Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01075 and AMB_RS01080 overlap by 470 nucleotides AMB_RS01080 and AMB_RS01085 are separated by 118 nucleotides
AMB_RS01075: AMB_RS01075 - phosphoribosylamine--glycine ligase, at 238,562 to 239,836
_RS01075
AMB_RS01080: AMB_RS01080 - exodeoxyribonuclease VII large subunit, at 239,367 to 241,433
_RS01080
AMB_RS01085: AMB_RS01085 - signal recognition particle-docking protein FtsY, at 241,552 to 242,550
_RS01085
Position (kb) 239
240
241
242 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.502 kb on + strand at 238.503 kb on - strand at 238.524 kb on + strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.525 kb on - strand at 238.563 kb on + strand at 238.563 kb on + strand at 239.870 kb on - strand, within AMB_RS01080 at 239.870 kb on - strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.078 kb on + strand, within AMB_RS01080 at 240.079 kb on - strand, within AMB_RS01080 at 241.219 kb on - strand, within AMB_RS01080 at 241.231 kb on + strand at 241.431 kb on + strand at 241.431 kb on + strand at 241.431 kb on + strand at 241.431 kb on + strand at 241.432 kb on - strand at 241.432 kb on - strand at 241.432 kb on - strand at 241.442 kb on + strand at 241.443 kb on - strand at 241.550 kb on - strand at 241.553 kb on + strand at 241.553 kb on + strand at 241.554 kb on - strand at 241.554 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 238,502 + -0.1 238,503 - -0.6 238,524 + -0.6 238,525 - -0.5 238,525 - +2.7 238,525 - -0.0 238,563 + -0.3 238,563 + -0.4 239,870 - AMB_RS01080 0.24 -0.7 239,870 - AMB_RS01080 0.24 -3.0 240,078 + AMB_RS01080 0.34 -0.3 240,078 + AMB_RS01080 0.34 +0.3 240,078 + AMB_RS01080 0.34 -0.6 240,079 - AMB_RS01080 0.34 +1.8 241,219 - AMB_RS01080 0.90 +0.2 241,231 + -1.1 241,431 + -0.2 241,431 + -0.4 241,431 + +0.1 241,431 + +0.2 241,432 - +0.1 241,432 - +0.5 241,432 - -0.8 241,442 + +0.1 241,443 - -0.4 241,550 - +2.0 241,553 + -0.8 241,553 + +0.1 241,554 - +0.4 241,554 - -1.1
Or see this region's nucleotide sequence