Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03155 and AMB_RS03160 overlap by 4 nucleotides AMB_RS03160 and AMB_RS03165 are separated by 198 nucleotides AMB_RS03165 and AMB_RS03170 are separated by 39 nucleotides AMB_RS03170 and AMB_RS03175 are separated by 29 nucleotides AMB_RS03175 and AMB_RS03180 are separated by 15 nucleotides AMB_RS03180 and AMB_RS03185 are separated by 1 nucleotides
AMB_RS03155: AMB_RS03155 - hypothetical protein, at 658,740 to 659,006
_RS03155
AMB_RS03160: AMB_RS03160 - flagellar protein FhlB, at 659,003 to 659,356
_RS03160
AMB_RS03165: AMB_RS03165 - flagellar basal body rod protein FlgB, at 659,555 to 659,956
_RS03165
AMB_RS03170: AMB_RS03170 - flagellar basal body rod protein FlgC, at 659,996 to 660,406
_RS03170
AMB_RS03175: AMB_RS03175 - flagellar hook-basal body protein FliE, at 660,436 to 660,747
_RS03175
AMB_RS03180: AMB_RS03180 - flagellar biosynthetic protein FliQ, at 660,763 to 661,032
_RS03180
AMB_RS03185: AMB_RS03185 - flagellar type III secretion system protein FliR, at 661,034 to 661,798
_RS03185
Position (kb) 659
660
661 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 659.055 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.061 kb on + strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.062 kb on - strand, within AMB_RS03160 at 659.419 kb on + strand at 659.419 kb on + strand at 659.420 kb on - strand at 659.469 kb on - strand at 659.478 kb on + strand at 659.479 kb on - strand at 659.495 kb on + strand at 659.532 kb on + strand at 659.532 kb on + strand at 659.558 kb on + strand at 659.558 kb on + strand at 659.558 kb on + strand at 659.559 kb on - strand at 659.653 kb on + strand, within AMB_RS03165 at 659.653 kb on + strand, within AMB_RS03165 at 659.664 kb on - strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.758 kb on + strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.759 kb on - strand, within AMB_RS03165 at 659.859 kb on + strand, within AMB_RS03165 at 659.860 kb on - strand, within AMB_RS03165 at 659.860 kb on - strand, within AMB_RS03165 at 659.888 kb on + strand, within AMB_RS03165 at 659.888 kb on + strand, within AMB_RS03165 at 659.888 kb on + strand, within AMB_RS03165 at 659.889 kb on - strand, within AMB_RS03165 at 659.889 kb on - strand, within AMB_RS03165 at 659.919 kb on - strand at 659.955 kb on - strand at 660.016 kb on + strand at 660.269 kb on + strand, within AMB_RS03170 at 660.269 kb on + strand, within AMB_RS03170 at 660.269 kb on + strand, within AMB_RS03170 at 660.269 kb on + strand, within AMB_RS03170 at 660.270 kb on - strand, within AMB_RS03170 at 660.270 kb on - strand, within AMB_RS03170 at 660.270 kb on - strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.335 kb on + strand, within AMB_RS03170 at 660.336 kb on - strand, within AMB_RS03170 at 660.336 kb on - strand, within AMB_RS03170 at 660.469 kb on + strand, within AMB_RS03175 at 660.470 kb on - strand, within AMB_RS03175 at 660.470 kb on - strand, within AMB_RS03175 at 660.471 kb on + strand, within AMB_RS03175 at 660.471 kb on + strand, within AMB_RS03175 at 660.472 kb on - strand, within AMB_RS03175 at 660.472 kb on - strand, within AMB_RS03175 at 660.718 kb on + strand at 660.718 kb on + strand at 660.719 kb on - strand at 660.719 kb on - strand at 660.719 kb on - strand at 660.720 kb on + strand at 660.959 kb on + strand, within AMB_RS03180 at 660.960 kb on - strand, within AMB_RS03180 at 661.084 kb on + strand at 661.085 kb on - strand at 661.085 kb on - strand at 661.085 kb on - strand at 661.155 kb on + strand, within AMB_RS03185 at 661.277 kb on + strand, within AMB_RS03185 at 661.278 kb on - strand, within AMB_RS03185 at 661.278 kb on - strand, within AMB_RS03185 at 661.278 kb on - strand, within AMB_RS03185 at 661.340 kb on + strand, within AMB_RS03185
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 659,055 + AMB_RS03160 0.15 -0.6 659,061 + AMB_RS03160 0.16 +0.6 659,061 + AMB_RS03160 0.16 -0.9 659,061 + AMB_RS03160 0.16 +0.2 659,061 + AMB_RS03160 0.16 -0.0 659,061 + AMB_RS03160 0.16 -0.4 659,061 + AMB_RS03160 0.16 -0.1 659,061 + AMB_RS03160 0.16 -0.3 659,061 + AMB_RS03160 0.16 -0.4 659,061 + AMB_RS03160 0.16 -0.6 659,061 + AMB_RS03160 0.16 -0.1 659,062 - AMB_RS03160 0.17 -0.9 659,062 - AMB_RS03160 0.17 -0.2 659,062 - AMB_RS03160 0.17 +0.1 659,062 - AMB_RS03160 0.17 -0.5 659,062 - AMB_RS03160 0.17 -2.0 659,062 - AMB_RS03160 0.17 +0.6 659,062 - AMB_RS03160 0.17 +0.1 659,062 - AMB_RS03160 0.17 -1.1 659,419 + -0.3 659,419 + -0.1 659,420 - +0.9 659,469 - -1.1 659,478 + +1.2 659,479 - -0.1 659,495 + -0.5 659,532 + -0.1 659,532 + -1.6 659,558 + -0.5 659,558 + -0.4 659,558 + -0.6 659,559 - -1.0 659,653 + AMB_RS03165 0.24 -0.6 659,653 + AMB_RS03165 0.24 +0.0 659,664 - AMB_RS03165 0.27 +0.0 659,758 + AMB_RS03165 0.50 -0.0 659,758 + AMB_RS03165 0.50 -0.0 659,758 + AMB_RS03165 0.50 -0.5 659,758 + AMB_RS03165 0.50 -0.2 659,758 + AMB_RS03165 0.50 -0.2 659,758 + AMB_RS03165 0.50 -1.6 659,758 + AMB_RS03165 0.50 -0.1 659,758 + AMB_RS03165 0.50 -0.3 659,758 + AMB_RS03165 0.50 -0.6 659,759 - AMB_RS03165 0.51 -0.4 659,759 - AMB_RS03165 0.51 -0.2 659,759 - AMB_RS03165 0.51 -0.4 659,759 - AMB_RS03165 0.51 +0.1 659,759 - AMB_RS03165 0.51 -2.9 659,759 - AMB_RS03165 0.51 -1.5 659,759 - AMB_RS03165 0.51 +0.1 659,759 - AMB_RS03165 0.51 -0.1 659,759 - AMB_RS03165 0.51 -0.2 659,759 - AMB_RS03165 0.51 +0.7 659,759 - AMB_RS03165 0.51 -0.2 659,859 + AMB_RS03165 0.76 -0.5 659,860 - AMB_RS03165 0.76 -0.5 659,860 - AMB_RS03165 0.76 -0.1 659,888 + AMB_RS03165 0.83 +0.8 659,888 + AMB_RS03165 0.83 -0.5 659,888 + AMB_RS03165 0.83 -2.2 659,889 - AMB_RS03165 0.83 +0.2 659,889 - AMB_RS03165 0.83 +0.7 659,919 - -0.5 659,955 - -0.5 660,016 + +0.5 660,269 + AMB_RS03170 0.66 +1.4 660,269 + AMB_RS03170 0.66 +0.0 660,269 + AMB_RS03170 0.66 +0.6 660,269 + AMB_RS03170 0.66 -0.7 660,270 - AMB_RS03170 0.67 -0.7 660,270 - AMB_RS03170 0.67 -0.1 660,270 - AMB_RS03170 0.67 +0.9 660,335 + AMB_RS03170 0.82 -0.4 660,335 + AMB_RS03170 0.82 +0.2 660,335 + AMB_RS03170 0.82 -0.0 660,335 + AMB_RS03170 0.82 -0.6 660,335 + AMB_RS03170 0.82 +0.1 660,335 + AMB_RS03170 0.82 +0.5 660,335 + AMB_RS03170 0.82 -0.2 660,336 - AMB_RS03170 0.83 -0.8 660,336 - AMB_RS03170 0.83 -0.5 660,469 + AMB_RS03175 0.11 -0.4 660,470 - AMB_RS03175 0.11 -1.2 660,470 - AMB_RS03175 0.11 -1.2 660,471 + AMB_RS03175 0.11 -0.0 660,471 + AMB_RS03175 0.11 -0.5 660,472 - AMB_RS03175 0.12 -0.2 660,472 - AMB_RS03175 0.12 +0.3 660,718 + +0.2 660,718 + -0.5 660,719 - -0.3 660,719 - -0.5 660,719 - +0.4 660,720 + -1.0 660,959 + AMB_RS03180 0.73 +0.4 660,960 - AMB_RS03180 0.73 +0.2 661,084 + -0.1 661,085 - +0.7 661,085 - -0.5 661,085 - +0.4 661,155 + AMB_RS03185 0.16 +0.2 661,277 + AMB_RS03185 0.32 -0.9 661,278 - AMB_RS03185 0.32 +1.4 661,278 - AMB_RS03185 0.32 -1.4 661,278 - AMB_RS03185 0.32 -0.8 661,340 + AMB_RS03185 0.40 -0.9
Or see this region's nucleotide sequence