Experiment: 150_initial_1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS00720 and AMB_RS00725 overlap by 4 nucleotides AMB_RS00725 and AMB_RS00730 are separated by 3 nucleotides
AMB_RS00720: AMB_RS00720 - hypothetical protein, at 163,344 to 165,542
_RS00720
AMB_RS00725: AMB_RS00725 - glycosyltransferase family 9 protein, at 165,539 to 166,516
_RS00725
AMB_RS00730: AMB_RS00730 - nucleotide sugar dehydrogenase, at 166,520 to 167,791
_RS00730
Position (kb)
165
166
167 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 164.677 kb on + strand, within AMB_RS00720 at 164.677 kb on + strand, within AMB_RS00720 at 164.677 kb on + strand, within AMB_RS00720 at 164.677 kb on + strand, within AMB_RS00720 at 164.700 kb on + strand, within AMB_RS00720 at 164.700 kb on + strand, within AMB_RS00720 at 164.700 kb on + strand, within AMB_RS00720 at 164.700 kb on + strand, within AMB_RS00720 at 164.701 kb on - strand, within AMB_RS00720 at 164.701 kb on - strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.776 kb on + strand, within AMB_RS00720 at 164.777 kb on - strand, within AMB_RS00720 at 164.777 kb on - strand, within AMB_RS00720 at 164.777 kb on - strand, within AMB_RS00720 at 164.777 kb on - strand, within AMB_RS00720 at 164.777 kb on - strand, within AMB_RS00720 at 164.848 kb on + strand, within AMB_RS00720 at 164.848 kb on + strand, within AMB_RS00720 at 164.849 kb on - strand, within AMB_RS00720 at 164.849 kb on - strand, within AMB_RS00720 at 164.941 kb on + strand, within AMB_RS00720 at 164.941 kb on + strand, within AMB_RS00720 at 164.942 kb on - strand, within AMB_RS00720 at 164.995 kb on + strand, within AMB_RS00720 at 164.995 kb on + strand, within AMB_RS00720 at 164.995 kb on + strand, within AMB_RS00720 at 164.996 kb on - strand, within AMB_RS00720 at 164.996 kb on - strand, within AMB_RS00720 at 164.996 kb on - strand, within AMB_RS00720 at 165.035 kb on + strand, within AMB_RS00720 at 165.035 kb on + strand, within AMB_RS00720 at 165.036 kb on - strand, within AMB_RS00720 at 165.036 kb on - strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.037 kb on + strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.038 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.137 kb on - strand, within AMB_RS00720 at 165.295 kb on + strand, within AMB_RS00720 at 165.296 kb on - strand, within AMB_RS00720 at 165.325 kb on + strand at 165.325 kb on + strand at 165.325 kb on + strand at 165.325 kb on + strand at 165.325 kb on + strand at 165.326 kb on - strand at 165.326 kb on - strand at 165.326 kb on - strand at 165.329 kb on - strand at 165.373 kb on + strand at 165.373 kb on + strand at 165.374 kb on - strand at 165.397 kb on + strand at 165.397 kb on + strand at 165.398 kb on - strand at 165.398 kb on - strand at 165.398 kb on - strand at 165.398 kb on - strand at 165.488 kb on - strand at 165.488 kb on - strand at 165.543 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 150_initial_1 remove 164,677 + AMB_RS00720 0.61 -0.4 164,677 + AMB_RS00720 0.61 -0.7 164,677 + AMB_RS00720 0.61 -0.4 164,677 + AMB_RS00720 0.61 -0.2 164,700 + AMB_RS00720 0.62 -3.1 164,700 + AMB_RS00720 0.62 -0.3 164,700 + AMB_RS00720 0.62 -0.5 164,700 + AMB_RS00720 0.62 +0.2 164,701 - AMB_RS00720 0.62 +0.9 164,701 - AMB_RS00720 0.62 -0.2 164,776 + AMB_RS00720 0.65 -0.5 164,776 + AMB_RS00720 0.65 -1.2 164,776 + AMB_RS00720 0.65 -0.6 164,776 + AMB_RS00720 0.65 -0.4 164,776 + AMB_RS00720 0.65 -0.1 164,776 + AMB_RS00720 0.65 +1.3 164,776 + AMB_RS00720 0.65 -0.5 164,776 + AMB_RS00720 0.65 +0.6 164,777 - AMB_RS00720 0.65 +0.4 164,777 - AMB_RS00720 0.65 -1.0 164,777 - AMB_RS00720 0.65 -0.3 164,777 - AMB_RS00720 0.65 -0.1 164,777 - AMB_RS00720 0.65 +0.5 164,848 + AMB_RS00720 0.68 -0.7 164,848 + AMB_RS00720 0.68 -0.3 164,849 - AMB_RS00720 0.68 -0.2 164,849 - AMB_RS00720 0.68 +0.4 164,941 + AMB_RS00720 0.73 -0.3 164,941 + AMB_RS00720 0.73 -0.2 164,942 - AMB_RS00720 0.73 +1.2 164,995 + AMB_RS00720 0.75 -0.0 164,995 + AMB_RS00720 0.75 -0.0 164,995 + AMB_RS00720 0.75 +0.4 164,996 - AMB_RS00720 0.75 -0.0 164,996 - AMB_RS00720 0.75 +0.3 164,996 - AMB_RS00720 0.75 +0.1 165,035 + AMB_RS00720 0.77 -0.4 165,035 + AMB_RS00720 0.77 +0.4 165,036 - AMB_RS00720 0.77 -0.1 165,036 - AMB_RS00720 0.77 -0.2 165,037 + AMB_RS00720 0.77 -0.1 165,037 + AMB_RS00720 0.77 +0.9 165,037 + AMB_RS00720 0.77 -0.9 165,037 + AMB_RS00720 0.77 -0.2 165,037 + AMB_RS00720 0.77 -0.9 165,037 + AMB_RS00720 0.77 -0.5 165,037 + AMB_RS00720 0.77 +0.1 165,037 + AMB_RS00720 0.77 +0.1 165,037 + AMB_RS00720 0.77 -0.2 165,038 - AMB_RS00720 0.77 +0.4 165,038 - AMB_RS00720 0.77 +0.6 165,038 - AMB_RS00720 0.77 +0.1 165,038 - AMB_RS00720 0.77 -0.1 165,038 - AMB_RS00720 0.77 +0.8 165,038 - AMB_RS00720 0.77 -2.2 165,137 - AMB_RS00720 0.82 -0.1 165,137 - AMB_RS00720 0.82 +0.3 165,137 - AMB_RS00720 0.82 -0.6 165,137 - AMB_RS00720 0.82 -0.6 165,137 - AMB_RS00720 0.82 +0.0 165,137 - AMB_RS00720 0.82 -0.0 165,295 + AMB_RS00720 0.89 -0.6 165,296 - AMB_RS00720 0.89 +0.6 165,325 + -0.6 165,325 + +0.2 165,325 + -0.4 165,325 + -0.2 165,325 + -0.6 165,326 - +0.8 165,326 - -0.1 165,326 - -0.6 165,329 - +0.4 165,373 + -1.5 165,373 + -1.1 165,374 - -1.1 165,397 + +0.1 165,397 + -0.6 165,398 - -0.6 165,398 - -0.1 165,398 - +0.4 165,398 - +0.2 165,488 - -1.0 165,488 - -0.6 165,543 - -0.8
Or see this region's nucleotide sequence