Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS01055 and AMB_RS01060 overlap by 1 nucleotides AMB_RS01060 and AMB_RS01065 overlap by 1 nucleotides AMB_RS01065 and AMB_RS01070 are separated by 151 nucleotides
AMB_RS01055: AMB_RS01055 - hydrogenase 4 subunit F, at 233,042 to 234,532
_RS01055
AMB_RS01060: AMB_RS01060 - hydrogenase-4 component E, at 234,532 to 235,194
_RS01060
AMB_RS01065: AMB_RS01065 - formate hydrogenlyase, at 235,194 to 236,141
_RS01065
AMB_RS01070: AMB_RS01070 - hydrogenase 4 subunit B, at 236,293 to 238,302
_RS01070
Position (kb) 235
236
237 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 234.198 kb on + strand, within AMB_RS01055 at 234.199 kb on - strand, within AMB_RS01055 at 234.199 kb on - strand, within AMB_RS01055 at 234.253 kb on - strand, within AMB_RS01055 at 234.266 kb on + strand, within AMB_RS01055 at 234.309 kb on + strand, within AMB_RS01055 at 234.310 kb on - strand, within AMB_RS01055 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.013 kb on + strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.014 kb on - strand, within AMB_RS01060 at 235.118 kb on + strand, within AMB_RS01060 at 235.119 kb on - strand, within AMB_RS01060 at 235.175 kb on + strand at 235.175 kb on + strand at 235.176 kb on - strand at 235.176 kb on - strand at 235.213 kb on + strand at 235.213 kb on + strand at 235.213 kb on + strand at 235.933 kb on + strand, within AMB_RS01065 at 235.934 kb on - strand, within AMB_RS01065 at 235.934 kb on - strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 235.999 kb on + strand, within AMB_RS01065 at 236.000 kb on - strand, within AMB_RS01065 at 236.000 kb on - strand, within AMB_RS01065 at 236.012 kb on - strand, within AMB_RS01065 at 236.360 kb on + strand at 236.360 kb on + strand at 236.361 kb on - strand at 236.361 kb on - strand at 236.361 kb on - strand at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.585 kb on + strand, within AMB_RS01070 at 236.680 kb on - strand, within AMB_RS01070 at 236.726 kb on + strand, within AMB_RS01070 at 236.727 kb on - strand, within AMB_RS01070
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 234,198 + AMB_RS01055 0.78 -0.2 234,199 - AMB_RS01055 0.78 -1.6 234,199 - AMB_RS01055 0.78 -0.7 234,253 - AMB_RS01055 0.81 +0.3 234,266 + AMB_RS01055 0.82 +0.4 234,309 + AMB_RS01055 0.85 -0.3 234,310 - AMB_RS01055 0.85 -0.7 235,013 + AMB_RS01060 0.73 -0.1 235,013 + AMB_RS01060 0.73 -1.0 235,013 + AMB_RS01060 0.73 -0.1 235,013 + AMB_RS01060 0.73 -0.7 235,013 + AMB_RS01060 0.73 +0.4 235,013 + AMB_RS01060 0.73 -0.3 235,013 + AMB_RS01060 0.73 +0.0 235,013 + AMB_RS01060 0.73 -1.0 235,013 + AMB_RS01060 0.73 +0.6 235,013 + AMB_RS01060 0.73 +1.1 235,014 - AMB_RS01060 0.73 -0.7 235,014 - AMB_RS01060 0.73 -0.2 235,014 - AMB_RS01060 0.73 -0.2 235,014 - AMB_RS01060 0.73 -0.4 235,014 - AMB_RS01060 0.73 -0.2 235,014 - AMB_RS01060 0.73 -0.6 235,118 + AMB_RS01060 0.88 -0.2 235,119 - AMB_RS01060 0.89 -0.0 235,175 + +0.1 235,175 + -0.4 235,176 - -0.4 235,176 - +0.1 235,213 + -0.9 235,213 + +0.3 235,213 + +1.3 235,933 + AMB_RS01065 0.78 +0.4 235,934 - AMB_RS01065 0.78 +0.8 235,934 - AMB_RS01065 0.78 -0.3 235,999 + AMB_RS01065 0.85 +0.4 235,999 + AMB_RS01065 0.85 +0.3 235,999 + AMB_RS01065 0.85 +0.1 235,999 + AMB_RS01065 0.85 -0.3 235,999 + AMB_RS01065 0.85 +0.5 236,000 - AMB_RS01065 0.85 -1.2 236,000 - AMB_RS01065 0.85 +0.1 236,012 - AMB_RS01065 0.86 -0.1 236,360 + -0.3 236,360 + -0.5 236,361 - -0.2 236,361 - -0.3 236,361 - -0.3 236,585 + AMB_RS01070 0.15 -0.1 236,585 + AMB_RS01070 0.15 -0.9 236,585 + AMB_RS01070 0.15 -1.3 236,585 + AMB_RS01070 0.15 -3.7 236,680 - AMB_RS01070 0.19 +0.4 236,726 + AMB_RS01070 0.22 -0.9 236,727 - AMB_RS01070 0.22 -1.4
Or see this region's nucleotide sequence