Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS12460 and ABID97_RS12465 are separated by 127 nucleotides ABID97_RS12465 and ABID97_RS12470 are separated by 82 nucleotides
ABID97_RS12460: ABID97_RS12460 - acetoacetyl-CoA reductase, at 2,581,200 to 2,581,937
_RS12460
ABID97_RS12465: ABID97_RS12465 - dicarboxylate/amino acid:cation symporter, at 2,582,065 to 2,583,408
_RS12465
ABID97_RS12470: ABID97_RS12470 - YbaL family putative K(+) efflux transporter, at 2,583,491 to 2,585,194
_RS12470
Position (kb)
2582
2583
2584 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2581.199 kb on + strand at 2581.218 kb on + strand at 2581.218 kb on + strand at 2581.218 kb on + strand at 2581.219 kb on - strand at 2581.219 kb on - strand at 2581.219 kb on - strand at 2581.219 kb on - strand at 2581.653 kb on + strand, within ABID97_RS12460 at 2581.654 kb on - strand, within ABID97_RS12460 at 2581.654 kb on - strand, within ABID97_RS12460 at 2581.750 kb on - strand, within ABID97_RS12460 at 2581.888 kb on - strand at 2582.034 kb on + strand at 2582.035 kb on - strand at 2582.119 kb on + strand at 2582.330 kb on - strand, within ABID97_RS12465 at 2582.330 kb on - strand, within ABID97_RS12465 at 2582.330 kb on - strand, within ABID97_RS12465 at 2582.450 kb on - strand, within ABID97_RS12465 at 2582.663 kb on - strand, within ABID97_RS12465 at 2582.770 kb on + strand, within ABID97_RS12465 at 2582.771 kb on - strand, within ABID97_RS12465 at 2582.771 kb on - strand, within ABID97_RS12465 at 2582.983 kb on + strand, within ABID97_RS12465
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 2,581,199 + +1.8 2,581,218 + +3.0 2,581,218 + +3.0 2,581,218 + +2.4 2,581,219 - +3.0 2,581,219 - +2.5 2,581,219 - +3.0 2,581,219 - +1.9 2,581,653 + ABID97_RS12460 0.61 +2.5 2,581,654 - ABID97_RS12460 0.62 +2.6 2,581,654 - ABID97_RS12460 0.62 +3.3 2,581,750 - ABID97_RS12460 0.75 +3.0 2,581,888 - +3.0 2,582,034 + +2.0 2,582,035 - +0.4 2,582,119 + -1.8 2,582,330 - ABID97_RS12465 0.20 -1.9 2,582,330 - ABID97_RS12465 0.20 -0.1 2,582,330 - ABID97_RS12465 0.20 -1.9 2,582,450 - ABID97_RS12465 0.29 -0.7 2,582,663 - ABID97_RS12465 0.44 -2.6 2,582,770 + ABID97_RS12465 0.52 -0.1 2,582,771 - ABID97_RS12465 0.53 -1.7 2,582,771 - ABID97_RS12465 0.53 -2.3 2,582,983 + ABID97_RS12465 0.68 -1.5
Or see this region's nucleotide sequence