Strain Fitness in Magnetospirillum magneticum AMB-1 around AMB_RS11825

Experiment: 150_initial_1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAMB_RS11815 and AMB_RS11820 are separated by 69 nucleotidesAMB_RS11820 and AMB_RS11825 are separated by 31 nucleotidesAMB_RS11825 and AMB_RS11830 are separated by 18 nucleotidesAMB_RS11830 and AMB_RS11835 overlap by 29 nucleotides AMB_RS11815: AMB_RS11815 - serine hydroxymethyltransferase, at 2,545,611 to 2,546,894 _RS11815 AMB_RS11820: AMB_RS11820 - transcriptional regulator NrdR, at 2,546,964 to 2,547,425 _RS11820 AMB_RS11825: AMB_RS11825 - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,547,457 to 2,548,533 _RS11825 AMB_RS11830: AMB_RS11830 - riboflavin synthase, at 2,548,552 to 2,549,144 _RS11830 AMB_RS11835: AMB_RS11835 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 2,549,116 to 2,550,264 _RS11835 Position (kb) 2547 2548 2549Strain fitness (log2 ratio) -1 0 1at 2546.886 kb on + strandat 2546.887 kb on - strandat 2546.887 kb on - strandat 2546.892 kb on + strandat 2546.893 kb on - strandat 2547.026 kb on + strand, within AMB_RS11820at 2547.185 kb on - strand, within AMB_RS11820at 2547.333 kb on + strand, within AMB_RS11820at 2547.333 kb on + strand, within AMB_RS11820at 2548.529 kb on + strandat 2548.531 kb on + strandat 2548.531 kb on + strandat 2549.170 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 150_initial_1
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2,546,886 + -0.2
2,546,887 - -0.6
2,546,887 - +1.4
2,546,892 + -0.3
2,546,893 - -0.1
2,547,026 + AMB_RS11820 0.13 -1.0
2,547,185 - AMB_RS11820 0.48 -1.3
2,547,333 + AMB_RS11820 0.80 -0.1
2,547,333 + AMB_RS11820 0.80 +0.1
2,548,529 + +0.0
2,548,531 + +0.3
2,548,531 + +0.4
2,549,170 + +0.0

Or see this region's nucleotide sequence