Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04130 and AMB_RS04135 overlap by 4 nucleotides AMB_RS04135 and AMB_RS04140 are separated by 34 nucleotides AMB_RS04140 and AMB_RS04145 are separated by 109 nucleotides AMB_RS04145 and AMB_RS04150 are separated by 7 nucleotides
AMB_RS04130: AMB_RS04130 - TRAP transporter large permease, at 865,520 to 866,848
_RS04130
AMB_RS04135: AMB_RS04135 - TRAP transporter small permease, at 866,845 to 867,378
_RS04135
AMB_RS04140: AMB_RS04140 - TRAP-type C4-dicarboxylate transport system, at 867,413 to 868,528
_RS04140
AMB_RS04145: AMB_RS04145 - homoprotocatechuate degradation operon regulator HpaR, at 868,638 to 869,129
_RS04145
AMB_RS04150: AMB_RS04150 - acetylornithine transaminase, at 869,137 to 870,303
_RS04150
Position (kb) 867
868
869 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 866.433 kb on + strand, within AMB_RS04130 at 866.433 kb on + strand, within AMB_RS04130 at 866.433 kb on + strand, within AMB_RS04130 at 866.434 kb on - strand, within AMB_RS04130 at 866.541 kb on + strand, within AMB_RS04130 at 866.541 kb on + strand, within AMB_RS04130 at 866.542 kb on - strand, within AMB_RS04130 at 866.542 kb on - strand, within AMB_RS04130 at 866.664 kb on + strand, within AMB_RS04130 at 866.665 kb on - strand, within AMB_RS04130 at 866.682 kb on + strand, within AMB_RS04130 at 866.742 kb on + strand at 866.742 kb on + strand at 866.742 kb on + strand at 866.742 kb on + strand at 866.742 kb on + strand at 866.743 kb on - strand at 866.743 kb on - strand at 866.743 kb on - strand at 866.892 kb on - strand at 866.892 kb on - strand at 867.391 kb on + strand at 867.392 kb on - strand at 867.432 kb on + strand at 867.433 kb on - strand at 867.433 kb on - strand at 867.601 kb on - strand, within AMB_RS04140 at 867.711 kb on + strand, within AMB_RS04140 at 867.712 kb on - strand, within AMB_RS04140 at 867.786 kb on + strand, within AMB_RS04140 at 867.786 kb on + strand, within AMB_RS04140 at 867.787 kb on - strand, within AMB_RS04140 at 867.787 kb on - strand, within AMB_RS04140 at 867.994 kb on + strand, within AMB_RS04140 at 867.995 kb on - strand, within AMB_RS04140 at 868.047 kb on + strand, within AMB_RS04140 at 868.048 kb on - strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.080 kb on + strand, within AMB_RS04140 at 868.081 kb on - strand, within AMB_RS04140 at 868.084 kb on - strand, within AMB_RS04140 at 868.173 kb on + strand, within AMB_RS04140 at 868.459 kb on + strand at 868.459 kb on + strand at 868.460 kb on - strand at 868.460 kb on - strand at 868.531 kb on + strand at 868.531 kb on + strand at 868.532 kb on - strand at 868.532 kb on - strand at 869.010 kb on + strand, within AMB_RS04145 at 869.056 kb on + strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.057 kb on - strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.058 kb on + strand, within AMB_RS04145 at 869.059 kb on - strand, within AMB_RS04145 at 869.059 kb on - strand, within AMB_RS04145 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.418 kb on + strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.419 kb on - strand, within AMB_RS04150 at 869.421 kb on + strand, within AMB_RS04150
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 866,433 + AMB_RS04130 0.69 -0.3 866,433 + AMB_RS04130 0.69 -1.8 866,433 + AMB_RS04130 0.69 +0.0 866,434 - AMB_RS04130 0.69 -0.0 866,541 + AMB_RS04130 0.77 -0.6 866,541 + AMB_RS04130 0.77 +0.3 866,542 - AMB_RS04130 0.77 +0.4 866,542 - AMB_RS04130 0.77 +0.4 866,664 + AMB_RS04130 0.86 -0.2 866,665 - AMB_RS04130 0.86 +0.4 866,682 + AMB_RS04130 0.87 -0.4 866,742 + -0.1 866,742 + -0.1 866,742 + -0.4 866,742 + -0.2 866,742 + -0.3 866,743 - +1.0 866,743 - +0.1 866,743 - +1.2 866,892 - -0.4 866,892 - -2.2 867,391 + +0.4 867,392 - +1.1 867,432 + +0.2 867,433 - +0.9 867,433 - -0.7 867,601 - AMB_RS04140 0.17 -0.2 867,711 + AMB_RS04140 0.27 +0.3 867,712 - AMB_RS04140 0.27 +1.1 867,786 + AMB_RS04140 0.33 -0.1 867,786 + AMB_RS04140 0.33 +0.6 867,787 - AMB_RS04140 0.34 -0.3 867,787 - AMB_RS04140 0.34 -0.3 867,994 + AMB_RS04140 0.52 +0.1 867,995 - AMB_RS04140 0.52 -0.7 868,047 + AMB_RS04140 0.57 -0.5 868,048 - AMB_RS04140 0.57 -0.5 868,080 + AMB_RS04140 0.60 -0.2 868,080 + AMB_RS04140 0.60 -0.6 868,080 + AMB_RS04140 0.60 -0.8 868,080 + AMB_RS04140 0.60 -1.2 868,080 + AMB_RS04140 0.60 -2.0 868,080 + AMB_RS04140 0.60 -0.5 868,080 + AMB_RS04140 0.60 -0.5 868,081 - AMB_RS04140 0.60 +0.7 868,084 - AMB_RS04140 0.60 -0.9 868,173 + AMB_RS04140 0.68 -2.1 868,459 + -0.3 868,459 + -0.5 868,460 - +0.0 868,460 - -0.1 868,531 + -0.0 868,531 + +0.1 868,532 - -0.3 868,532 - -1.2 869,010 + AMB_RS04145 0.76 -3.1 869,056 + AMB_RS04145 0.85 -0.6 869,057 - AMB_RS04145 0.85 +0.4 869,057 - AMB_RS04145 0.85 +0.4 869,057 - AMB_RS04145 0.85 +0.4 869,058 + AMB_RS04145 0.85 -0.1 869,058 + AMB_RS04145 0.85 -1.2 869,058 + AMB_RS04145 0.85 -0.1 869,059 - AMB_RS04145 0.86 -0.4 869,059 - AMB_RS04145 0.86 -0.2 869,418 + AMB_RS04150 0.24 +2.4 869,418 + AMB_RS04150 0.24 -0.5 869,418 + AMB_RS04150 0.24 -1.2 869,418 + AMB_RS04150 0.24 -0.0 869,419 - AMB_RS04150 0.24 -0.1 869,419 - AMB_RS04150 0.24 -0.4 869,419 - AMB_RS04150 0.24 -0.2 869,421 + AMB_RS04150 0.24 -1.0
Or see this region's nucleotide sequence