Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05455 and AMB_RS05460 overlap by 49 nucleotides AMB_RS05460 and AMB_RS05465 overlap by 80 nucleotides AMB_RS05465 and AMB_RS05470 are separated by 67 nucleotides AMB_RS05470 and AMB_RS05475 overlap by 1 nucleotides
AMB_RS05455: AMB_RS05455 - glycosyltransferase family 2 protein, at 1,130,427 to 1,131,452
_RS05455
AMB_RS05460: AMB_RS05460 - UDP-N-acetylmuramyl pentapeptide phosphotransferase, at 1,131,404 to 1,132,267
_RS05460
AMB_RS05465: AMB_RS05465 - nucleoside-diphosphate sugar epimerase, at 1,132,188 to 1,133,195
_RS05465
AMB_RS05470: AMB_RS05470 - polysaccharide ABC transporter, at 1,133,263 to 1,133,997
_RS05470
AMB_RS05475: AMB_RS05475 - hypothetical protein, at 1,133,997 to 1,134,848
_RS05475
Position (kb)
1132
1133
1134 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1131.197 kb on - strand, within AMB_RS05455 at 1131.198 kb on + strand, within AMB_RS05455 at 1131.199 kb on - strand, within AMB_RS05455 at 1131.204 kb on + strand, within AMB_RS05455 at 1131.205 kb on - strand, within AMB_RS05455 at 1131.231 kb on - strand, within AMB_RS05455 at 1131.267 kb on + strand, within AMB_RS05455 at 1131.271 kb on - strand, within AMB_RS05455 at 1131.278 kb on + strand, within AMB_RS05455 at 1131.383 kb on + strand at 1131.383 kb on + strand at 1131.384 kb on - strand at 1131.406 kb on - strand at 1131.450 kb on + strand at 1131.521 kb on + strand, within AMB_RS05460 at 1131.637 kb on + strand, within AMB_RS05460 at 1131.638 kb on - strand, within AMB_RS05460 at 1132.189 kb on + strand at 1132.189 kb on + strand at 1132.190 kb on - strand at 1132.304 kb on + strand, within AMB_RS05465 at 1132.305 kb on - strand, within AMB_RS05465 at 1132.344 kb on - strand, within AMB_RS05465 at 1132.347 kb on + strand, within AMB_RS05465 at 1132.347 kb on + strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.348 kb on - strand, within AMB_RS05465 at 1132.385 kb on + strand, within AMB_RS05465 at 1132.719 kb on + strand, within AMB_RS05465 at 1132.741 kb on + strand, within AMB_RS05465 at 1132.837 kb on - strand, within AMB_RS05465 at 1132.859 kb on + strand, within AMB_RS05465 at 1132.859 kb on + strand, within AMB_RS05465 at 1132.859 kb on + strand, within AMB_RS05465 at 1132.859 kb on + strand, within AMB_RS05465 at 1132.859 kb on + strand, within AMB_RS05465 at 1132.860 kb on - strand, within AMB_RS05465 at 1132.860 kb on - strand, within AMB_RS05465 at 1132.896 kb on - strand, within AMB_RS05465 at 1132.960 kb on + strand, within AMB_RS05465 at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.247 kb on + strand at 1133.247 kb on + strand at 1133.247 kb on + strand at 1133.248 kb on - strand at 1133.248 kb on - strand at 1133.248 kb on - strand at 1133.248 kb on - strand at 1133.248 kb on - strand at 1133.456 kb on - strand, within AMB_RS05470 at 1133.524 kb on + strand, within AMB_RS05470 at 1133.525 kb on - strand, within AMB_RS05470 at 1133.791 kb on + strand, within AMB_RS05470 at 1133.792 kb on - strand, within AMB_RS05470 at 1133.948 kb on + strand at 1133.948 kb on + strand at 1133.949 kb on - strand at 1133.957 kb on + strand at 1133.957 kb on + strand at 1133.958 kb on - strand at 1133.958 kb on - strand at 1134.007 kb on + strand at 1134.007 kb on + strand at 1134.034 kb on + strand at 1134.036 kb on + strand at 1134.036 kb on + strand at 1134.037 kb on - strand at 1134.051 kb on + strand at 1134.173 kb on - strand, within AMB_RS05475
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 1,131,197 - AMB_RS05455 0.75 -0.4 1,131,198 + AMB_RS05455 0.75 -0.5 1,131,199 - AMB_RS05455 0.75 +0.5 1,131,204 + AMB_RS05455 0.76 -0.4 1,131,205 - AMB_RS05455 0.76 -0.8 1,131,231 - AMB_RS05455 0.78 +0.4 1,131,267 + AMB_RS05455 0.82 -0.1 1,131,271 - AMB_RS05455 0.82 -0.3 1,131,278 + AMB_RS05455 0.83 -1.1 1,131,383 + -0.1 1,131,383 + -0.1 1,131,384 - -0.1 1,131,406 - +0.6 1,131,450 + +0.1 1,131,521 + AMB_RS05460 0.14 +1.3 1,131,637 + AMB_RS05460 0.27 +1.4 1,131,638 - AMB_RS05460 0.27 -0.0 1,132,189 + +0.5 1,132,189 + -0.1 1,132,190 - -0.2 1,132,304 + AMB_RS05465 0.12 +0.1 1,132,305 - AMB_RS05465 0.12 +2.7 1,132,344 - AMB_RS05465 0.15 +0.1 1,132,347 + AMB_RS05465 0.16 +0.4 1,132,347 + AMB_RS05465 0.16 -0.0 1,132,348 - AMB_RS05465 0.16 -0.1 1,132,348 - AMB_RS05465 0.16 -0.5 1,132,348 - AMB_RS05465 0.16 +1.1 1,132,348 - AMB_RS05465 0.16 -0.3 1,132,385 + AMB_RS05465 0.20 -0.6 1,132,719 + AMB_RS05465 0.53 -0.3 1,132,741 + AMB_RS05465 0.55 +0.9 1,132,837 - AMB_RS05465 0.64 +0.4 1,132,859 + AMB_RS05465 0.67 +0.1 1,132,859 + AMB_RS05465 0.67 -0.0 1,132,859 + AMB_RS05465 0.67 +0.1 1,132,859 + AMB_RS05465 0.67 -0.1 1,132,859 + AMB_RS05465 0.67 -0.2 1,132,860 - AMB_RS05465 0.67 +0.2 1,132,860 - AMB_RS05465 0.67 -2.0 1,132,896 - AMB_RS05465 0.70 +0.4 1,132,960 + AMB_RS05465 0.77 -0.3 1,133,238 + -0.0 1,133,238 + -0.3 1,133,239 - +0.7 1,133,239 - -0.5 1,133,239 - +0.4 1,133,247 + -0.0 1,133,247 + -0.5 1,133,247 + +1.4 1,133,248 - -0.4 1,133,248 - -0.2 1,133,248 - -0.6 1,133,248 - -0.5 1,133,248 - +0.1 1,133,456 - AMB_RS05470 0.26 +1.1 1,133,524 + AMB_RS05470 0.36 -1.1 1,133,525 - AMB_RS05470 0.36 -0.2 1,133,791 + AMB_RS05470 0.72 -1.8 1,133,792 - AMB_RS05470 0.72 -0.1 1,133,948 + -1.4 1,133,948 + +0.1 1,133,949 - -0.3 1,133,957 + -2.6 1,133,957 + +0.6 1,133,958 - -0.1 1,133,958 - -0.6 1,134,007 + +1.9 1,134,007 + +0.4 1,134,034 + -1.6 1,134,036 + -0.1 1,134,036 + -0.6 1,134,037 - -0.5 1,134,051 + -0.3 1,134,173 - AMB_RS05475 0.21 +0.1
Or see this region's nucleotide sequence