Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10535 and AMB_RS10540 are separated by 184 nucleotides AMB_RS10540 and AMB_RS10545 overlap by 1 nucleotides
AMB_RS10535: AMB_RS10535 - hypothetical protein, at 2,262,739 to 2,264,064
_RS10535
AMB_RS10540: AMB_RS10540 - KR domain-containing protein, at 2,264,249 to 2,264,968
_RS10540
AMB_RS10545: AMB_RS10545 - amidophosphoribosyltransferase, at 2,264,968 to 2,266,431
_RS10545
Position (kb)
2264
2265 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2263.251 kb on + strand, within AMB_RS10535 at 2263.251 kb on + strand, within AMB_RS10535 at 2263.251 kb on + strand, within AMB_RS10535 at 2263.252 kb on - strand, within AMB_RS10535 at 2263.252 kb on - strand, within AMB_RS10535 at 2263.252 kb on - strand, within AMB_RS10535 at 2263.252 kb on - strand, within AMB_RS10535 at 2263.252 kb on - strand, within AMB_RS10535 at 2263.290 kb on + strand, within AMB_RS10535 at 2263.290 kb on + strand, within AMB_RS10535 at 2263.291 kb on - strand, within AMB_RS10535 at 2263.291 kb on - strand, within AMB_RS10535 at 2263.291 kb on - strand, within AMB_RS10535 at 2263.291 kb on - strand, within AMB_RS10535 at 2263.291 kb on - strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.292 kb on + strand, within AMB_RS10535 at 2263.293 kb on - strand, within AMB_RS10535 at 2263.293 kb on - strand, within AMB_RS10535 at 2263.293 kb on - strand, within AMB_RS10535 at 2263.293 kb on - strand, within AMB_RS10535 at 2263.293 kb on - strand, within AMB_RS10535 at 2263.460 kb on + strand, within AMB_RS10535 at 2264.005 kb on - strand at 2264.005 kb on - strand at 2264.005 kb on - strand at 2264.005 kb on - strand at 2264.081 kb on + strand at 2264.082 kb on - strand at 2264.108 kb on + strand at 2264.161 kb on + strand at 2264.244 kb on - strand at 2264.493 kb on + strand, within AMB_RS10540 at 2264.493 kb on + strand, within AMB_RS10540 at 2264.493 kb on + strand, within AMB_RS10540 at 2264.493 kb on + strand, within AMB_RS10540 at 2264.494 kb on - strand, within AMB_RS10540 at 2264.494 kb on - strand, within AMB_RS10540 at 2265.029 kb on + strand at 2265.029 kb on + strand at 2265.029 kb on + strand at 2265.029 kb on + strand at 2265.030 kb on - strand at 2265.030 kb on - strand at 2265.030 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 2,263,251 + AMB_RS10535 0.39 +0.1 2,263,251 + AMB_RS10535 0.39 -0.5 2,263,251 + AMB_RS10535 0.39 +0.1 2,263,252 - AMB_RS10535 0.39 +0.1 2,263,252 - AMB_RS10535 0.39 +0.2 2,263,252 - AMB_RS10535 0.39 +0.0 2,263,252 - AMB_RS10535 0.39 +0.2 2,263,252 - AMB_RS10535 0.39 -0.2 2,263,290 + AMB_RS10535 0.42 +0.1 2,263,290 + AMB_RS10535 0.42 -0.3 2,263,291 - AMB_RS10535 0.42 -0.3 2,263,291 - AMB_RS10535 0.42 +0.4 2,263,291 - AMB_RS10535 0.42 -0.9 2,263,291 - AMB_RS10535 0.42 -0.1 2,263,291 - AMB_RS10535 0.42 -1.0 2,263,292 + AMB_RS10535 0.42 +1.0 2,263,292 + AMB_RS10535 0.42 -0.7 2,263,292 + AMB_RS10535 0.42 -1.2 2,263,292 + AMB_RS10535 0.42 -0.1 2,263,292 + AMB_RS10535 0.42 +0.1 2,263,292 + AMB_RS10535 0.42 -0.5 2,263,292 + AMB_RS10535 0.42 -0.6 2,263,293 - AMB_RS10535 0.42 -0.8 2,263,293 - AMB_RS10535 0.42 +0.3 2,263,293 - AMB_RS10535 0.42 -0.5 2,263,293 - AMB_RS10535 0.42 +0.0 2,263,293 - AMB_RS10535 0.42 -0.2 2,263,460 + AMB_RS10535 0.54 -0.6 2,264,005 - -0.7 2,264,005 - +0.1 2,264,005 - +0.9 2,264,005 - -0.4 2,264,081 + -0.1 2,264,082 - -0.2 2,264,108 + +0.4 2,264,161 + +0.1 2,264,244 - +0.2 2,264,493 + AMB_RS10540 0.34 -0.5 2,264,493 + AMB_RS10540 0.34 -0.8 2,264,493 + AMB_RS10540 0.34 -0.4 2,264,493 + AMB_RS10540 0.34 -0.6 2,264,494 - AMB_RS10540 0.34 -0.5 2,264,494 - AMB_RS10540 0.34 -0.3 2,265,029 + +1.3 2,265,029 + +0.2 2,265,029 + -0.4 2,265,029 + -0.3 2,265,030 - -2.9 2,265,030 - -0.1 2,265,030 - -0.1
Or see this region's nucleotide sequence