Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS17595 and AMB_RS17600 overlap by 8 nucleotides AMB_RS17600 and AMB_RS17605 overlap by 28 nucleotides AMB_RS17605 and AMB_RS17610 are separated by 137 nucleotides
AMB_RS17595: AMB_RS17595 - carboxymuconolactone decarboxylase family protein, at 3,822,315 to 3,822,755
_RS17595
AMB_RS17600: AMB_RS17600 - DNA-directed RNA polymerase specialized sigma subunit, at 3,822,748 to 3,823,614
_RS17600
AMB_RS17605: AMB_RS17605 - phosphoserine phosphatase SerB, at 3,823,587 to 3,824,483
_RS17605
AMB_RS17610: AMB_RS17610 - DUF1015 domain-containing protein, at 3,824,621 to 3,825,868
_RS17610
Position (kb)
3823
3824
3825 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3822.666 kb on + strand, within AMB_RS17595 at 3822.667 kb on - strand, within AMB_RS17595 at 3822.669 kb on - strand, within AMB_RS17595 at 3822.732 kb on + strand at 3822.732 kb on + strand at 3822.818 kb on - strand at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.099 kb on + strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.100 kb on - strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.124 kb on + strand, within AMB_RS17600 at 3823.125 kb on - strand, within AMB_RS17600 at 3823.292 kb on - strand, within AMB_RS17600 at 3823.555 kb on + strand at 3823.556 kb on - strand at 3823.589 kb on - strand at 3823.589 kb on - strand at 3823.612 kb on + strand at 3823.624 kb on + strand at 3824.522 kb on + strand at 3824.582 kb on + strand at 3824.583 kb on - strand at 3824.583 kb on - strand at 3824.699 kb on + strand at 3824.700 kb on - strand at 3824.808 kb on - strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.897 kb on + strand, within AMB_RS17610 at 3824.898 kb on - strand, within AMB_RS17610 at 3824.898 kb on - strand, within AMB_RS17610 at 3824.903 kb on + strand, within AMB_RS17610 at 3824.966 kb on + strand, within AMB_RS17610 at 3824.966 kb on + strand, within AMB_RS17610 at 3824.967 kb on - strand, within AMB_RS17610 at 3824.967 kb on - strand, within AMB_RS17610 at 3824.967 kb on - strand, within AMB_RS17610 at 3824.967 kb on - strand, within AMB_RS17610 at 3825.138 kb on - strand, within AMB_RS17610 at 3825.138 kb on - strand, within AMB_RS17610 at 3825.138 kb on - strand, within AMB_RS17610 at 3825.242 kb on + strand, within AMB_RS17610 at 3825.242 kb on + strand, within AMB_RS17610 at 3825.242 kb on + strand, within AMB_RS17610 at 3825.243 kb on - strand, within AMB_RS17610 at 3825.243 kb on - strand, within AMB_RS17610 at 3825.243 kb on - strand, within AMB_RS17610 at 3825.335 kb on + strand, within AMB_RS17610 at 3825.335 kb on + strand, within AMB_RS17610 at 3825.336 kb on - strand, within AMB_RS17610 at 3825.389 kb on + strand, within AMB_RS17610 at 3825.389 kb on + strand, within AMB_RS17610
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 3,822,666 + AMB_RS17595 0.80 +0.3 3,822,667 - AMB_RS17595 0.80 +0.1 3,822,669 - AMB_RS17595 0.80 +0.3 3,822,732 + -0.4 3,822,732 + +0.2 3,822,818 - -0.4 3,823,099 + AMB_RS17600 0.40 -1.3 3,823,099 + AMB_RS17600 0.40 -0.1 3,823,099 + AMB_RS17600 0.40 -0.8 3,823,099 + AMB_RS17600 0.40 -1.0 3,823,099 + AMB_RS17600 0.40 -0.0 3,823,100 - AMB_RS17600 0.41 +1.3 3,823,100 - AMB_RS17600 0.41 -0.6 3,823,100 - AMB_RS17600 0.41 -0.4 3,823,124 + AMB_RS17600 0.43 -0.3 3,823,124 + AMB_RS17600 0.43 -0.0 3,823,124 + AMB_RS17600 0.43 -0.7 3,823,125 - AMB_RS17600 0.43 -0.4 3,823,292 - AMB_RS17600 0.63 +0.3 3,823,555 + -1.7 3,823,556 - -0.2 3,823,589 - -0.1 3,823,589 - -1.4 3,823,612 + -0.3 3,823,624 + -1.1 3,824,522 + -1.0 3,824,582 + +0.4 3,824,583 - +0.9 3,824,583 - -0.7 3,824,699 + +0.3 3,824,700 - -0.8 3,824,808 - AMB_RS17610 0.15 -0.5 3,824,897 + AMB_RS17610 0.22 -1.5 3,824,897 + AMB_RS17610 0.22 -0.6 3,824,897 + AMB_RS17610 0.22 +0.2 3,824,897 + AMB_RS17610 0.22 +0.2 3,824,897 + AMB_RS17610 0.22 -0.6 3,824,897 + AMB_RS17610 0.22 +1.3 3,824,897 + AMB_RS17610 0.22 -1.0 3,824,897 + AMB_RS17610 0.22 -0.1 3,824,897 + AMB_RS17610 0.22 -1.9 3,824,898 - AMB_RS17610 0.22 -0.5 3,824,898 - AMB_RS17610 0.22 +0.6 3,824,903 + AMB_RS17610 0.23 -1.7 3,824,966 + AMB_RS17610 0.28 -0.2 3,824,966 + AMB_RS17610 0.28 -0.2 3,824,967 - AMB_RS17610 0.28 -0.6 3,824,967 - AMB_RS17610 0.28 -0.2 3,824,967 - AMB_RS17610 0.28 -1.3 3,824,967 - AMB_RS17610 0.28 +0.5 3,825,138 - AMB_RS17610 0.41 -0.7 3,825,138 - AMB_RS17610 0.41 -2.0 3,825,138 - AMB_RS17610 0.41 -0.2 3,825,242 + AMB_RS17610 0.50 -0.7 3,825,242 + AMB_RS17610 0.50 -0.9 3,825,242 + AMB_RS17610 0.50 -0.2 3,825,243 - AMB_RS17610 0.50 +0.9 3,825,243 - AMB_RS17610 0.50 -0.2 3,825,243 - AMB_RS17610 0.50 +0.1 3,825,335 + AMB_RS17610 0.57 -0.4 3,825,335 + AMB_RS17610 0.57 -0.2 3,825,336 - AMB_RS17610 0.57 -3.2 3,825,389 + AMB_RS17610 0.62 -0.5 3,825,389 + AMB_RS17610 0.62 -0.0
Or see this region's nucleotide sequence