Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01740 and AMB_RS01745 are separated by 95 nucleotides AMB_RS01745 and AMB_RS01750 overlap by 4 nucleotides AMB_RS01750 and AMB_RS01755 overlap by 4 nucleotides AMB_RS01755 and AMB_RS01760 overlap by 4 nucleotides AMB_RS01760 and AMB_RS23100 are separated by 17 nucleotides
AMB_RS01740: AMB_RS01740 - hypothetical protein, at 381,589 to 381,834
_RS01740
AMB_RS01745: AMB_RS01745 - hypothetical protein, at 381,930 to 382,334
_RS01745
AMB_RS01750: AMB_RS01750 - recombinase family protein, at 382,331 to 383,662
_RS01750
AMB_RS01755: AMB_RS01755 - DUF2924 domain-containing protein, at 383,659 to 384,108
_RS01755
AMB_RS01760: AMB_RS01760 - hypothetical protein, at 384,105 to 384,362
_RS01760
AMB_RS23100: AMB_RS23100 - hypothetical protein, at 384,380 to 385,057
_RS23100
Position (kb) 382
383
384 Strain fitness (log2 ratio) -2
-1
0
1 at 381.484 kb on + strand at 381.484 kb on + strand at 381.507 kb on + strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.508 kb on - strand at 381.544 kb on + strand at 381.544 kb on + strand at 381.545 kb on - strand at 381.644 kb on + strand, within AMB_RS01740 at 381.644 kb on + strand, within AMB_RS01740 at 381.645 kb on - strand, within AMB_RS01740 at 381.645 kb on - strand, within AMB_RS01740 at 381.752 kb on + strand, within AMB_RS01740 at 381.752 kb on + strand, within AMB_RS01740 at 381.753 kb on - strand, within AMB_RS01740 at 381.753 kb on - strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.800 kb on + strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.801 kb on - strand, within AMB_RS01740 at 381.896 kb on + strand at 381.896 kb on + strand at 381.897 kb on - strand at 382.075 kb on + strand, within AMB_RS01745 at 382.076 kb on - strand, within AMB_RS01745 at 382.090 kb on + strand, within AMB_RS01745 at 382.090 kb on + strand, within AMB_RS01745 at 382.345 kb on + strand at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.690 kb on + strand, within AMB_RS01750 at 382.691 kb on - strand, within AMB_RS01750 at 382.691 kb on - strand, within AMB_RS01750 at 382.691 kb on - strand, within AMB_RS01750 at 382.691 kb on - strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.692 kb on + strand, within AMB_RS01750 at 382.693 kb on - strand, within AMB_RS01750 at 382.693 kb on - strand, within AMB_RS01750 at 382.693 kb on - strand, within AMB_RS01750 at 382.693 kb on - strand, within AMB_RS01750 at 382.693 kb on - strand, within AMB_RS01750 at 382.695 kb on + strand, within AMB_RS01750 at 382.696 kb on - strand, within AMB_RS01750 at 382.701 kb on + strand, within AMB_RS01750 at 382.929 kb on + strand, within AMB_RS01750 at 382.929 kb on + strand, within AMB_RS01750 at 382.929 kb on + strand, within AMB_RS01750 at 382.929 kb on + strand, within AMB_RS01750 at 382.930 kb on - strand, within AMB_RS01750 at 382.953 kb on + strand, within AMB_RS01750 at 383.020 kb on + strand, within AMB_RS01750 at 383.020 kb on + strand, within AMB_RS01750 at 383.020 kb on + strand, within AMB_RS01750 at 383.020 kb on + strand, within AMB_RS01750 at 383.021 kb on - strand, within AMB_RS01750 at 383.021 kb on - strand, within AMB_RS01750 at 383.151 kb on + strand, within AMB_RS01750 at 383.152 kb on - strand, within AMB_RS01750 at 383.152 kb on - strand, within AMB_RS01750 at 383.403 kb on + strand, within AMB_RS01750 at 383.404 kb on - strand, within AMB_RS01750 at 383.502 kb on + strand, within AMB_RS01750 at 383.502 kb on + strand, within AMB_RS01750 at 383.502 kb on + strand, within AMB_RS01750 at 383.502 kb on + strand, within AMB_RS01750 at 383.503 kb on - strand, within AMB_RS01750 at 383.550 kb on + strand at 383.550 kb on + strand at 383.550 kb on + strand at 383.551 kb on - strand at 383.551 kb on - strand at 383.551 kb on - strand at 383.628 kb on + strand at 383.628 kb on + strand at 383.628 kb on + strand at 383.628 kb on + strand at 383.628 kb on + strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 383.629 kb on - strand at 384.005 kb on + strand, within AMB_RS01755 at 384.005 kb on + strand, within AMB_RS01755 at 384.110 kb on + strand at 384.110 kb on + strand at 384.110 kb on + strand at 384.196 kb on - strand, within AMB_RS01760 at 384.196 kb on - strand, within AMB_RS01760 at 384.196 kb on - strand, within AMB_RS01760 at 384.333 kb on + strand, within AMB_RS01760 at 384.333 kb on + strand, within AMB_RS01760 at 384.334 kb on - strand, within AMB_RS01760 at 384.345 kb on + strand at 384.381 kb on + strand at 384.381 kb on + strand at 384.381 kb on + strand at 384.381 kb on + strand at 384.382 kb on - strand at 384.382 kb on - strand at 384.541 kb on + strand, within AMB_RS23100 at 384.541 kb on + strand, within AMB_RS23100 at 384.541 kb on + strand, within AMB_RS23100 at 384.542 kb on - strand, within AMB_RS23100 at 384.542 kb on - strand, within AMB_RS23100 at 384.579 kb on + strand, within AMB_RS23100 at 384.580 kb on - strand, within AMB_RS23100 at 384.580 kb on - strand, within AMB_RS23100 at 384.634 kb on + strand, within AMB_RS23100 at 384.635 kb on - strand, within AMB_RS23100 at 384.635 kb on - strand, within AMB_RS23100 at 384.655 kb on + strand, within AMB_RS23100 at 384.655 kb on + strand, within AMB_RS23100 at 384.656 kb on - strand, within AMB_RS23100 at 384.656 kb on - strand, within AMB_RS23100
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 381,484 + -0.3 381,484 + -0.4 381,507 + +0.3 381,508 - -0.4 381,508 - -1.9 381,508 - +0.6 381,508 - +0.0 381,508 - +1.0 381,508 - -0.2 381,508 - -0.3 381,544 + -1.0 381,544 + +0.1 381,545 - +0.1 381,644 + AMB_RS01740 0.22 -0.1 381,644 + AMB_RS01740 0.22 -1.1 381,645 - AMB_RS01740 0.23 -0.6 381,645 - AMB_RS01740 0.23 +0.3 381,752 + AMB_RS01740 0.66 -0.3 381,752 + AMB_RS01740 0.66 -0.2 381,753 - AMB_RS01740 0.67 -1.2 381,753 - AMB_RS01740 0.67 +0.4 381,800 + AMB_RS01740 0.86 -0.1 381,800 + AMB_RS01740 0.86 -0.6 381,800 + AMB_RS01740 0.86 -0.2 381,800 + AMB_RS01740 0.86 -0.2 381,800 + AMB_RS01740 0.86 -0.1 381,800 + AMB_RS01740 0.86 -0.2 381,800 + AMB_RS01740 0.86 -0.1 381,800 + AMB_RS01740 0.86 -0.0 381,800 + AMB_RS01740 0.86 +0.3 381,801 - AMB_RS01740 0.86 -0.1 381,801 - AMB_RS01740 0.86 +0.2 381,801 - AMB_RS01740 0.86 +1.0 381,801 - AMB_RS01740 0.86 -0.3 381,896 + +1.0 381,896 + -1.0 381,897 - -0.0 382,075 + AMB_RS01745 0.36 -1.4 382,076 - AMB_RS01745 0.36 +0.3 382,090 + AMB_RS01745 0.40 -0.7 382,090 + AMB_RS01745 0.40 +1.0 382,345 + -0.6 382,690 + AMB_RS01750 0.27 -1.1 382,690 + AMB_RS01750 0.27 -0.5 382,690 + AMB_RS01750 0.27 -0.1 382,690 + AMB_RS01750 0.27 -0.3 382,690 + AMB_RS01750 0.27 +0.1 382,690 + AMB_RS01750 0.27 -0.2 382,690 + AMB_RS01750 0.27 +0.2 382,690 + AMB_RS01750 0.27 -0.2 382,690 + AMB_RS01750 0.27 -0.1 382,691 - AMB_RS01750 0.27 -0.4 382,691 - AMB_RS01750 0.27 -0.5 382,691 - AMB_RS01750 0.27 +0.4 382,691 - AMB_RS01750 0.27 -0.4 382,692 + AMB_RS01750 0.27 -0.7 382,692 + AMB_RS01750 0.27 -0.3 382,692 + AMB_RS01750 0.27 +0.0 382,692 + AMB_RS01750 0.27 -0.1 382,692 + AMB_RS01750 0.27 -0.4 382,692 + AMB_RS01750 0.27 -2.2 382,693 - AMB_RS01750 0.27 +0.2 382,693 - AMB_RS01750 0.27 -0.8 382,693 - AMB_RS01750 0.27 -0.0 382,693 - AMB_RS01750 0.27 -0.3 382,693 - AMB_RS01750 0.27 +0.4 382,695 + AMB_RS01750 0.27 -0.2 382,696 - AMB_RS01750 0.27 -0.4 382,701 + AMB_RS01750 0.28 -0.3 382,929 + AMB_RS01750 0.45 -0.3 382,929 + AMB_RS01750 0.45 -0.1 382,929 + AMB_RS01750 0.45 -2.2 382,929 + AMB_RS01750 0.45 +0.7 382,930 - AMB_RS01750 0.45 +0.2 382,953 + AMB_RS01750 0.47 -0.3 383,020 + AMB_RS01750 0.52 -0.1 383,020 + AMB_RS01750 0.52 -1.6 383,020 + AMB_RS01750 0.52 -0.2 383,020 + AMB_RS01750 0.52 -0.6 383,021 - AMB_RS01750 0.52 +0.1 383,021 - AMB_RS01750 0.52 -0.4 383,151 + AMB_RS01750 0.62 +0.6 383,152 - AMB_RS01750 0.62 +0.1 383,152 - AMB_RS01750 0.62 -0.3 383,403 + AMB_RS01750 0.80 -0.2 383,404 - AMB_RS01750 0.81 -0.1 383,502 + AMB_RS01750 0.88 -0.5 383,502 + AMB_RS01750 0.88 -0.9 383,502 + AMB_RS01750 0.88 -0.5 383,502 + AMB_RS01750 0.88 +0.1 383,503 - AMB_RS01750 0.88 +0.2 383,550 + -0.9 383,550 + -0.4 383,550 + +0.6 383,551 - -0.0 383,551 - -0.2 383,551 - +1.4 383,628 + -0.2 383,628 + -0.7 383,628 + -1.2 383,628 + +0.4 383,628 + +0.4 383,629 - -0.2 383,629 - -0.0 383,629 - +0.3 383,629 - +0.3 383,629 - -1.1 383,629 - -1.4 383,629 - -0.2 383,629 - -0.3 383,629 - -1.3 383,629 - -0.1 383,629 - -0.2 384,005 + AMB_RS01755 0.77 -0.2 384,005 + AMB_RS01755 0.77 +0.1 384,110 + -0.9 384,110 + -1.1 384,110 + +0.4 384,196 - AMB_RS01760 0.35 -1.2 384,196 - AMB_RS01760 0.35 +0.6 384,196 - AMB_RS01760 0.35 -1.3 384,333 + AMB_RS01760 0.88 -0.1 384,333 + AMB_RS01760 0.88 -0.7 384,334 - AMB_RS01760 0.89 -1.0 384,345 + -0.7 384,381 + -0.3 384,381 + -0.0 384,381 + +0.2 384,381 + -0.6 384,382 - -0.6 384,382 - -0.6 384,541 + AMB_RS23100 0.24 +1.4 384,541 + AMB_RS23100 0.24 -0.5 384,541 + AMB_RS23100 0.24 -0.6 384,542 - AMB_RS23100 0.24 -0.0 384,542 - AMB_RS23100 0.24 +0.5 384,579 + AMB_RS23100 0.29 +0.8 384,580 - AMB_RS23100 0.29 +0.1 384,580 - AMB_RS23100 0.29 -0.2 384,634 + AMB_RS23100 0.37 -0.2 384,635 - AMB_RS23100 0.38 +0.5 384,635 - AMB_RS23100 0.38 -1.0 384,655 + AMB_RS23100 0.41 -0.3 384,655 + AMB_RS23100 0.41 +0.2 384,656 - AMB_RS23100 0.41 -0.2 384,656 - AMB_RS23100 0.41 +0.4
Or see this region's nucleotide sequence