Strain Fitness in Escherichia coli BW25113 around b0963

Experiment: D-Serine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthelD and mgsA are separated by 31 nucleotidesmgsA and yccT are separated by 95 nucleotidesyccT and b0965 are separated by 172 nucleotides b0962: helD - DNA helicase IV (NCBI), at 1,023,694 to 1,025,748 helD b0963: mgsA - methylglyoxal synthase (VIMSS), at 1,025,780 to 1,026,238 mgsA b0964: yccT - hypothetical protein (NCBI), at 1,026,334 to 1,026,996 yccT b0965: b0965 - orf, hypothetical protein (VIMSS), at 1,027,169 to 1,027,582 b0965 Position (kb) 1025 1026 1027Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1024.801 kb on - strand, within helDat 1024.801 kb on - strand, within helDat 1024.971 kb on + strand, within helDat 1024.988 kb on + strand, within helDat 1024.988 kb on + strand, within helDat 1025.201 kb on - strand, within helDat 1025.201 kb on - strand, within helDat 1025.213 kb on + strand, within helDat 1025.213 kb on + strand, within helDat 1025.399 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.424 kb on + strand, within helDat 1025.661 kb on + strandat 1025.662 kb on + strandat 1025.669 kb on - strandat 1025.772 kb on + strandat 1025.827 kb on - strand, within mgsAat 1025.893 kb on - strand, within mgsAat 1025.921 kb on + strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1025.943 kb on - strand, within mgsAat 1026.042 kb on - strand, within mgsAat 1026.043 kb on - strandat 1026.054 kb on + strand, within mgsAat 1026.115 kb on + strand, within mgsAat 1026.116 kb on - strand, within mgsAat 1026.119 kb on + strand, within mgsAat 1026.123 kb on - strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.153 kb on + strand, within mgsAat 1026.295 kb on + strandat 1026.403 kb on - strand, within yccTat 1026.403 kb on - strand, within yccTat 1026.627 kb on + strand, within yccTat 1026.627 kb on + strandat 1026.685 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.740 kb on + strand, within yccTat 1026.804 kb on + strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.824 kb on - strand, within yccTat 1026.958 kb on + strandat 1026.985 kb on + strandat 1026.985 kb on + strandat 1026.992 kb on + strandat 1027.060 kb on + strandat 1027.060 kb on + strandat 1027.085 kb on + strandat 1027.145 kb on + strandat 1027.153 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Serine (C)
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1,024,801 - helD b0962 0.54 -0.0
1,024,801 - helD b0962 0.54 -0.7
1,024,971 + helD b0962 0.62 -0.6
1,024,988 + helD b0962 0.63 +2.0
1,024,988 + helD b0962 0.63 -0.1
1,025,201 - helD b0962 0.73 -0.1
1,025,201 - helD b0962 0.73 +0.0
1,025,213 + helD b0962 0.74 -0.3
1,025,213 + helD b0962 0.74 -0.4
1,025,399 + helD b0962 0.83 -0.6
1,025,424 + helD b0962 0.84 -0.1
1,025,424 + helD b0962 0.84 -1.6
1,025,661 + -1.0
1,025,662 + -0.4
1,025,669 - -0.1
1,025,772 + -0.1
1,025,827 - mgsA b0963 0.10 +0.3
1,025,893 - mgsA b0963 0.25 +0.4
1,025,921 + mgsA b0963 0.31 -0.1
1,025,943 - mgsA b0963 0.36 -1.9
1,025,943 - mgsA b0963 0.36 +0.1
1,026,042 - mgsA b0963 0.57 -0.3
1,026,043 - +1.6
1,026,054 + mgsA b0963 0.60 -0.4
1,026,115 + mgsA b0963 0.73 -0.5
1,026,116 - mgsA b0963 0.73 -0.3
1,026,119 + mgsA b0963 0.74 -0.2
1,026,123 - mgsA b0963 0.75 +0.1
1,026,153 + mgsA b0963 0.81 +0.9
1,026,153 + mgsA b0963 0.81 +1.1
1,026,295 + +0.4
1,026,403 - yccT b0964 0.10 +0.0
1,026,403 - yccT b0964 0.10 +0.4
1,026,627 + yccT b0964 0.44 -1.1
1,026,627 + +1.4
1,026,685 + yccT b0964 0.53 -0.1
1,026,740 + yccT b0964 0.61 -0.4
1,026,740 + yccT b0964 0.61 -3.3
1,026,804 + yccT b0964 0.71 -0.1
1,026,824 - yccT b0964 0.74 -1.6
1,026,824 - yccT b0964 0.74 -1.1
1,026,958 + +0.1
1,026,985 + +0.1
1,026,985 + +0.2
1,026,992 + +0.6
1,027,060 + -0.2
1,027,060 + +0.6
1,027,085 + +0.0
1,027,145 + +1.5
1,027,153 - +0.4

Or see this region's nucleotide sequence