Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04275 and AMB_RS04280 are separated by 37 nucleotides AMB_RS04280 and AMB_RS04285 are separated by 130 nucleotides AMB_RS04285 and AMB_RS04290 overlap by 71 nucleotides
AMB_RS04275: AMB_RS04275 - insulinase family protein, at 898,003 to 899,415
_RS04275
AMB_RS04280: AMB_RS04280 - polyhydroxyalkanoate synthesis repressor PhaR, at 899,453 to 900,016
_RS04280
AMB_RS04285: AMB_RS04285 - hypothetical protein, at 900,147 to 900,596
_RS04285
AMB_RS04290: AMB_RS04290 - poly-beta-hydroxybutyrate polymerase, at 900,526 to 901,632
_RS04290
Position (kb) 900
901 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 899.200 kb on + strand, within AMB_RS04275 at 899.201 kb on - strand, within AMB_RS04275 at 899.454 kb on + strand at 899.455 kb on - strand at 899.455 kb on - strand at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.748 kb on + strand, within AMB_RS04280 at 899.848 kb on + strand, within AMB_RS04280 at 899.929 kb on - strand, within AMB_RS04280 at 899.968 kb on - strand at 900.073 kb on + strand at 900.073 kb on + strand at 900.074 kb on - strand at 900.074 kb on - strand at 900.076 kb on + strand at 900.087 kb on + strand at 900.087 kb on + strand at 900.087 kb on + strand at 900.146 kb on + strand at 900.171 kb on + strand at 900.171 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 899,200 + AMB_RS04275 0.85 -0.9 899,201 - AMB_RS04275 0.85 -0.2 899,454 + -0.7 899,455 - +0.1 899,455 - +0.1 899,553 + AMB_RS04280 0.18 -1.6 899,553 + AMB_RS04280 0.18 -1.1 899,553 + AMB_RS04280 0.18 -1.2 899,553 + AMB_RS04280 0.18 -1.2 899,553 + AMB_RS04280 0.18 -1.2 899,553 + AMB_RS04280 0.18 -2.3 899,553 + AMB_RS04280 0.18 -1.1 899,553 + AMB_RS04280 0.18 -0.9 899,553 + AMB_RS04280 0.18 -1.0 899,553 + AMB_RS04280 0.18 -2.0 899,554 - AMB_RS04280 0.18 -1.1 899,554 - AMB_RS04280 0.18 -1.1 899,554 - AMB_RS04280 0.18 -1.2 899,554 - AMB_RS04280 0.18 -1.1 899,554 - AMB_RS04280 0.18 -2.8 899,554 - AMB_RS04280 0.18 -1.2 899,554 - AMB_RS04280 0.18 -0.9 899,554 - AMB_RS04280 0.18 -0.6 899,554 - AMB_RS04280 0.18 -1.1 899,554 - AMB_RS04280 0.18 -1.5 899,554 - AMB_RS04280 0.18 -0.3 899,554 - AMB_RS04280 0.18 -1.5 899,554 - AMB_RS04280 0.18 -1.8 899,554 - AMB_RS04280 0.18 -2.3 899,554 - AMB_RS04280 0.18 -0.9 899,554 - AMB_RS04280 0.18 -1.4 899,554 - AMB_RS04280 0.18 -0.9 899,659 + AMB_RS04280 0.37 -1.6 899,659 + AMB_RS04280 0.37 -2.0 899,659 + AMB_RS04280 0.37 -1.0 899,659 + AMB_RS04280 0.37 -1.0 899,659 + AMB_RS04280 0.37 -1.3 899,659 + AMB_RS04280 0.37 -1.3 899,659 + AMB_RS04280 0.37 -0.6 899,659 + AMB_RS04280 0.37 -0.8 899,659 + AMB_RS04280 0.37 -4.0 899,659 + AMB_RS04280 0.37 -1.4 899,659 + AMB_RS04280 0.37 +0.4 899,659 + AMB_RS04280 0.37 -2.2 899,659 + AMB_RS04280 0.37 -0.7 899,660 - AMB_RS04280 0.37 -4.1 899,660 - AMB_RS04280 0.37 -2.8 899,660 - AMB_RS04280 0.37 -0.5 899,660 - AMB_RS04280 0.37 -5.3 899,660 - AMB_RS04280 0.37 -1.6 899,660 - AMB_RS04280 0.37 -3.1 899,660 - AMB_RS04280 0.37 -1.6 899,661 + AMB_RS04280 0.37 +1.4 899,661 + AMB_RS04280 0.37 -1.6 899,661 + AMB_RS04280 0.37 -3.7 899,662 - AMB_RS04280 0.37 -1.1 899,662 - AMB_RS04280 0.37 +0.4 899,662 - AMB_RS04280 0.37 -1.4 899,662 - AMB_RS04280 0.37 -0.6 899,748 + AMB_RS04280 0.52 +0.4 899,848 + AMB_RS04280 0.70 -1.6 899,929 - AMB_RS04280 0.84 +0.4 899,968 - -2.2 900,073 + -2.0 900,073 + -1.2 900,074 - +0.4 900,074 - -1.4 900,076 + -2.2 900,087 + -2.9 900,087 + -1.6 900,087 + -3.0 900,146 + +1.0 900,171 + -0.2 900,171 + -0.8
Or see this region's nucleotide sequence