.647 kb on + strand at 4945.814 kb on + strand, within AMB_RS23675 at 4945.814 kb on + strand, within AMB_RS23675 at 4945.815 kb on - strand, within AMB_RS23675 at 4945.815 kb on - strand, within AMB_RS23675 at 4945.815 kb on - strand, within AMB_RS23675
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 4,945,606 + -0.2 4,945,647 + +0.4 4,945,647 + -0.9 4,945,814 + AMB_RS23675 0.11 -0.8 4,945,814 + AMB_RS23675 0.11 -0.7 4,945,815 - AMB_RS23675 0.11 -0.5 4,945,815 - AMB_RS23675 0.11 -0.2 4,945,815 - AMB_RS23675 0.11 +0.4
Or see this region's nucleotide sequence