Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04300 and AMB_RS04305 are separated by 181 nucleotides AMB_RS04305 and AMB_RS04310 are separated by 12 nucleotides
AMB_RS04300: AMB_RS04300 - beta-ketoacyl-ACP reductase, at 903,086 to 903,808
_RS04300
AMB_RS04305: AMB_RS04305 - HD domain-containing protein, at 903,990 to 905,198
_RS04305
AMB_RS04310: AMB_RS04310 - receptor protein, at 905,211 to 907,154
_RS04310
Position (kb)
903
904
905
906 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 903.803 kb on + strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.804 kb on - strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.938 kb on + strand at 903.939 kb on - strand at 903.939 kb on - strand at 903.999 kb on - strand at 903.999 kb on - strand at 904.001 kb on + strand at 904.002 kb on - strand at 904.002 kb on - strand at 904.002 kb on - strand at 904.002 kb on - strand at 904.040 kb on + strand at 904.040 kb on + strand at 904.040 kb on + strand at 904.040 kb on + strand at 904.041 kb on - strand at 904.041 kb on - strand at 904.041 kb on - strand at 904.103 kb on + strand at 904.104 kb on - strand at 904.194 kb on + strand, within AMB_RS04305 at 904.343 kb on + strand, within AMB_RS04305 at 904.343 kb on + strand, within AMB_RS04305 at 904.344 kb on - strand, within AMB_RS04305 at 904.392 kb on + strand, within AMB_RS04305 at 904.392 kb on + strand, within AMB_RS04305 at 904.392 kb on + strand, within AMB_RS04305 at 904.392 kb on + strand, within AMB_RS04305 at 904.393 kb on - strand, within AMB_RS04305 at 904.393 kb on - strand, within AMB_RS04305 at 904.393 kb on - strand, within AMB_RS04305 at 904.393 kb on - strand, within AMB_RS04305 at 904.584 kb on + strand, within AMB_RS04305 at 904.584 kb on + strand, within AMB_RS04305 at 904.680 kb on + strand, within AMB_RS04305 at 904.680 kb on + strand, within AMB_RS04305 at 904.680 kb on + strand, within AMB_RS04305 at 904.680 kb on + strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.681 kb on - strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.686 kb on + strand, within AMB_RS04305 at 904.687 kb on - strand, within AMB_RS04305 at 904.687 kb on - strand, within AMB_RS04305 at 904.872 kb on - strand, within AMB_RS04305 at 904.872 kb on - strand, within AMB_RS04305 at 904.896 kb on + strand, within AMB_RS04305 at 904.896 kb on + strand, within AMB_RS04305 at 904.978 kb on - strand, within AMB_RS04305 at 905.040 kb on - strand, within AMB_RS04305 at 905.070 kb on + strand, within AMB_RS04305 at 905.071 kb on - strand, within AMB_RS04305 at 905.308 kb on + strand at 905.308 kb on + strand at 905.308 kb on + strand at 905.309 kb on - strand at 905.443 kb on + strand, within AMB_RS04310 at 905.443 kb on + strand, within AMB_RS04310 at 905.443 kb on + strand, within AMB_RS04310 at 905.443 kb on + strand, within AMB_RS04310 at 905.444 kb on - strand, within AMB_RS04310 at 905.444 kb on - strand, within AMB_RS04310 at 905.677 kb on + strand, within AMB_RS04310 at 905.678 kb on - strand, within AMB_RS04310 at 905.696 kb on - strand, within AMB_RS04310 at 905.696 kb on - strand, within AMB_RS04310 at 905.779 kb on + strand, within AMB_RS04310 at 905.780 kb on - strand, within AMB_RS04310 at 905.944 kb on + strand, within AMB_RS04310 at 905.944 kb on + strand, within AMB_RS04310 at 905.944 kb on + strand, within AMB_RS04310 at 906.091 kb on + strand, within AMB_RS04310 at 906.092 kb on - strand, within AMB_RS04310 at 906.092 kb on - strand, within AMB_RS04310
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 903,803 + -0.9 903,804 - -0.9 903,804 - -0.3 903,804 - -0.7 903,804 - -1.4 903,804 - -0.3 903,938 + +0.4 903,938 + -1.4 903,938 + +0.7 903,938 + -1.6 903,939 - -1.0 903,939 - -0.3 903,999 - -0.0 903,999 - +0.1 904,001 + -0.1 904,002 - -0.2 904,002 - -1.6 904,002 - +1.1 904,002 - -0.1 904,040 + +0.2 904,040 + +3.5 904,040 + -2.1 904,040 + -0.2 904,041 - -0.3 904,041 - +0.6 904,041 - -0.5 904,103 + -0.9 904,104 - -0.7 904,194 + AMB_RS04305 0.17 -0.9 904,343 + AMB_RS04305 0.29 -0.1 904,343 + AMB_RS04305 0.29 +0.1 904,344 - AMB_RS04305 0.29 +0.2 904,392 + AMB_RS04305 0.33 -0.1 904,392 + AMB_RS04305 0.33 +0.7 904,392 + AMB_RS04305 0.33 +0.7 904,392 + AMB_RS04305 0.33 -0.9 904,393 - AMB_RS04305 0.33 -0.3 904,393 - AMB_RS04305 0.33 +0.4 904,393 - AMB_RS04305 0.33 -0.5 904,393 - AMB_RS04305 0.33 -1.7 904,584 + AMB_RS04305 0.49 -1.4 904,584 + AMB_RS04305 0.49 -0.3 904,680 + AMB_RS04305 0.57 -0.6 904,680 + AMB_RS04305 0.57 -0.8 904,680 + AMB_RS04305 0.57 -0.2 904,680 + AMB_RS04305 0.57 -0.3 904,681 - AMB_RS04305 0.57 -0.3 904,681 - AMB_RS04305 0.57 -0.4 904,681 - AMB_RS04305 0.57 +0.3 904,681 - AMB_RS04305 0.57 -1.0 904,681 - AMB_RS04305 0.57 -0.1 904,681 - AMB_RS04305 0.57 -0.4 904,686 + AMB_RS04305 0.58 +1.0 904,686 + AMB_RS04305 0.58 -0.5 904,686 + AMB_RS04305 0.58 +0.2 904,686 + AMB_RS04305 0.58 -0.7 904,686 + AMB_RS04305 0.58 -1.0 904,686 + AMB_RS04305 0.58 +0.0 904,686 + AMB_RS04305 0.58 +0.2 904,686 + AMB_RS04305 0.58 -0.5 904,687 - AMB_RS04305 0.58 -1.8 904,687 - AMB_RS04305 0.58 +0.2 904,872 - AMB_RS04305 0.73 +0.5 904,872 - AMB_RS04305 0.73 +0.4 904,896 + AMB_RS04305 0.75 +0.2 904,896 + AMB_RS04305 0.75 +0.2 904,978 - AMB_RS04305 0.82 +0.5 905,040 - AMB_RS04305 0.87 -0.3 905,070 + AMB_RS04305 0.89 -0.3 905,071 - AMB_RS04305 0.89 +0.1 905,308 + +0.1 905,308 + -0.3 905,308 + -0.8 905,309 - +0.5 905,443 + AMB_RS04310 0.12 +0.3 905,443 + AMB_RS04310 0.12 +0.0 905,443 + AMB_RS04310 0.12 -0.2 905,443 + AMB_RS04310 0.12 +0.1 905,444 - AMB_RS04310 0.12 -1.2 905,444 - AMB_RS04310 0.12 -0.1 905,677 + AMB_RS04310 0.24 +0.4 905,678 - AMB_RS04310 0.24 +0.0 905,696 - AMB_RS04310 0.25 -0.6 905,696 - AMB_RS04310 0.25 +1.0 905,779 + AMB_RS04310 0.29 +0.1 905,780 - AMB_RS04310 0.29 -1.7 905,944 + AMB_RS04310 0.38 +0.1 905,944 + AMB_RS04310 0.38 +1.0 905,944 + AMB_RS04310 0.38 +0.0 906,091 + AMB_RS04310 0.45 -0.0 906,092 - AMB_RS04310 0.45 -2.0 906,092 - AMB_RS04310 0.45 -1.6
Or see this region's nucleotide sequence