Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10760 and AMB_RS10765 are separated by 152 nucleotides AMB_RS10765 and AMB_RS10770 are separated by 77 nucleotides AMB_RS10770 and AMB_RS10775 are separated by 90 nucleotides
AMB_RS10760: AMB_RS10760 - hypothetical protein, at 2,305,744 to 2,306,073
_RS10760
AMB_RS10765: AMB_RS10765 - septal ring lytic transglycosylase RlpA family protein, at 2,306,226 to 2,306,597
_RS10765
AMB_RS10770: AMB_RS10770 - hypothetical protein, at 2,306,675 to 2,307,139
_RS10770
AMB_RS10775: AMB_RS10775 - CoA transferase, at 2,307,230 to 2,308,438
_RS10775
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2305.842 kb on + strand, within AMB_RS10760 at 2305.843 kb on - strand, within AMB_RS10760 at 2305.843 kb on - strand, within AMB_RS10760 at 2305.843 kb on - strand, within AMB_RS10760 at 2305.843 kb on - strand, within AMB_RS10760 at 2305.861 kb on + strand, within AMB_RS10760 at 2306.093 kb on + strand at 2306.100 kb on - strand at 2306.307 kb on + strand, within AMB_RS10765 at 2306.307 kb on + strand, within AMB_RS10765 at 2306.307 kb on + strand, within AMB_RS10765 at 2306.308 kb on - strand, within AMB_RS10765 at 2306.308 kb on - strand, within AMB_RS10765 at 2306.399 kb on + strand, within AMB_RS10765 at 2306.400 kb on - strand, within AMB_RS10765 at 2306.400 kb on - strand, within AMB_RS10765 at 2306.431 kb on + strand, within AMB_RS10765 at 2306.487 kb on + strand, within AMB_RS10765 at 2306.487 kb on + strand, within AMB_RS10765 at 2306.488 kb on - strand, within AMB_RS10765 at 2306.488 kb on - strand, within AMB_RS10765 at 2306.547 kb on + strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.548 kb on - strand, within AMB_RS10765 at 2306.595 kb on + strand at 2306.660 kb on - strand at 2306.677 kb on - strand at 2306.677 kb on - strand at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.766 kb on + strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2306.767 kb on - strand, within AMB_RS10770 at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.250 kb on + strand at 2307.822 kb on + strand, within AMB_RS10775 at 2307.822 kb on + strand, within AMB_RS10775 at 2307.822 kb on + strand, within AMB_RS10775 at 2307.823 kb on - strand, within AMB_RS10775 at 2307.823 kb on - strand, within AMB_RS10775 at 2307.915 kb on + strand, within AMB_RS10775 at 2307.915 kb on + strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.916 kb on - strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.975 kb on + strand, within AMB_RS10775 at 2307.976 kb on - strand, within AMB_RS10775 at 2308.029 kb on + strand, within AMB_RS10775 at 2308.030 kb on - strand, within AMB_RS10775 at 2308.047 kb on + strand, within AMB_RS10775 at 2308.048 kb on - strand, within AMB_RS10775
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 2,305,842 + AMB_RS10760 0.30 +0.9 2,305,843 - AMB_RS10760 0.30 -0.2 2,305,843 - AMB_RS10760 0.30 +0.2 2,305,843 - AMB_RS10760 0.30 -0.2 2,305,843 - AMB_RS10760 0.30 -0.7 2,305,861 + AMB_RS10760 0.35 +0.6 2,306,093 + +1.0 2,306,100 - +0.1 2,306,307 + AMB_RS10765 0.22 -1.0 2,306,307 + AMB_RS10765 0.22 +0.2 2,306,307 + AMB_RS10765 0.22 -0.6 2,306,308 - AMB_RS10765 0.22 -0.6 2,306,308 - AMB_RS10765 0.22 -2.2 2,306,399 + AMB_RS10765 0.47 +0.2 2,306,400 - AMB_RS10765 0.47 +0.2 2,306,400 - AMB_RS10765 0.47 +1.2 2,306,431 + AMB_RS10765 0.55 +0.1 2,306,487 + AMB_RS10765 0.70 +0.6 2,306,487 + AMB_RS10765 0.70 -1.4 2,306,488 - AMB_RS10765 0.70 -0.2 2,306,488 - AMB_RS10765 0.70 +0.2 2,306,547 + AMB_RS10765 0.86 +0.3 2,306,548 - AMB_RS10765 0.87 -0.2 2,306,548 - AMB_RS10765 0.87 -0.6 2,306,548 - AMB_RS10765 0.87 +2.4 2,306,548 - AMB_RS10765 0.87 -0.1 2,306,595 + +0.7 2,306,660 - +0.5 2,306,677 - +0.4 2,306,677 - -0.7 2,306,766 + AMB_RS10770 0.20 -0.0 2,306,766 + AMB_RS10770 0.20 +0.4 2,306,766 + AMB_RS10770 0.20 -1.8 2,306,766 + AMB_RS10770 0.20 +0.2 2,306,767 - AMB_RS10770 0.20 -1.6 2,306,767 - AMB_RS10770 0.20 -0.2 2,306,767 - AMB_RS10770 0.20 +0.1 2,306,767 - AMB_RS10770 0.20 -1.5 2,307,250 + +1.4 2,307,250 + +1.4 2,307,250 + -0.7 2,307,250 + -1.0 2,307,822 + AMB_RS10775 0.49 -0.5 2,307,822 + AMB_RS10775 0.49 -0.3 2,307,822 + AMB_RS10775 0.49 +0.4 2,307,823 - AMB_RS10775 0.49 -0.3 2,307,823 - AMB_RS10775 0.49 -0.1 2,307,915 + AMB_RS10775 0.57 -0.4 2,307,915 + AMB_RS10775 0.57 -1.3 2,307,916 - AMB_RS10775 0.57 +0.3 2,307,916 - AMB_RS10775 0.57 +1.0 2,307,916 - AMB_RS10775 0.57 +0.2 2,307,916 - AMB_RS10775 0.57 +0.6 2,307,975 + AMB_RS10775 0.62 -0.5 2,307,975 + AMB_RS10775 0.62 +0.2 2,307,975 + AMB_RS10775 0.62 +0.2 2,307,976 - AMB_RS10775 0.62 -1.0 2,308,029 + AMB_RS10775 0.66 -1.1 2,308,030 - AMB_RS10775 0.66 -1.9 2,308,047 + AMB_RS10775 0.68 +2.0 2,308,048 - AMB_RS10775 0.68 +0.1
Or see this region's nucleotide sequence