Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS11190 and AMB_RS11195 are separated by 27 nucleotides AMB_RS11195 and AMB_RS11200 are separated by 18 nucleotides AMB_RS11200 and AMB_RS11205 are separated by 53 nucleotides
AMB_RS11190: AMB_RS11190 - phosphoadenylyl-sulfate reductase, at 2,408,660 to 2,409,358
_RS11190
AMB_RS11195: AMB_RS11195 - sulfate adenylyltransferase subunit 2, at 2,409,386 to 2,410,186
_RS11195
AMB_RS11200: AMB_RS11200 - adenylyl-sulfate kinase, at 2,410,205 to 2,412,130
_RS11200
AMB_RS11205: AMB_RS11205 - two-component sensor histidine kinase, at 2,412,184 to 2,414,055
_RS11205
Position (kb)
2410
2411
2412
2413 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.373 kb on + strand at 2409.374 kb on - strand at 2409.422 kb on + strand at 2409.953 kb on + strand, within AMB_RS11195 at 2409.989 kb on + strand, within AMB_RS11195 at 2410.228 kb on + strand at 2410.228 kb on + strand at 2411.966 kb on + strand at 2412.071 kb on + strand at 2412.185 kb on + strand at 2412.234 kb on + strand at 2412.235 kb on - strand at 2412.235 kb on - strand at 2412.276 kb on + strand at 2412.363 kb on - strand at 2412.363 kb on - strand at 2412.433 kb on - strand, within AMB_RS11205 at 2412.507 kb on + strand, within AMB_RS11205 at 2412.507 kb on + strand, within AMB_RS11205 at 2412.508 kb on - strand, within AMB_RS11205 at 2412.665 kb on + strand, within AMB_RS11205 at 2412.720 kb on - strand, within AMB_RS11205 at 2412.775 kb on + strand, within AMB_RS11205 at 2412.776 kb on - strand, within AMB_RS11205 at 2412.862 kb on - strand, within AMB_RS11205 at 2412.919 kb on - strand, within AMB_RS11205 at 2413.061 kb on - strand, within AMB_RS11205
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 2,409,373 + -0.5 2,409,373 + -1.3 2,409,373 + -0.5 2,409,373 + -2.3 2,409,374 - +0.4 2,409,422 + -0.7 2,409,953 + AMB_RS11195 0.71 -1.4 2,409,989 + AMB_RS11195 0.75 -2.8 2,410,228 + -0.1 2,410,228 + -0.4 2,411,966 + -1.1 2,412,071 + +0.4 2,412,185 + +0.2 2,412,234 + +2.5 2,412,235 - -1.2 2,412,235 - -2.4 2,412,276 + +0.7 2,412,363 - +0.4 2,412,363 - -0.6 2,412,433 - AMB_RS11205 0.13 +0.3 2,412,507 + AMB_RS11205 0.17 +0.2 2,412,507 + AMB_RS11205 0.17 -0.6 2,412,508 - AMB_RS11205 0.17 -2.2 2,412,665 + AMB_RS11205 0.26 +0.4 2,412,720 - AMB_RS11205 0.29 +0.4 2,412,775 + AMB_RS11205 0.32 +1.2 2,412,776 - AMB_RS11205 0.32 +1.0 2,412,862 - AMB_RS11205 0.36 -0.1 2,412,919 - AMB_RS11205 0.39 -0.6 2,413,061 - AMB_RS11205 0.47 -0.7
Or see this region's nucleotide sequence