Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS12590 and ABID97_RS12595 are separated by 83 nucleotides ABID97_RS12595 and ABID97_RS12600 are separated by 94 nucleotides ABID97_RS12600 and ABID97_RS12605 are separated by 299 nucleotides
ABID97_RS12590: ABID97_RS12590 - triphosphoribosyl-dephospho-CoA synthase, at 2,614,110 to 2,615,048
_RS12590
ABID97_RS12595: ABID97_RS12595 - formaldehyde-activating enzyme, at 2,615,132 to 2,615,644
_RS12595
ABID97_RS12600: ABID97_RS12600 - dihydroneopterin aldolase, at 2,615,739 to 2,616,170
_RS12600
ABID97_RS12605: ABID97_RS12605 - sigma-54-dependent Fis family transcriptional regulator, at 2,616,470 to 2,618,515
_RS12605
Position (kb) 2615
2616
2617 Strain fitness (log2 ratio) -1
0
1 at 2614.758 kb on + strand, within ABID97_RS12590 at 2614.758 kb on + strand, within ABID97_RS12590 at 2615.049 kb on + strand at 2615.088 kb on - strand at 2615.088 kb on - strand at 2615.197 kb on + strand, within ABID97_RS12595 at 2616.447 kb on - strand at 2616.713 kb on + strand, within ABID97_RS12605
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,614,758 + ABID97_RS12590 0.69 +0.7 2,614,758 + ABID97_RS12590 0.69 -0.4 2,615,049 + -0.1 2,615,088 - -1.5 2,615,088 - +0.1 2,615,197 + ABID97_RS12595 0.13 +1.4 2,616,447 - -0.6 2,616,713 + ABID97_RS12605 0.12 -0.4
Or see this region's nucleotide sequence