Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13440 and AMB_RS13445 are separated by 14 nucleotides AMB_RS13445 and AMB_RS13450 are separated by 2 nucleotides AMB_RS13450 and AMB_RS13455 are separated by 13 nucleotides
AMB_RS13440: AMB_RS13440 - formate dehydrogenase, at 2,861,816 to 2,864,701
_RS13440
AMB_RS13445: AMB_RS13445 - 4Fe-4S dicluster domain-containing protein, at 2,864,716 to 2,865,351
_RS13445
AMB_RS13450: AMB_RS13450 - hypothetical protein, at 2,865,354 to 2,865,617
_RS13450
AMB_RS13455: AMB_RS13455 - formate dehydrogenase subunit gamma, at 2,865,631 to 2,866,611
_RS13455
Position (kb)
2865
2866 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2864.435 kb on + strand at 2864.570 kb on + strand at 2864.627 kb on + strand at 2864.627 kb on + strand at 2864.628 kb on - strand at 2864.633 kb on + strand at 2864.633 kb on + strand at 2864.633 kb on + strand at 2864.699 kb on + strand at 2864.699 kb on + strand at 2864.700 kb on - strand at 2864.932 kb on + strand, within AMB_RS13445 at 2864.932 kb on + strand, within AMB_RS13445 at 2864.933 kb on - strand, within AMB_RS13445 at 2864.933 kb on - strand, within AMB_RS13445 at 2864.990 kb on - strand, within AMB_RS13445 at 2864.998 kb on + strand, within AMB_RS13445 at 2864.998 kb on + strand, within AMB_RS13445 at 2864.998 kb on + strand, within AMB_RS13445 at 2865.128 kb on - strand, within AMB_RS13445 at 2865.229 kb on + strand, within AMB_RS13445 at 2865.230 kb on - strand, within AMB_RS13445 at 2865.230 kb on - strand, within AMB_RS13445 at 2865.289 kb on + strand at 2865.289 kb on + strand at 2865.289 kb on + strand at 2865.289 kb on + strand at 2865.290 kb on - strand at 2865.349 kb on + strand at 2865.349 kb on + strand at 2865.349 kb on + strand at 2865.350 kb on - strand at 2865.350 kb on - strand at 2865.456 kb on + strand, within AMB_RS13450 at 2865.456 kb on + strand, within AMB_RS13450 at 2865.457 kb on - strand, within AMB_RS13450 at 2865.457 kb on - strand, within AMB_RS13450 at 2865.457 kb on - strand, within AMB_RS13450 at 2865.457 kb on - strand, within AMB_RS13450 at 2865.554 kb on + strand, within AMB_RS13450 at 2865.615 kb on + strand at 2865.793 kb on + strand, within AMB_RS13455 at 2865.794 kb on - strand, within AMB_RS13455 at 2865.884 kb on - strand, within AMB_RS13455 at 2865.884 kb on - strand, within AMB_RS13455 at 2865.932 kb on - strand, within AMB_RS13455 at 2865.932 kb on - strand, within AMB_RS13455 at 2865.993 kb on + strand, within AMB_RS13455 at 2865.994 kb on - strand, within AMB_RS13455 at 2866.192 kb on + strand, within AMB_RS13455 at 2866.195 kb on + strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.196 kb on - strand, within AMB_RS13455 at 2866.213 kb on - strand, within AMB_RS13455 at 2866.477 kb on + strand, within AMB_RS13455 at 2866.477 kb on + strand, within AMB_RS13455 at 2866.477 kb on + strand, within AMB_RS13455 at 2866.477 kb on + strand, within AMB_RS13455 at 2866.478 kb on - strand, within AMB_RS13455 at 2866.478 kb on - strand, within AMB_RS13455 at 2866.478 kb on - strand, within AMB_RS13455 at 2866.478 kb on - strand, within AMB_RS13455 at 2866.478 kb on - strand, within AMB_RS13455 at 2866.528 kb on + strand at 2866.528 kb on + strand at 2866.609 kb on + strand at 2866.610 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 2,864,435 + +0.6 2,864,570 + -0.8 2,864,627 + -0.4 2,864,627 + -0.4 2,864,628 - -0.0 2,864,633 + -0.4 2,864,633 + -1.4 2,864,633 + -2.6 2,864,699 + +1.3 2,864,699 + +0.4 2,864,700 - -0.0 2,864,932 + AMB_RS13445 0.34 -1.2 2,864,932 + AMB_RS13445 0.34 -0.9 2,864,933 - AMB_RS13445 0.34 +1.3 2,864,933 - AMB_RS13445 0.34 -0.6 2,864,990 - AMB_RS13445 0.43 -0.3 2,864,998 + AMB_RS13445 0.44 +0.0 2,864,998 + AMB_RS13445 0.44 -0.2 2,864,998 + AMB_RS13445 0.44 -0.6 2,865,128 - AMB_RS13445 0.65 -3.3 2,865,229 + AMB_RS13445 0.81 -0.5 2,865,230 - AMB_RS13445 0.81 -0.5 2,865,230 - AMB_RS13445 0.81 -0.7 2,865,289 + +0.1 2,865,289 + +0.1 2,865,289 + -0.9 2,865,289 + -0.0 2,865,290 - +1.6 2,865,349 + -0.6 2,865,349 + +0.2 2,865,349 + +0.2 2,865,350 - -3.2 2,865,350 - -1.4 2,865,456 + AMB_RS13450 0.39 +0.2 2,865,456 + AMB_RS13450 0.39 +0.3 2,865,457 - AMB_RS13450 0.39 -0.1 2,865,457 - AMB_RS13450 0.39 -1.2 2,865,457 - AMB_RS13450 0.39 -2.4 2,865,457 - AMB_RS13450 0.39 +0.4 2,865,554 + AMB_RS13450 0.76 -0.5 2,865,615 + -1.0 2,865,793 + AMB_RS13455 0.17 -1.6 2,865,794 - AMB_RS13455 0.17 +0.0 2,865,884 - AMB_RS13455 0.26 -1.4 2,865,884 - AMB_RS13455 0.26 -2.4 2,865,932 - AMB_RS13455 0.31 +0.0 2,865,932 - AMB_RS13455 0.31 -1.1 2,865,993 + AMB_RS13455 0.37 -0.3 2,865,994 - AMB_RS13455 0.37 -0.1 2,866,192 + AMB_RS13455 0.57 +0.1 2,866,195 + AMB_RS13455 0.57 -0.1 2,866,196 - AMB_RS13455 0.58 -0.2 2,866,196 - AMB_RS13455 0.58 -0.1 2,866,196 - AMB_RS13455 0.58 -0.2 2,866,196 - AMB_RS13455 0.58 -1.9 2,866,196 - AMB_RS13455 0.58 -0.4 2,866,196 - AMB_RS13455 0.58 -0.2 2,866,213 - AMB_RS13455 0.59 -0.0 2,866,477 + AMB_RS13455 0.86 +1.5 2,866,477 + AMB_RS13455 0.86 -0.2 2,866,477 + AMB_RS13455 0.86 -0.4 2,866,477 + AMB_RS13455 0.86 -0.4 2,866,478 - AMB_RS13455 0.86 +1.4 2,866,478 - AMB_RS13455 0.86 -0.2 2,866,478 - AMB_RS13455 0.86 -0.4 2,866,478 - AMB_RS13455 0.86 -0.5 2,866,478 - AMB_RS13455 0.86 -0.5 2,866,528 + +0.2 2,866,528 + -0.2 2,866,609 + -2.1 2,866,610 - +0.1
Or see this region's nucleotide sequence