Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS10115 and AMB_RS10120 are separated by 14 nucleotides AMB_RS10120 and AMB_RS10125 are separated by 126 nucleotides AMB_RS10125 and AMB_RS10130 are separated by 2 nucleotides AMB_RS10130 and AMB_RS10135 are separated by 4 nucleotides
AMB_RS10115: AMB_RS10115 - aminoacyl-tRNA hydrolase, at 2,179,513 to 2,179,932
_RS10115
AMB_RS10120: AMB_RS10120 - DUF1476 domain-containing protein, at 2,179,947 to 2,180,288
_RS10120
AMB_RS10125: AMB_RS10125 - sulfate ABC transporter substrate-binding protein, at 2,180,415 to 2,181,458
_RS10125
AMB_RS10130: AMB_RS10130 - sulfate ABC transporter permease subunit CysT, at 2,181,461 to 2,182,318
_RS10130
AMB_RS10135: AMB_RS10135 - sulfate ABC transporter permease subunit CysW, at 2,182,323 to 2,183,162
_RS10135
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2179.424 kb on + strand at 2179.424 kb on + strand at 2179.424 kb on + strand at 2179.424 kb on + strand at 2179.424 kb on + strand at 2179.425 kb on - strand at 2179.425 kb on - strand at 2179.440 kb on - strand at 2179.503 kb on - strand at 2179.504 kb on + strand at 2179.505 kb on - strand at 2179.505 kb on - strand at 2179.505 kb on - strand at 2179.505 kb on - strand at 2179.605 kb on + strand, within AMB_RS10115 at 2179.605 kb on + strand, within AMB_RS10115 at 2179.606 kb on - strand, within AMB_RS10115 at 2179.606 kb on - strand, within AMB_RS10115 at 2179.606 kb on - strand, within AMB_RS10115 at 2179.606 kb on - strand, within AMB_RS10115 at 2179.731 kb on + strand, within AMB_RS10115 at 2179.744 kb on + strand, within AMB_RS10115 at 2179.745 kb on - strand, within AMB_RS10115 at 2179.930 kb on + strand at 2179.930 kb on + strand at 2179.968 kb on + strand at 2179.968 kb on + strand at 2179.969 kb on - strand at 2179.969 kb on - strand at 2180.097 kb on + strand, within AMB_RS10120 at 2180.097 kb on + strand, within AMB_RS10120 at 2180.097 kb on + strand, within AMB_RS10120 at 2180.098 kb on - strand, within AMB_RS10120 at 2180.098 kb on - strand, within AMB_RS10120 at 2180.379 kb on - strand at 2180.387 kb on - strand at 2180.387 kb on - strand at 2180.550 kb on + strand, within AMB_RS10125 at 2180.550 kb on + strand, within AMB_RS10125 at 2180.550 kb on + strand, within AMB_RS10125 at 2180.550 kb on + strand, within AMB_RS10125 at 2180.550 kb on + strand, within AMB_RS10125 at 2180.550 kb on + strand, within AMB_RS10125 at 2180.551 kb on - strand, within AMB_RS10125 at 2180.551 kb on - strand, within AMB_RS10125 at 2180.551 kb on - strand, within AMB_RS10125 at 2180.551 kb on - strand, within AMB_RS10125 at 2180.551 kb on - strand, within AMB_RS10125 at 2180.571 kb on + strand, within AMB_RS10125 at 2180.572 kb on - strand, within AMB_RS10125 at 2180.572 kb on - strand, within AMB_RS10125 at 2180.721 kb on + strand, within AMB_RS10125 at 2180.721 kb on + strand, within AMB_RS10125 at 2180.722 kb on - strand, within AMB_RS10125 at 2180.722 kb on - strand, within AMB_RS10125 at 2180.787 kb on + strand, within AMB_RS10125 at 2180.787 kb on + strand, within AMB_RS10125 at 2180.787 kb on + strand, within AMB_RS10125 at 2180.788 kb on - strand, within AMB_RS10125 at 2180.788 kb on - strand, within AMB_RS10125 at 2180.788 kb on - strand, within AMB_RS10125 at 2180.789 kb on + strand, within AMB_RS10125 at 2180.790 kb on - strand, within AMB_RS10125 at 2180.790 kb on - strand, within AMB_RS10125 at 2180.802 kb on + strand, within AMB_RS10125 at 2180.803 kb on - strand, within AMB_RS10125 at 2180.803 kb on - strand, within AMB_RS10125 at 2180.906 kb on + strand, within AMB_RS10125 at 2180.906 kb on + strand, within AMB_RS10125 at 2180.906 kb on + strand, within AMB_RS10125 at 2180.907 kb on - strand, within AMB_RS10125 at 2180.907 kb on - strand, within AMB_RS10125 at 2180.934 kb on + strand, within AMB_RS10125 at 2180.934 kb on + strand, within AMB_RS10125 at 2180.934 kb on + strand, within AMB_RS10125 at 2180.934 kb on + strand, within AMB_RS10125 at 2180.934 kb on + strand, within AMB_RS10125 at 2180.935 kb on - strand, within AMB_RS10125 at 2180.952 kb on + strand, within AMB_RS10125 at 2181.246 kb on + strand, within AMB_RS10125 at 2181.247 kb on - strand, within AMB_RS10125 at 2181.262 kb on - strand, within AMB_RS10125 at 2181.262 kb on - strand, within AMB_RS10125 at 2181.262 kb on - strand, within AMB_RS10125 at 2181.262 kb on - strand, within AMB_RS10125 at 2181.300 kb on + strand, within AMB_RS10125 at 2181.300 kb on + strand, within AMB_RS10125 at 2181.300 kb on + strand, within AMB_RS10125 at 2181.300 kb on + strand, within AMB_RS10125 at 2181.554 kb on + strand, within AMB_RS10130 at 2181.554 kb on + strand, within AMB_RS10130 at 2181.554 kb on + strand, within AMB_RS10130 at 2181.555 kb on - strand, within AMB_RS10130 at 2181.555 kb on - strand, within AMB_RS10130 at 2181.555 kb on - strand, within AMB_RS10130 at 2181.843 kb on - strand, within AMB_RS10130 at 2182.122 kb on - strand, within AMB_RS10130 at 2182.205 kb on + strand, within AMB_RS10130 at 2182.206 kb on - strand, within AMB_RS10130 at 2182.211 kb on + strand, within AMB_RS10130 at 2182.211 kb on + strand, within AMB_RS10130 at 2182.212 kb on - strand, within AMB_RS10130 at 2182.212 kb on - strand, within AMB_RS10130 at 2182.212 kb on - strand, within AMB_RS10130 at 2182.212 kb on - strand, within AMB_RS10130 at 2182.212 kb on - strand, within AMB_RS10130
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 2,179,424 + +0.2 2,179,424 + -0.3 2,179,424 + +2.0 2,179,424 + +0.5 2,179,424 + -1.4 2,179,425 - -1.2 2,179,425 - -0.3 2,179,440 - -0.7 2,179,503 - +0.4 2,179,504 + +0.1 2,179,505 - +0.7 2,179,505 - +1.2 2,179,505 - -0.9 2,179,505 - -0.3 2,179,605 + AMB_RS10115 0.22 +1.2 2,179,605 + AMB_RS10115 0.22 -0.3 2,179,606 - AMB_RS10115 0.22 +0.6 2,179,606 - AMB_RS10115 0.22 -0.7 2,179,606 - AMB_RS10115 0.22 -0.5 2,179,606 - AMB_RS10115 0.22 -0.6 2,179,731 + AMB_RS10115 0.52 -0.6 2,179,744 + AMB_RS10115 0.55 -1.4 2,179,745 - AMB_RS10115 0.55 -0.2 2,179,930 + +1.1 2,179,930 + +0.5 2,179,968 + +0.0 2,179,968 + -0.5 2,179,969 - -0.2 2,179,969 - +0.0 2,180,097 + AMB_RS10120 0.44 -0.3 2,180,097 + AMB_RS10120 0.44 -0.8 2,180,097 + AMB_RS10120 0.44 -0.4 2,180,098 - AMB_RS10120 0.44 -1.4 2,180,098 - AMB_RS10120 0.44 +0.8 2,180,379 - -1.5 2,180,387 - +0.1 2,180,387 - -0.3 2,180,550 + AMB_RS10125 0.13 -0.4 2,180,550 + AMB_RS10125 0.13 +0.5 2,180,550 + AMB_RS10125 0.13 +0.2 2,180,550 + AMB_RS10125 0.13 +0.9 2,180,550 + AMB_RS10125 0.13 +0.4 2,180,550 + AMB_RS10125 0.13 -1.2 2,180,551 - AMB_RS10125 0.13 -0.6 2,180,551 - AMB_RS10125 0.13 -1.0 2,180,551 - AMB_RS10125 0.13 +1.4 2,180,551 - AMB_RS10125 0.13 -0.4 2,180,551 - AMB_RS10125 0.13 +0.4 2,180,571 + AMB_RS10125 0.15 +0.1 2,180,572 - AMB_RS10125 0.15 -0.1 2,180,572 - AMB_RS10125 0.15 -0.6 2,180,721 + AMB_RS10125 0.29 -0.4 2,180,721 + AMB_RS10125 0.29 -0.8 2,180,722 - AMB_RS10125 0.29 -0.2 2,180,722 - AMB_RS10125 0.29 -1.8 2,180,787 + AMB_RS10125 0.36 -0.8 2,180,787 + AMB_RS10125 0.36 -0.3 2,180,787 + AMB_RS10125 0.36 +0.1 2,180,788 - AMB_RS10125 0.36 +0.7 2,180,788 - AMB_RS10125 0.36 -0.7 2,180,788 - AMB_RS10125 0.36 -0.4 2,180,789 + AMB_RS10125 0.36 -0.9 2,180,790 - AMB_RS10125 0.36 -0.2 2,180,790 - AMB_RS10125 0.36 -0.2 2,180,802 + AMB_RS10125 0.37 -0.7 2,180,803 - AMB_RS10125 0.37 -0.4 2,180,803 - AMB_RS10125 0.37 +0.1 2,180,906 + AMB_RS10125 0.47 -0.5 2,180,906 + AMB_RS10125 0.47 +1.6 2,180,906 + AMB_RS10125 0.47 -0.8 2,180,907 - AMB_RS10125 0.47 -0.8 2,180,907 - AMB_RS10125 0.47 -0.7 2,180,934 + AMB_RS10125 0.50 +0.2 2,180,934 + AMB_RS10125 0.50 -0.6 2,180,934 + AMB_RS10125 0.50 +0.3 2,180,934 + AMB_RS10125 0.50 +1.2 2,180,934 + AMB_RS10125 0.50 -0.3 2,180,935 - AMB_RS10125 0.50 -1.6 2,180,952 + AMB_RS10125 0.51 -0.8 2,181,246 + AMB_RS10125 0.80 -0.1 2,181,247 - AMB_RS10125 0.80 +0.4 2,181,262 - AMB_RS10125 0.81 +0.0 2,181,262 - AMB_RS10125 0.81 -0.3 2,181,262 - AMB_RS10125 0.81 +0.4 2,181,262 - AMB_RS10125 0.81 -0.3 2,181,300 + AMB_RS10125 0.85 -1.0 2,181,300 + AMB_RS10125 0.85 +0.1 2,181,300 + AMB_RS10125 0.85 -0.3 2,181,300 + AMB_RS10125 0.85 -0.3 2,181,554 + AMB_RS10130 0.11 +1.2 2,181,554 + AMB_RS10130 0.11 +1.1 2,181,554 + AMB_RS10130 0.11 +1.1 2,181,555 - AMB_RS10130 0.11 -0.1 2,181,555 - AMB_RS10130 0.11 +0.1 2,181,555 - AMB_RS10130 0.11 +0.5 2,181,843 - AMB_RS10130 0.45 -0.7 2,182,122 - AMB_RS10130 0.77 -0.1 2,182,205 + AMB_RS10130 0.87 +1.1 2,182,206 - AMB_RS10130 0.87 +0.0 2,182,211 + AMB_RS10130 0.87 +3.0 2,182,211 + AMB_RS10130 0.87 -3.0 2,182,212 - AMB_RS10130 0.88 -0.2 2,182,212 - AMB_RS10130 0.88 +0.1 2,182,212 - AMB_RS10130 0.88 +0.4 2,182,212 - AMB_RS10130 0.88 -1.3 2,182,212 - AMB_RS10130 0.88 -0.2
Or see this region's nucleotide sequence